You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!Submit Review
Inhibitor of cholesterol synthesis and cellular transport, and weak inhibitor of hedgehog (Hh) signaling. Reduces serum sterol levels in rats in vivo.
|Storage||Desiccate at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 424.07. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.36 mL||11.79 mL||23.58 mL|
|5 mM||0.47 mL||2.36 mL||4.72 mL|
|10 mM||0.24 mL||1.18 mL||2.36 mL|
|50 mM||0.05 mL||0.24 mL||0.47 mL|
References are publications that support the biological activity of the product.
Cenedella et al (1980) Concentration-dependent effects of AY-9944 and U18666A on sterol synthesis in brain. Biochem.Pharmacol. 29 2751 PMID: 6159896
Incardona et al (2000) Cyclopamine inhibition of sonic hedgehog signal transduction is not mediated through effects on cholesterol transport. Dev.Biol. 224 440 PMID: 10926779
Liscum and Faust (1989) The intracellular transport of low density lipoprotein-derived cholesterol is inhibited in Chinese hamster ovary cells cultured with 3-β-[2-(diethylamino)ethoxy]androst-5-en-17-one. J.Biol.Chem. 264 11796 PMID: 2745416
If you know of a relevant reference for U 18666A, please let us know.
View Related Products by Product Action
Keywords: U 18666A, U 18666A supplier, inhibitors, hedgehog, Hh, signaling, inhibits, cholesterol, synthesis, Signalling, Hedgehog, U18666A, Signaling, 1638, Tocris Bioscience
Citations for U 18666A
Citations are publications that use Tocris products.
Currently there are no citations for U 18666A. Do you know of a great paper that uses U 18666A from Tocris? Please let us know.
Reviews for U 18666A
Average Rating: 5 (Based on 1 Review.)
Have you used U 18666A?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
I used 2.5-10 ug/ml of U18666A to inhibit the cholesterol efflux in HeLa cells and it worked perfectly as I observed in U18666A treated HeLa cells accumulated cholesterol significantly. I used Filipin staining to image and quantitate the cholesterol levels.
I dissolved the U18666A in water and stored at -20C in aliquots.
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.