ML 210

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Cat.No. 6429 - ML 210 | C22H20Cl2N4O4 | CAS No. 1360705-96-9
Description: Selectively kills mutant HRAS-expressing cells; glutathione peroxidase inhibitor; induces ferroptosis
Chemical Name: [4-[Bis(4-chlorophenyl)methyl]-1-piperazinyl](5-methyl-4-nitro-3-isoxazolyl)methanone
Purity: ≥98% (HPLC)
Literature (4)

Biological Activity

Glutathione peroxidase (GPX4) inhibitor. Kills mutant RAS-expressing cell lines (IC50 values are 71 and 272 nM for HRAS G12v mutant expressing cell lines BJeLR and BJeH-LT, respectively). Exhibits 4-fold selectivity for HRAS mutant-expressing cell lines. Induces ferroptosis in tumor initiation "persister" cells and drug-resistant tumor cells.

Compound Libraries

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Technical Data

M. Wt 475.32
Formula C22H20Cl2N4O4
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1360705-96-9
PubChem ID 49766530
Smiles CC1=C([N+]([O-])=O)C(C(N2CCN(C(C3=CC=C(C=C3)Cl)C4=CC=C(C=C4)Cl)CC2)=O)=NO1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 23.77 50

Preparing Stock Solutions

The following data is based on the product molecular weight 475.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 4.21 mL 21.04 mL 42.08 mL
2.5 mM 0.84 mL 4.21 mL 8.42 mL
5 mM 0.42 mL 2.1 mL 4.21 mL
25 mM 0.08 mL 0.42 mL 0.84 mL

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References are publications that support the biological activity of the product.

Weïwer et al (2012) Development of small-molecule probes that selectively kill cells induced to express mutant RAS. Bioorg.Med.Chem.Lett. 22 1822 PMID: 22297109

Yang et al (2014) Regulation of ferroptotic cancer cell death by GPX4. Cell 156 317 PMID: 24439385

Hangauer et al (2017) Drug-tolerant persister cancer cells are vulnerable to GPX4 inhibition. Nature 551 247 PMID: 29088702

If you know of a relevant reference for ML 210, please let us know.

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Literature in this Area

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