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F 127 (20% solution in DMSO)
Pluronic is a registered trademark of BASF
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solubility||Soluble in DMSO (supplied pre-dissolved in DMSO (20%w/v)|
References are publications that support the biological activity of the product.
Poenie et al (1986) Calcium rises abruptly and briefly throughout the cell at the onset of anaphase. Science 233 886 PMID: 3755550
Drummond et al (1987) Depletion of intracellular calcium stores by calcium ionophore A23187 induces the genes for glucose-regulated proteins in hamster fibroblasts. J.Biol.Chem. 262 12801 PMID: 3691669
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HL-1 cells were uniformly loaded with Fura-2AM (1 µM), without compartmentalization, in presence of F127 (0.01%) in normal tyrode solution for 40 min.
Primary guinea pig ventricular cardiomyocytes were loaded with FURA-2AM (1 μM) and Pluronic F 127 (1%) for 30 min followed by washing with normal-Tyrode solution. Cells were stimulated at 1 Hz frequency and fluorescence intensities were measured. Loading of FURA-2AM is better in the presence of Pluronic F 127.