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Biotin-PEG4-Diazirine

Cat. No. 8994 Submit a Review Datasheet / COA / SDS
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Description: Photo-crosslinking capture probe for deazaflavin-diazirine energy-transfer labelling (DarT labelling) with a biotin handle
Chemical Name: 1-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)-N-(4-(3-(trifluoromethyl)-3H-diazirin-3-yl)benzyl)-3,6,9,12-tetraoxapentadecan-15-amide
Purity: ≥95% (HPLC)
Datasheet
Citations
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Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Biotin-PEG4-Diazirine

Key information:Biotin-PEG4-Diazirine is a cell-permeable photo-crosslinking probe for elucidating protein interactome of polyarginine cell-penetrating peptides and mapping cell surfaceome through deazaflavin-diazirine energy-transfer labelling (DarT labelling). Upon UV irradiation and in the presence of deazaflavin as an organic photocatalyst, the diazirine group is activated to a reactive carbene that covalently crosslinks to nearby proteins. The appended biotin handle allows for efficient affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures such as cell lysates. Suitable for live-cell applications.

Used for: photocatalytic proximity labelling.

Application: deazaflavin-diazirine energy-transfer labelling (DarT labelling) followed by affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures.

Technical Data for Biotin-PEG4-Diazirine

M. Wt 688.75
Formula C30H43F3N6O7S
Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key MGUZAQIDWYWQHB-DPZBCOQUSA-N
Smiles O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(NCCOCCOCCOCCOCCC(NCC3=CC=C(C4(N=N4)C(F)(F)F)C=C3)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Biotin-PEG4-Diazirine

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 34.44 50 with gentle warming

Preparing Stock Solutions for Biotin-PEG4-Diazirine

The following data is based on the product molecular weight 688.75. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 2.9 mL 14.52 mL 29.04 mL
2.5 mM 0.58 mL 2.9 mL 5.81 mL
5 mM 0.29 mL 1.45 mL 2.9 mL
25 mM 0.06 mL 0.29 mL 0.58 mL

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Product Datasheets for Biotin-PEG4-Diazirine

Certificate of Analysis / Product Datasheet
Safety Datasheet

References for Biotin-PEG4-Diazirine

References are publications that support the biological activity of the product.

Crocker et al (2025) Energy-transfer photoproximity labelling in live cells using an organic cofactor. Nature Chemistry 17 1928 PMID: 40962911

If you know of a relevant reference for Biotin-PEG4-Diazirine, please let us know.

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Citations for Biotin-PEG4-Diazirine

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