Janelia Fluor® 646, SE

Pricing Availability   Qty
Cat.No. 6148 - Janelia Fluor 646, SE | C33H31N3O6Si | CAS No. 1811539-59-9
Description: Cell permeable red fluorescent dye; supplied as NHS ester
Alternative Names: JF 646, NHS
Chemical Name: 1-[7-(1-Azetidinyl)-10-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt
Datasheet
Citations
Reviews (1)
Protocols (1)
Literature

Biological Activity

Fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Compatible with self-labeling tag systems (e.g. HaloTag® and SNAPtag® ). Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells), STED and PAINT imaging. Can be multiplexed for two colour imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54, Max. extinction coefficient = 152,000 M-1cm-1 (measured in Ethanol plus 0.1% TFA); A280 correction factor is 0.19.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Application of Janelia Fluor® Dyes in Cardiac Tissue

Application of Janelia Fluor® Dyes in Cardiac Tissue

Super-resolution image (dSTORM) of muscle tissue from the left ventricle of an adult pig heart. JF646 (Cat.No. 6148) was used to label collagen VI, which is present on the cell plasma membrane, and imaged with a 642 nm excitation laser. A clearer structure is achieved in comparison with the widefield image (top right). Scale bar 2 μm.

Image kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Alex Clowsley and Anna Meletiou.

Spectra charts
Emission color Red
Brightness
λabs 646 nm
λem 664 nm
Extinction coefficient (ε) 152,000 M-1cm-1
Quantum yield (φ) 0.54
Closest Laser line 640 nm
Alternative for Alexa Fluor™ 647, Atto 647, Atto 647N*, CF640R, CF647, Cyanine 5

Technical Data

M. Wt 593.71
Formula C33H31N3O6Si
Storage Store at -20°C
CAS Number 1811539-59-9
PubChem ID 124201858
InChI Key ODBCGJUBBYOJOS-UHFFFAOYSA-N
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3[Si]5(C)C)=C(C=C/6)C5=CC6=[N+]7CCC\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.87 20

Preparing Stock Solutions

The following data is based on the product molecular weight 593.71. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 8.42 mL 42.11 mL 84.22 mL
1 mM 1.68 mL 8.42 mL 16.84 mL
2 mM 0.84 mL 4.21 mL 8.42 mL
10 mM 0.17 mL 0.84 mL 1.68 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:

References

References are publications that support the biological activity of the product.

Schmidt et al (2016) Live cell imaging reveals the dynamics of telomerase recruitment to telomeres. Cell 166 1188 PMID: 27523609

Li et al (2016) Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. Elife 5 e17056 PMID: 27484239

Legant et al (2016) High-density three-dimensional localization microscopy across large volumes. Nat.Methods 13 359 PMID: 26950745

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551

Deng et al (2015) CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc.Natl.Acad.Sci.USA. 112 11870 PMID: 26324940

Ticau et al (2015) Single-molecule studies of origin licensing reveal mechanisms ensuring bidirectional helicase loading. Cell 161 513 PMID: 25892223

Hong et al (2009) Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome. Proc.Natl.Acad.Sci.U.S.A. 106 14276 PMID: 19666497


If you know of a relevant reference for Janelia Fluor® 646, SE, please let us know.

View Related Products by Target

Keywords: Janelia Fluor 646, SE, Janelia Fluor 646, SE supplier, JF646, Janelia, Fluor, 646-SE, fluorescent, dyes, NHS, ester, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, STED, JF, 646,, Dyes, 6148, Tocris Bioscience

Citations for Janelia Fluor® 646, SE

Citations are publications that use Tocris products.

Currently there are no citations for Janelia Fluor® 646, SE. Do you know of a great paper that uses Janelia Fluor® 646, SE from Tocris? Please let us know.

Reviews for Janelia Fluor® 646, SE

Average Rating: 4 (Based on 1 Review.)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Janelia Fluor® 646, SE?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


JF646 labeled proteins in vitro
By Saumya Saurabh on 02/26/2018
Assay Type: In Vitro
Species: Other

I used this dye for labeling bacterial proteins in vitro. The labeling method is pretty straight-forward as for any NHS-protein coupling. The dye is highly photostable as determined by single protein tracking on lipid bilayers (image of single proteins attached, scale bar is 5 micron). The only reason I give the product 4 stars instead of 5 is the packaging. The dye is shipped as a solid in a glass bottle with a flexible rubber cap. Most of the dye gets stuck inside and on the surface of this cap; thus not delivering a full value for money. I will highly recommend the manufacturers to use bottles with plastic caps so that the dye can be recovered a 100%

review image

Protocols for Janelia Fluor® 646, SE

The following protocol features additional information for the use of Janelia Fluor® 646, SE (Cat. No. 6148).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


New Product Guide

New Product Guide (Spring/Summer 2018)

Our new product guide highlights over 200 new products added to the Tocris Bioscience range during the second half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Aptamers
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells