F 127 (20% solution in DMSO)

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Description: Nonionic detergent
Alternative Names: Pluronic® F 127 (20% solution in DMSO)
Reviews (2)

Biological Activity for F 127 (20% solution in DMSO)

F 127 (20% solution in DMSO) is a nonionic detergent used to solubilize large dye molecules and in particular AM esters including BAPTA AM (Cat. No. 2787), and FURA AM (Cat. No. 2220) and ci-IP3/PM (Cat. No. 6210).

Pluronic is a registered trademark of BASF

Technical Data for F 127 (20% solution in DMSO)

Storage Store at RT
CAS Number 9003-11-6
PubChem ID 24751

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for F 127 (20% solution in DMSO)

Solubility Soluble in DMSO (supplied pre-dissolved in DMSO (20%w/v)

Product Datasheets for F 127 (20% solution in DMSO)

Certificate of Analysis / Product Datasheet
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References for F 127 (20% solution in DMSO)

References are publications that support the biological activity of the product.

Poenie et al (1986) Calcium rises abruptly and briefly throughout the cell at the onset of anaphase. Science 233 886 PMID: 3755550

Drummond et al (1987) Depletion of intracellular calcium stores by calcium ionophore A23187 induces the genes for glucose-regulated proteins in hamster fibroblasts. J.Biol.Chem. 262 12801 PMID: 3691669

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Citations for F 127 (20% solution in DMSO)

Citations are publications that use Tocris products.

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Reviews for F 127 (20% solution in DMSO)

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F 127 facilitates uniform Fura2 loading in HL1 cells for Ca2+ imaging..
By Anonymous on 12/21/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: HL-1 Atrial Muscle Cell Line

HL-1 cells were uniformly loaded with Fura-2AM (1 µM), without compartmentalization, in presence of F127 (0.01%) in normal tyrode solution for 40 min.

Pluronic F 127 enhances loading of FURA-2AM in cardiomyocytes.
By Christopher Trummer on 10/15/2018
Assay Type: In Vitro
Species: Other
Cell Line/Tissue: primary ventricular cardiomyocytes

Primary guinea pig ventricular cardiomyocytes were loaded with FURA-2AM (1 μM) and Pluronic F 127 (1%) for 30 min followed by washing with normal-Tyrode solution. Cells were stimulated at 1 Hz frequency and fluorescence intensities were measured. Loading of FURA-2AM is better in the presence of Pluronic F 127.

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