SANT-1

Pricing Availability   Qty
Cat.No. 1974 - SANT-1 | C23H27N5 | CAS No. 304909-07-7
Description: Inhibitor of hedgehog (Hh) signaling; antagonizes smoothened activity
Chemical Name: N-[(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)methylene]-4-(phenylmethyl)-1-piperazinamine
Purity: ≥99% (HPLC)
Datasheet
Citations (10)
Reviews
Protocols (1)
Literature (5)

Biological Activity

Potent, cell-permeable inhibitor of Sonic hedgehog (Shh) signaling; high affinity antagonist of smoothened activity (KD = 1.2 nM). Inhibits smoothened agonist effects with an IC50 of 20 nM (in Shh-LIGHT2 cells). Has been used in protocols to generate pancreatic β cells from hPSCs.

Compound Libraries

SANT-1 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 373.5
Formula C23H27N5
Storage Desiccate at +4°C
Purity ≥99% (HPLC)
CAS Number 304909-07-7
PubChem ID 6878030
InChI Key FOORCIAZMIWALX-JJIBRWJFSA-N
Smiles CC1=NN(C(C)=C1\C=N\N1CCN(CC2=CC=CC=C2)CC1)C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
ethanol 9.34 25
DMSO 18.68 50

Preparing Stock Solutions

The following data is based on the product molecular weight 373.5. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 5.35 mL 26.77 mL 53.55 mL
2.5 mM 1.07 mL 5.35 mL 10.71 mL
5 mM 0.54 mL 2.68 mL 5.35 mL
25 mM 0.11 mL 0.54 mL 1.07 mL

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References

References are publications that support the biological activity of the product.

Chen et al (2002) Small molecule modulation of smoothened activity. Proc.Natl.Acad.Sci.USA. 99 1407


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View Related Products by Product Action

View all Hedgehog Signaling Inhibitors

Keywords: SANT-1, SANT-1 supplier, inhibitors, inhibits, hedgehog, Hh, signaling, antagonizes, smoothened, activity, Signaling, Signalling, Smo, Receptors, antagonist, Hedgehog, Smoothened, 1974, Tocris Bioscience

10 Citations for SANT-1

Citations are publications that use Tocris products. Selected citations for SANT-1 include:

Yung et al (2019) Sufu- And Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals. Nat.Commun. 10 4647 PMID: 31604927

Fleury et al (2016) Hedgehog associated to microparticles inhibits adipocyte differentiation via a non-canonical pathway. Sci Rep 6 23479 PMID: 27010359

Wang et al (2012) Glucocorticoid compounds modify smoothened localization and hedgehog pathway activity. Chem Biol 19 972 PMID: 22921064

Strating et al (2015) Itraconazole inhibits enterovirus replication by targeting the oxysterol-binding protein. Cell Rep 10 600 PMID: 25640182


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Protocols for SANT-1

The following protocol features additional information for the use of SANT-1 (Cat. No. 1974).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


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