Glycosylases

Glycosylases are a group of enzymes that includes glucosidases, mannosidases and heparanases. There are two glucosidase subtypes, both found in the gut. They hydrolyze terminal (1,4)α-glucosidic linkages and (1,6)β-glucosidic linkages, liberating α-glucose and β-glucose.

Products
Background
Literature
Gene Data

Inhibitors

Cat No Product Name / Activity
2673 Acarbose
Glucosidase α inhibitor (intestinal)
0759 Castanospermine
Glucosidases α and β inhibitor
1259 1-Deoxymannojirimycin hydrochloride
α-Mannosidase I inhibitor
1258 1-Deoxynojirimycin
Glucosidase I and II inhibitor
3207 Kifunensine
Inhibitor of class I α-mannosidases
4985 Miglitol
α-glucosidase inhibitor
3117 Miglustat hydrochloride
α-glucosidase I and II inhibitor. Also inhibits ceramide-specific glycosyltransferases
3691 N-Nonyldeoxynojirimycin
Glucosidase inhibitor
2710 OGT 2115
Heparanase inhibitor. Antiangiogenic
3208 Swainsonine
Inhibitor of α-mannosidase II
3596 Voglibose
Orally active α-glucosidase inhibitor

Glycosylases are a group of enzymes that includes glucosidases, mannosidases and heparanases. There are two glucosidase subtypes, α- and β-glucosidase (EC numbers 3.2.1.20 and 3.2.1.21 respectively), which are both found in the gut. They hydrolyze terminal (1,4)α-glucosidic linkages and (1,6)β-glucosidic linkages, liberating α-glucose and β-glucose respectively.

Mannosidases are divided into two subtypes: I and II, (EC numbers 3.2.1.113 and 3.2.1.114 respectively) which display a wide expression pattern. Mannosidase I hydrolyzes (1,2)-linked α-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2 and mannosidase II hydrolyzes (1,3)- and (1,6)-linked α-D-mannose residues in Man5(GlcNAc)3. Both subtypes require a divalent cation cofactor.

Heparanases are endo β-D-glucuronidase enzymes that cleave heparan sulfate side chains of heparan sulfate proteoglycans on cell surfaces and the extracellular matrix. Heparanases are involved in the clotting pathway and interact with tissue factor (TF) and TF pathway inhibitor (TFPI) to promote coagulation. Additional physiological roles include heparan sulfate turnover, embryo development, hair growth, and wound healing.

Inherited mutations in glucosidases can cause diseases such as Pompe disease (α-glucosidase deficiency) and mutations in mannosidases can cause mannosidosis (mannosidase I deficiency). Overexpression of heparanases is strongly implicated in tumor metastasis and angiogenesis.

Literature for Glycosylases

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New Product Guide (Spring/Summer 2017)

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  • 7-TM Receptors
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Glycosylase Gene Data

Gene Species Gene Symbol Gene Accession No. Protein Accession No.
α-Glucosidase Human GAA NM_000152 P10253
Mouse Gaa NM_008064 P70699
Rat Gaa NM_199118 Q6P7A9
β-Glucosidase Human GBA NM_000157 P04062
Mouse Gba NM_008094 P17439
Rat Gba NM_001127639 NP_001121111
Mannosidase I A1 Human MAN1A1 NM_005907 P33908
Mouse Man1a NM_008548 P45700
Rat Man1a1 NM_001033656 Q5D005
Mannosidase I A2 Human MAN1A2 NM_006699 O60476
Rat Man1a2 NM_001106452 XP_227543
Mannosidase I B1 Human MAN1B1 NM_016219 Q9UKM7
Rat Man1b1 XM_575086 XP_575086
Mannosidase I C1 Human MAN1C1 NM_020379 Q9NR34
Rat Man1c1 NM_001108687 XP_342944
Mannosidase II A1 Human MAN2A1 NM_002372 Q16706
Mouse Man2a1 NM_008549 P27046
Rat Man2a1 NM_012979 P28494
Mannosidase II A2 Human MAN2A2 NM_006122 P49641
Mouse Man2a2 NM_172903 NP_766491
Rat Man2a2 NM_001107527 XP_218816
Mannosidase II B1 Human MAN2B1 NM_000528 O00754
Mouse Man2b1 NM_010764 O09159
Rat Man2b1 NM_199404 Q6P762
Mannosidase II B2 Human MAN2B2 NM_015274 Q9Y2E5
Mouse Man2b2 NM_008550 O54782
Rat Man2b2 NM_001134971 NP_001128443
Mannosidase II C1 Human MAN2C1 NM_006715 Q9NTJ4
Mouse Man2c1 NM_028636 Q91W89
Rat Man2c1 NM_139256 Q5M9I2
Heparanase Human HPSE NM_006665 Q9Y251
Mouse Hpse NM_152803 Q6YGZ1
Rat Hpse NM_022605 Q71RP1
Heparanase 2 Human HPSE2 NM_021828 Q8WWQ2
Mouse Hpse2 NM_001081257 NP_001074726
Rat Hpse2 NM_001135762 XP_347297