FURA-2AM

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Cat.No. 2220 - FURA-2AM | C44H47N3O24 | CAS No. 108964-32-5
Description: Fluorescent Ca2+ indicator
Chemical Name: 2-[6-[bis[2-[(Acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid (acetyloxy)methyl ester
Purity: ≥95% (HPLC)
Datasheet
Citations (2)
Reviews (1)
Literature

Biological Activity

Fluorescent Ca2+ indicator. Selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. Used to determine intracellular Ca2+ concentration.

Technical Data

M. Wt 1001.85
Formula C44H47N3O24
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 108964-32-5
PubChem ID 90488749
InChI Key JTDRWQMCMPIBNJ-UHFFFAOYSA-N
Smiles CC1=CC(OCCOC2=C(N(CC(OCOC(C)=O)=O)CC(OCOC=O)=O)C=C(OC(C4=NC=C(C(OCOC(C)=O)=O)O4)=C3)C3=C2)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 10.02 10

Preparing Stock Solutions

The following data is based on the product molecular weight 1001.85. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1 mL 4.99 mL 9.98 mL
5 mM 0.2 mL 1 mL 2 mL
10 mM 0.1 mL 0.5 mL 1 mL
50 mM 0.02 mL 0.1 mL 0.2 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the biological activity of the product.

Grynkiewicz et al (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J.Biol.Chem. 260 3440 PMID: 3838314

Wang et al (2008) Sildenafil inhibits human pulmonary artery smooth muscle cell proliferation by decreasing capacitative Ca2+ entry. J.Pharmacol.Sci. 108 71 PMID: 18818482


If you know of a relevant reference for FURA-2AM, please let us know.

Keywords: FURA-2AM, FURA-2AM supplier, Fluorescent, Ca2+, indicator, Probes, Calcium, Signaling, Signalling, Agents, General, 2220, Tocris Bioscience

2 Citations for FURA-2AM

Citations are publications that use Tocris products. Selected citations for FURA-2AM include:

Luo et al (2015) Ketamine attenuates the Na+-dependent Ca2+ overload in rabbit ventricular myocytes in vitro by inhibiting late Na+ and L-type Ca2+ currents. Mol Neurodegener 36 1327 PMID: 26456586

Bayliss and Evans (2013) Characterisation of AmphiAmR11, an amphioxus (Branchiostoma floridae) D2-dopamine-like G protein-coupled receptor. PLoS One 8 e80833 PMID: 24265838


Do you know of a great paper that uses FURA-2AM from Tocris? Please let us know.

Reviews for FURA-2AM

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Detects cytosolic calcium influx changes well
By Anonymous on 11/01/2017
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: spinal cord neurons

Used in live cell calcium imaging assay to study glutamate receptor signaling in neurons. Used at a concentration of 5 micromolar (along with pluronic). Images shows changes in fura-2 fluorescence pre (left) and post(right) glutamate stimulation.

review image

Literature in this Area

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