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Description: Ultrabright fluorogenic dye for imaging RNA in living cells; activated by binding Mango aptamers
Chemical Name: (E)-3-(2-((2-Fluoro-6-(1H-pyrazol-1-yl)benzyl)amino)-2-oxoethyl)-2-((1-methylquinolin-4(1H)-ylidene)methyl)benzo[d]thiazol-3-ium trifluoroacetate
Purity: ≥95% (HPLC)
Biological Activity for SALAD1
Key information: SALAD1 is a specific and high-affinity aptamer-ligand for aptamer Mango II (KDapp = 0.69 nM). Green fluorogenic dye displaying 3.5-fold brighter fluorescence than other Mango aptamer dyes such as TO1-biotin. Binds 7.5-fold more tightly to Mango II compared to TO dye. SALAD1 exhibits a binding preference to its target RNA (Mango II) over the other three Mango aptamers (7-, 20- and 16-fold tighter than Mango I, III and IV).
The improved brightness and tighter binding of SALAD1 to Mango aptamers allow detection and tracking of RNA with greater sensitivity and spatial resolution, facilitating studies of RNA localization, dynamics, and expression in live-cell and single-molecule imaging applications.
Used for: imaging RNA in live and fixed cells, even at low concentration.
Application: confocal cellular imaging.
Properties and Photophysical Data: excitation and emission maxima (λ) measured in Mango selection buffer are 511 nm and 540 nm, respectively; quantum yield QY = 0.722; extinction coefficient ε(complex) = 47,284 M-1cm-1; fluorescence enhancement FE = 1,500; fluorescence efficiency E = 2,170 nM-1; brightness = 34,139 M-1cm-1. SALAD1 is cell-permeable.
Optical Data for SALAD1
| Emission Color | Green |
|---|---|
| λabs | 511 nm |
| λem | 540 nm |
| Extinction Coefficient (ε) | 47,284 M-1cm-1 |
| Quantum Yield (φ) | 0.722 |
| Cell Permeable | Yes |
| Application | Confocal cellular imaging |
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for SALAD1
| M. Wt | 635.64 |
| Formula | C30H25FN5OS.C2F3O2 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| InChI Key | WDAIBHZPVVKNPB-UHFFFAOYSA-N |
| Smiles | CN1C=C/C(C2=C1C=CC=C2)=C\C3=[N+](C4=CC=CC=C4S3)CC(NCC5=C(N6C=CC=N6)C=CC=C5F)=O.O=C(C(F)(F)F)[O-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for SALAD1
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.36 | 10 |
Preparing Stock Solutions for SALAD1
The following data is based on the product molecular weight 635.64. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 15.73 mL | 78.66 mL | 157.32 mL |
| 0.5 mM | 3.15 mL | 15.73 mL | 31.46 mL |
| 1 mM | 1.57 mL | 7.87 mL | 15.73 mL |
| 5 mM | 0.31 mL | 1.57 mL | 3.15 mL |
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Product Datasheets for SALAD1
Certificate of Analysis is currently unavailable on-line.
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References for SALAD1
References are publications that support the biological activity of the product.
Yang et al (2025) Structure-informed design of an ultrabright RNA-activated fluorophore. Nat.Chem. 17 1188 PMID: 40437193
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals
- Tissue Clearing Kits and Reagents