SANT-1

Pricing Availability Delivery Time Qty
Cat.No. 1974 - SANT-1 | C23H27N5 | CAS No. 304909-07-7
Description: Inhibitor of hedgehog (Hh) signaling; antagonizes smoothened activity
Chemical Name: N-[(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)methylene]-4-(phenylmethyl)-1-piperazinamine
Purity: ≥99% (HPLC)
Datasheet
Citations (2)
Literature

Biological Activity

Potent, cell-permeable inhibitor of Sonic hedgehog (Shh) signaling; high affinity antagonist of smoothened activity (KD = 1.2 nM). Inhibits smoothened agonist effects with an IC50 of 20 nM (in Shh-LIGHT2 cells).

Compound Libraries

SANT-1 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 373.5
Formula C23H27N5
Storage Desiccate at +4°C
Purity ≥99% (HPLC)
CAS Number 304909-07-7
PubChem ID 6878030
InChI Key FOORCIAZMIWALX-JJIBRWJFSA-N
Smiles CC1=NN(C(C)=C1\C=N\N1CCN(CC2=CC=CC=C2)CC1)C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 18.68 50
ethanol 9.34 25

Preparing Stock Solutions

The following data is based on the product molecular weight 373.5. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.68 mL 13.39 mL 26.77 mL
5 mM 0.54 mL 2.68 mL 5.35 mL
10 mM 0.27 mL 1.34 mL 2.68 mL
50 mM 0.05 mL 0.27 mL 0.54 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Chen et al (2002) Small molecule modulation of smoothened activity. Proc.Natl.Acad.Sci.USA. 99 1407 PMID:


If you know of a relevant reference for SANT-1, please let us know.

View Related Products by Product Action

View all Hedgehog Signaling Inhibitors

Keywords: SANT-1, supplier, inhibitors, inhibits, hedgehog, Hh, signaling, antagonizes, smoothened, activity, Signaling, Signalling, Smo, Receptors, antagonist, Hedgehog, Signaling, Tocris Bioscience

2 Citations for SANT-1

Citations are publications that use Tocris products. Selected citations for SANT-1 include:

Nostro et al (2015) Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. Neuropsychopharmacology 4 591 PMID: 25843049

Atwood et al (2013) GLI activation by atypical protein kinase C Ι/λ regulates the growth of basal cell carcinomas. Stem Cell Reports 494 484 PMID: 23446420


Do you know of a great paper that uses SANT-1 from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Stem Cells

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Pathways for SANT-1

Protocols

TODO: Add Protocols