Janelia Fluor® 646, SE

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Cat.No. 6148 - Janelia Fluor 646, SE | C33H31N3O6Si | CAS No. 1811539-59-9
Description: Cell permeable fluorescent dye; supplied as NHS ester
Alternative Names: JF 646, NHS
Chemical Name: 1-[7-(1-Azetidinyl)-10-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt
Datasheet
Citations
Literature

Biological Activity

Fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Compatible with self-labeling tag systems (e.g. HaloTag® and SNAPtag® ). Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and PAINT imaging. Can be multiplexed for two colour imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54, Extinction coefficient = 152,000 M-1cm-1; A280 correction factor is 0.19.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Application of Janelia Fluor® Dyes in Cardiac Tissue

Application of Janelia Fluor® Dyes in Cardiac Tissue

Super-resolution image (dSTORM) of muscle tissue from the left ventricle of an adult pig heart. JF646 (Cat.No. 6148) was used to label collagen VI, which is present on the cell plasma membrane, and imaged with a 642 nm excitation laser. A clearer structure is achieved in comparison with the widefield image (top right). Scale bar 2 μm.

Image kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Alex Clowsley and Anna Meletiou.

Technical Data

M. Wt 593.71
Formula C33H31N3O6Si
Storage Store at -20°C
CAS Number 1811539-59-9
PubChem ID 124201858
InChI Key ODBCGJUBBYOJOS-UHFFFAOYSA-N
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3[Si]5(C)C)=C(C=C/6)C5=CC6=[N+]7CCC\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 59.37 100

Preparing Stock Solutions

The following data is based on the product molecular weight 593.71. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.68 mL 8.42 mL 16.84 mL
5 mM 0.34 mL 1.68 mL 3.37 mL
10 mM 0.17 mL 0.84 mL 1.68 mL
50 mM 0.03 mL 0.17 mL 0.34 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Schmidt et al (2016) Live cell imaging reveals the dynamics of telomerase recruitment to telomeres. Cell 166 1188 PMID: 27523609

Li et al (2016) Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. Elife 5 e17056 PMID: 27484239

Legant et al (2016) High-density three-dimensional localization microscopy across large volumes. Nat.Methods 13 359 PMID: 26950745

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551

Deng et al (2015) CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc.Natl.Acad.Sci.USA. 112 11870 PMID: 26324940

Ticau et al (2015) Single-molecule studies of origin licensing reveal mechanisms ensuring bidirectional helicase loading. Cell 161 513 PMID: 25892223


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