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Inhibitor of cholesterol synthesis and cellular transport, and weak inhibitor of hedgehog (Hh) signaling. Reduces serum sterol levels in rats in vivo.
|Storage||Desiccate at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 424.07. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.36 mL||11.79 mL||23.58 mL|
|5 mM||0.47 mL||2.36 mL||4.72 mL|
|10 mM||0.24 mL||1.18 mL||2.36 mL|
|50 mM||0.05 mL||0.24 mL||0.47 mL|
References are publications that support the biological activity of the product.
Cenedella et al (1980) Concentration-dependent effects of AY-9944 and U18666A on sterol synthesis in brain. Biochem.Pharmacol. 29 2751 PMID: 6159896
Incardona et al (2000) Cyclopamine inhibition of sonic hedgehog signal transduction is not mediated through effects on cholesterol transport. Dev.Biol. 224 440 PMID: 10926779
Liscum and Faust (1989) The intracellular transport of low density lipoprotein-derived cholesterol is inhibited in Chinese hamster ovary cells cultured with 3-β-[2-(diethylamino)ethoxy]androst-5-en-17-one. J.Biol.Chem. 264 11796 PMID: 2745416
If you know of a relevant reference for U 18666A, please let us know.
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Keywords: U 18666A, U 18666A supplier, inhibitors, hedgehog, Hh, signaling, inhibits, cholesterol, synthesis, Signalling, Hedgehog, U18666A, Signaling, 1638, Tocris Bioscience
Citations for U 18666A
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Reviews for U 18666A
Average Rating: 5 (Based on 1 Review.)
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I used 2.5-10 ug/ml of U18666A to inhibit the cholesterol efflux in HeLa cells and it worked perfectly as I observed in U18666A treated HeLa cells accumulated cholesterol significantly. I used Filipin staining to image and quantitate the cholesterol levels.
I dissolved the U18666A in water and stored at -20C in aliquots.
Literature in this Area
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Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.