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Glycosylases are a group of enzymes that includes glucosidases, mannosidases and heparanases. There are two glucosidase subtypes, both found in the gut. They hydrolyze terminal (1,4)α-glucosidic linkages and (1,6)β-glucosidic linkages, liberating α-glucose and β-glucose.
|Cat. No.||Product Name / Activity|
|Glucosidase α inhibitor (intestinal)|
|Glucosidases α and β inhibitor|
|7056||Conduritol B epoxide|
|Irreversible glucocerebrosidase (β-glucosidase) inhibitor; used to generate Gaucher disease models|
|α-Mannosidase I inhibitor|
|Glucosidase I and II inhibitor|
|Inhibitor of class I α-mannosidases|
|α-glucosidase I and II inhibitor; also inhibits ceramide-specific glycosyltransferases and exhibits broad spectrum antiviral activity|
|6236||O8 OGG1 Inhibitor|
|Potent and selective 8-oxoguanine DNA glycosylase 1 (OGG1) inhibitor|
|Heparanase inhibitor. Antiangiogenic|
|Inhibitor of α-mannosidase II|
|Selective 8-oxoguanine DNA glycosylase 1 (OGG1) inhibitor|
Glycosylases are a group of enzymes that includes glucosidases, mannosidases and heparanases. There are two glucosidase subtypes, α- and β-glucosidase (EC numbers 220.127.116.11 and 18.104.22.168 respectively), which are both found in the gut. They hydrolyze terminal (1,4)α-glucosidic linkages and (1,6)β-glucosidic linkages, liberating α-glucose and β-glucose respectively.
Mannosidases are divided into two subtypes: I and II, (EC numbers 22.214.171.124 and 126.96.36.199 respectively) which display a wide expression pattern. Mannosidase I hydrolyzes (1,2)-linked α-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2 and mannosidase II hydrolyzes (1,3)- and (1,6)-linked α-D-mannose residues in Man5(GlcNAc)3. Both subtypes require a divalent cation cofactor.
Heparanases are endo β-D-glucuronidase enzymes that cleave heparan sulfate side chains of heparan sulfate proteoglycans on cell surfaces and the extracellular matrix. Heparanases are involved in the clotting pathway and interact with tissue factor (TF) and TF pathway inhibitor (TFPI) to promote coagulation. Additional physiological roles include heparan sulfate turnover, embryo development, hair growth, and wound healing.
Inherited mutations in glucosidases can cause diseases such as Pompe disease (α-glucosidase deficiency) and mutations in mannosidases can cause mannosidosis (mannosidase I deficiency). Overexpression of heparanases is strongly implicated in tumor metastasis and angiogenesis.
|Gene||Species||Gene Symbol||Gene Accession No.||Protein Accession No.|
|Mannosidase I A1||Human||MAN1A1||NM_005907||P33908|
|Mannosidase I A2||Human||MAN1A2||NM_006699||O60476|
|Mannosidase I B1||Human||MAN1B1||NM_016219||Q9UKM7|
|Mannosidase I C1||Human||MAN1C1||NM_020379||Q9NR34|
|Mannosidase II A1||Human||MAN2A1||NM_002372||Q16706|
|Mannosidase II A2||Human||MAN2A2||NM_006122||P49641|
|Mannosidase II B1||Human||MAN2B1||NM_000528||O00754|
|Mannosidase II B2||Human||MAN2B2||NM_015274||Q9Y2E5|
|Mannosidase II C1||Human||MAN2C1||NM_006715||Q9NTJ4|