These Stem Cell Protocol snapshots are intended to highlight how small molecules may be used in place of or in combination with growth factors and other proteins in cell culture, reprogramming and differentiation. Using small molecules in stem cell protocols offers several advantages:
- their effects are rapid and reversible, making their use easy to control with time
- they are cell permeable so can be used to target intracellular pathways
- they are chemically synthesized, ensuring high purity, low batch-to-batch variability and reproducible results
- their activity is often concentration-dependent so they can be used in different protocols with different outcomes.