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Fluorescent dual α9β1/α4β1 integrin inhibitor. Comprises BOP (Cat. No. 6047) conjugated to Janelia Fluor® 549 (Cat. No. 6147). Fluorogenic: fluoresces only once bound to integrins, enabling hassle-free no-wash experiments. Bright and photostable, enabling live cell tracking of integrin receptors over long time-course experiments. Excitation maximum = 549 nm; emission maximum = 571 nm.
Application of BOP-JF549 in Immortalized Human Keratinocyte Cells
Immortalized human keratinocytes expressing a GFP-tagged α4 integrin were incubated with 10 μm BOP-Janelia Fluor® 568. Cells were incubated at 37°C and imaged using Nikon A1R confocal microscope. Scale bar = 10 μm.
Image kindly provided by Willow Hight-Warburton, Kings College London.
Optical Data for BOP-JF549
|Storage||Store at -20°C|
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