Neuronal nitric oxide synthase (nNOS), with inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS), catalyzes nitric oxide and L-citrulline generation from L-arginine and molecular oxygen. nNOS is activated at calcium concentrations over 100 nM.

Literature (1)
Gene Data

nNOS Inhibitors

Cat. No. Product Name / Activity
3319 ARL 17477 dihydrochloride
Selective nNOS inhibitor
0735 3-Bromo-7-nitroindazole
Selective nNOS inhibitor
0546 L-NIO dihydrochloride
NOS inhibitor


Cat. No. Product Name / Activity
7897 Sakura 6
Promoter of SERT and nNOS interaction
7898 ZZL 7
Inhibitor of SERT and nNOS interaction

Neuronal nitric oxide synthase (nNOS), along with inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS), catalyze the generation of nitric oxide and L-citrulline from L-arginine and molecular oxygen. It is activated at concentrations of calcium greater than 100 nM and requires co-factors of tetrahydrobiopterin, flavin adenine dinucleotide, flavin mononucleotide and NADPH for catalytic activity.

Transcriptional regulation of nNOS is very complex and the nNOS gene produces multiple mRNA transcripts using alternate promoters, alternative splicing, cassette insertions/deletions, and varied sites for 3'-UTR cleavage and polyadenylation. Some of these alterations lead to variation in amino acid sequence, translating into nNOS isoforms with altered structural and functional properties.

nNOS has been implicated in a wide variety of physiological and pathological processes, including neurotransmission, neurotoxicity, skeletal muscle contraction, sexual function, body fluid homeostasis and atherosclerosis. Consistent with such wide involvement, nNOS is expressed in numerous tissues. The human gene encoding nNOS is localized to chromosome 12q14.

External sources of pharmacological information for nNOS :

Literature for nNOS

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nNOS Gene Data

Species Gene Symbol Gene Accession No. Protein Accession No.
Human NOS1 NM_000620 P29475
Mouse Nos1 NM_008712 Q9Z0J4
Rat Nos1 NM_052799 P29476