Potent and selective, ATP-competitive glycogen synthase kinase-3 (GSK-3) inhibitor (IC50 values are 5, 83, 300, 320 and > 10 000 nM at GSK-3, CDK5/p25, CDK2/cyclin A, CDK1/cyclin B and other common kinases respectively). Maintains self-renewal and pluripotency in embryonic stem cells via activation of the Wnt signaling pathway in vitro; also promotes proliferation and dedifferentiation in cardiomyocytes.
Negative Control also available.
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 356.17. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.81 mL||14.04 mL||28.08 mL|
|5 mM||0.56 mL||2.81 mL||5.62 mL|
|10 mM||0.28 mL||1.4 mL||2.81 mL|
|50 mM||0.06 mL||0.28 mL||0.56 mL|
References are publications that support the products' biological activity.
Meijer et al (2003) GSK-3-selective inhibitors derived from tyrian purple indirubins. Chem.Biol. 10 1255 PMID: 14700633
Polychronopoulos et al (2004) Structural basis for the synthesis of indirubins as potent and selective inhibtors of glycogen synthase kinase-3 and cyclin dependent kinases. J.Med.Chem. 47 935 PMID: 14761195
Tseng et al (2006) The GSK-3 inhibitor BIO promotes proliferation in mammalian cardiomyocytes. Chem.Biol. 13 957 PMID: 16984885
If you know of a relevant reference for BIO, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: BIO, supplier, Potent, selective, GSK-3, inhibitors, inhibits, Glycogen, Synthase, Kinases, 3, Carbohydrate, Metabolism, stem, cells, 6BIO, Glycogen, Synthase, Kinase, 3, Glycogen, Synthase, Kinase, Stem, Cell, Proliferation, Stem, Cell, Signaling, Glycogen, Synthase, Kinase, 3, Tocris Bioscience
10 Citations for BIO
Citations are publications that use Tocris products. Selected citations for BIO include:
Guo et al (2012) Slug and Sox9 cooperatively determine the mammary stem cell state. World J Gastroenterol 148 1015 PMID: 22385965
Berger et al (2014) PAK-dependent STAT5 serine phosphorylation is required for BCR-ABL-induced leukemogenesis. Cell Cycle 28 629 PMID: 24263804
Lu et al (2014) Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/β-catenin pathway. Sci Rep 5 5368 PMID: 25371059
Zhang et al (2015) GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis. PLoS Biol 13 e1002129 PMID: 25860027
Penas et al (2015) GSK3 inhibitors stabilize Wee1 and reduce cerebellar granule cell progenitor proliferation. Nat Commun 14 417 PMID: 25616418
Rhim et al (2015) A High-content screen identifies compounds promoting the neuronal differentiation and the midbrain dopamine neuron specification of human neural progenitor cells. Elife 5 16237 PMID: 26542303
Lindström et al (2015) Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron. Nat Chem Biol 3 e04000 PMID: 25647637
Thorne et al (2015) GSK-3 modulates cellular responses to a broad spectrum of kinase inhibitors. Stem Cells Transl Med 11 58 PMID: 25402767
Efthymiou et al (2015) Rescue of an in vitro neuron phenotype identified in Niemann-Pick disease, type C1 induced pluripotent stem cell-derived neurons by modulating the WNT pathway and calcium signaling. Elife 4 230 PMID: 25637190
Pronobis et al (2015) A novel GSK3-regulated APC:Axin interaction regulates Wnt signaling by driving a catalytic cycle of efficient βcatenin destruction. Cell 4 e08022 PMID: 26393419
Do you know of a great paper that uses BIO from Tocris? If so please let us know.
Reviews for BIO
There are currently no reviews for this product. Be the first to review BIO and earn rewards!
Have you used BIO?
Submit a review and receive an Amazon gift card.
$10US/$10CAN/€7/£6 gift card for a review without an image
$25US/$25CAN/€18/£15 gift card for a review with an image
*Offer only valid in the USA / Canada, UK and EuropeSubmit a Review
Literature in this Area
Alzheimer's disease (AD) is a degenerative brain disease and the most common cause of dementia, affecting approximately 47 million people worldwide. Updated in 2015, this poster summarizes the structural and functional changes observed in the progression of this neurodegenerative disease, as well as classic AD drug targets.
Schizophrenia is a debilitating psychiatric disorder that affects 1% of the worldwide population. This poster describes the neurobiology of Schizophrenia, as well as highlighting the genetic and environmental factors that play a fundamental role in the etiology of the disease. The current and emerging drug targets are also discussed.