Inhibitor of Wnt processing and secretion. Inactivates PORCN, a membrane-bound O-acyltransferase (MBOAT), and selectively inhibits palmitoylation of Wnt. Blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. Suppresses self-renewal in R1 embryonic stem cells.
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
|DMSO||2.33||5 with gentle warming|
Preparing Stock Solutions
The following data is based on the product molecular weight 466.6. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.05 mM||42.86 mL||214.32 mL||428.63 mL|
|0.25 mM||8.57 mL||42.86 mL||85.73 mL|
|0.5 mM||4.29 mL||21.43 mL||42.86 mL|
|2.5 mM||0.86 mL||4.29 mL||8.57 mL|
References are publications that support the biological activity of the product.
Chen et al (2009) Small molecule-mediated disruption of wnt-dependent signaling in tissue regeneration and cancer. Nature Chem.Biol. 5 100 PMID: 19125156
ten Berge et al (2011) Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells. Nat.Cell.Biol. 13 1070 PMID: 21841791
Lian et al (2012) Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc.Natl.Acad.Sci.U S A 109 E1848 PMID: 22645348
If you know of a relevant reference for IWP 2, please let us know.
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Keywords: IWP 2, IWP 2 supplier, MBOAT, membrane-bound, o-acyltransferases, Porcupine, porcn, wnt, secretion, production, signaling, signalling, inhibitors, inhibits, inactivators, inactivates, IWP2, beta-catenin, β-catenin, b-catenin, stem, cell, proliferation, Stem, Cell, Signaling, Proliferation, PORCN, 3533, Tocris Bioscience
19 Citations for IWP 2
Citations are publications that use Tocris products. Selected citations for IWP 2 include:
Magro-Lopez et al (2018) Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids. Stem Cell Res.Ther. 9 186 PMID: 29996941
Walko et al (2017) A genome-wide screen identifies YAP/WBP2 interplay conferring growth advantage on human epidermal stem cells. Nat Commun 8 14744 PMID: 28332498
Lee et al (2017) Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations. Cell Stem Cell 21 179 PMID: 28777944
Tong (2017) Degree of tissue differentiation dictates susceptibility to BRAF-driven colorectal cancer. Cell Rep 21 3833 PMID: 29281831
Huang et al (2015) The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells. Sci Rep 10 413 PMID: 25654758
Lee et al (2015) A systems-biological study on the identification of safe and effective molecular targets for the reduction of ultraviolet B-induced skin pigmentation. Nat Commun 5 10305 PMID: 25980672
Freedman et al (2015) Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids. Nat Cell Biol 6 8715 PMID: 26493500
Ditadi et al (2015) Human definitive haemogenic endothelium and arterial vascular endothelium represent distinct lineages. Stem Cell Res 17 580 PMID: 25915127
Kempf et al (2014) Controlling expansion and cardiomyogenic differentiation of human pluripotent stem cells in scalable suspension culture. Stem Cell Reports 3 1132 PMID: 25454631
Huang et al (2014) Efficient generation of lung and airway epithelial cells from human pluripotent stem cells. Nat Biotechnol 32 84 PMID: 24291815
Kennedy et al (2016) Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription. Proc Natl Acad Sci U S A 113 3545 PMID: 26969725
Hojo et al (2013) Hedgehog-Gli activators direct osteo-chondrogenic function of bone morphogenetic protein toward osteogenesis in the perichondrium. J Biol Chem 288 9924 PMID: 23423383
Kumar et al (2015) Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells. Elife 4 PMID: 26554899
Kim et al (2015) Activin-A and Bmp4 levels modulate cell type specification during CHIR-induced cardiomyogenesis. PLoS One 10 e0118670 PMID: 25706534
Heylman et al (2015) Supervised Machine Learning for Classification of the Electrophysiological Effects of Chronotropic Drugs on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. PLoS One 10 e0144572 PMID: 26695765
Huebsch et al (2015) Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales. Tissue Eng Part C Methods 21 467 PMID: 25333967
Lee et al (2015) Machine learning plus optical flow: a simple and sensitive method to detect cardioactive drugs. Sci Rep 5 11817 PMID: 26139150
Skelton et al (2014) SIRPA, VCAM1 and CD34 identify discrete lineages during early human cardiovascular development. Nat Genet 13 172 PMID: 24968096
Faisy et al (2014) Wnt/β-catenin signaling modulates human airway sensitization induced by β2-adrenoceptor stimulation. PLoS One 9 e111350 PMID: 25360795
Do you know of a great paper that uses IWP 2 from Tocris? Please let us know.
Reviews for IWP 2
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I use it as a blocking molecule of the WNT pathway for cardiac differentiation in foreskin fibroblast-derive IPS cells. I obtained succesfully cardiomyocytes in a 5uM concentration.
Literature in this Area
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