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Description: Sensitive and cell-permeable fluorogenic probe for the detection of labile Fe(II) in live cells; suitable for high-throughput assays
Alternative Names: RhoNox-4
Chemical Name: 4-(tert-Butoxycarbonyl)-1-(3'-(4-(tert-butoxycarbonyl)piperazin-1-yl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-6'-yl)piperazine 1-oxide
Purity: ≥90% (HPLC)
Biological Activity for FerroOrange
Key information: FerroOrange (RhoNox-4) is a highly sensitive and robust fluorogenic probe for the detection of intracellular labile Fe(II). FerroOrange displays weak absorption and low fluorescence in the visible region, but becomes strongly fluorescent upon reaction with Fe(II).
Used for: detection of intracellular labile Fe(II).
Application: high-throughput assays for high content imaging of subcellular labile Fe(II) in live cells; fluorescent microscopy; studies on iron homeostasis and ferroptosis.
Properties and Photophysical Data: FerroOrange has excellent cell-membrane permeability and low cell toxicity. FerroOrange has no chelating ability. For the fluorescent form, excitation and emission maxima (λ) measured in 50 mM HEPES buffer (pH 7.4, 0.2% DMSO) are 545 nm and 575 nm, respectively; extinction coefficient ε = 73,500 M-1cm-1; quantum yield φ = 0.19.
Optical Data for FerroOrange
| Emission Color | Orange |
|---|---|
| λabs | 545 nm |
| λem | 575 nm |
| Extinction Coefficient (ε) | 73500 M-1cm-1 |
| Quantum Yield (φ) | 0.19 |
| Cell Permeable | Yes |
| Application | High-throughput assays for the detection of intracellular labile Fe(II) |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for FerroOrange
| M. Wt | 684.78 |
| Formula | C38H44N4O8 |
| Storage | Store at +4°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 2711762-70-6 |
| PubChem ID | 172913725 |
| InChI Key | BPPNJBCDCLJSAA-UHFFFAOYSA-N |
| Smiles | O=C1OC2(C3=C1C=CC=C3)C4=CC=C(N5CCN(CC5)C(OC(C)(C)C)=O)C=C4OC6=CC([N+]7(CCN(CC7)C(OC(C)(C)C)=O)[O-])=CC=C62 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for FerroOrange
References for FerroOrange
References are publications that support the biological activity of the product.
Hirayama et al (2020) High-throughput screening for the discovery of iron homeostasis modulators using an extremely sensitive fluorescent probe. Sensors 5 2950 PMID: 32885952
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
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