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Description: Inhibitor of hedgehog (Hh) signaling; antagonizes smoothened activity
Chemical Name: N-[(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)methylene]-4-(phenylmethyl)-1-piperazinamine
Purity: ≥98% (HPLC)
Citations (10)
Protocols (1)
Literature (4)

Biological Activity for SANT-1

SANT-1 is a potent, cell-permeable inhibitor of Sonic hedgehog (Shh) signaling; high affinity antagonist of smoothened activity (KD = 1.2 nM). Inhibits smoothened agonist effects with an IC50 of 20 nM (in Shh-LIGHT2 cells). Has been used in protocols to generate pancreatic β cells from hPSCs.

For more information about how SANT-1 may be used, see our protocol: Generation of β cells from hPSCs

Compound Libraries for SANT-1

SANT-1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for SANT-1

M. Wt 373.5
Formula C23H27N5
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 304909-07-7
PubChem ID 6878030
Smiles CC1=NN(C(C)=C1\C=N\N1CCN(CC2=CC=CC=C2)CC1)C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for SANT-1

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 18.68 50

Preparing Stock Solutions for SANT-1

The following data is based on the product molecular weight 373.5. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 5.35 mL 26.77 mL 53.55 mL
2.5 mM 1.07 mL 5.35 mL 10.71 mL
5 mM 0.54 mL 2.68 mL 5.35 mL
25 mM 0.11 mL 0.54 mL 1.07 mL

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References for SANT-1

References are publications that support the biological activity of the product.

Chen et al (2002) Small molecule modulation of smoothened activity. Proc.Natl.Acad.Sci.USA. 99 1407

If you know of a relevant reference for SANT-1, please let us know.

View Related Products by Product Action

View all Hedgehog Signaling Inhibitors

Keywords: SANT-1, SANT-1 supplier, inhibitors, inhibits, hedgehog, Hh, signaling, antagonizes, smoothened, activity, Signaling, Signalling, Smo, Receptors, antagonist, Hedgehog, Smoothened, Organoids, 1974, Tocris Bioscience

10 Citations for SANT-1

Citations are publications that use Tocris products. Selected citations for SANT-1 include:

Yuan et al (2016) Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation. Nat Commun 21 11024 PMID: 26996322

Nostro et al (2015) Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. Neuropsychopharmacology 4 591 PMID: 25843049

Sambathkumar et al (2018) Generation of hepatocyte- and endocrine pancreatic-like cells from human induced endodermal progenitor cells. PLoS One 13 e0197046 PMID: 29750821

Krentz et al (2017) Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors. Dev Cell 41 129 PMID: 28441528

Do you know of a great paper that uses SANT-1 from Tocris? Please let us know.

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Protocols for SANT-1

The following protocol features additional information for the use of SANT-1 (Cat. No. 1974).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Stem Cell Research Product Guide

Stem Cell Research Product Guide

This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:

  • Self-renewal and Maintenance
  • Differentiation
  • Reprogramming
  • Organoid Generation
  • GMP and Ancillary Material Grade Products
Stem Cells Scientific Review

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Stem Cell Workflow Poster

Stem Cell Workflow Poster

Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.

Stem Cells Poster

Stem Cells Poster

Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.