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Fluorescent Ca2+ indicator. Selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. Used to determine intracellular Ca2+ concentration.
F 127 (Cat. No. 6253) for the solubilization of FURA-2AM is also available.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 1001.85. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||9.98 mL||49.91 mL||99.82 mL|
|0.5 mM||2 mL||9.98 mL||19.96 mL|
|1 mM||1 mL||4.99 mL||9.98 mL|
|5 mM||0.2 mL||1 mL||2 mL|
References are publications that support the biological activity of the product.
Grynkiewicz et al (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J.Biol.Chem. 260 3440 PMID: 3838314
Wang et al (2008) Sildenafil inhibits human pulmonary artery smooth muscle cell proliferation by decreasing capacitative Ca2+ entry. J.Pharmacol.Sci. 108 71 PMID: 18818482
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Keywords: FURA-2AM, FURA-2AM supplier, Fluorescent, Ca2+, indicator, Probes, Calcium, Signaling, Signalling, Agents, General, Ion, Indicators, and, Indicator, Dyes, 2220, Tocris Bioscience
3 Citations for FURA-2AM
Citations are publications that use Tocris products. Selected citations for FURA-2AM include:
Cabrita et al (2019) Niclosamide repurposed for the treatment of inflammatory airway disease. JCI Insight 4 PMID: 31391337
Bayliss and Evans (2013) Characterisation of AmphiAmR11, an amphioxus (Branchiostoma floridae) D2-DA-like G protein-coupled receptor. PLoS One 8 e80833 PMID: 24265838
Luo et al (2015) KA attenuates the Na+-dependent Ca2+ overload in rabbit ventricular myocytes in vitro by inhibiting late Na+ and L-type Ca2+ currents. Mol Neurodegener 36 1327 PMID: 26456586
Do you know of a great paper that uses FURA-2AM from Tocris? Please let us know.
Reviews for FURA-2AM
Average Rating: 4.7 (Based on 3 Reviews.)
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RBL-2H3 cells at a confluency of 50–60% were cultured on glass coverslip and loaded with 2 μM Fura-2AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. The Fura-2 loaded cells were excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm every 1.2 s. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.
Primary guinea pig ventricular cardiomyocytes were loaded with FURA-2AM (1 μM) and Pluronic F 127 (1%) for 30 min followed by washing with normal-Tyrode solution. Cells were stimulated at 1 Hz frequency and fluorescence intensities were measured.
Used in live cell calcium imaging assay to study glutamate receptor signaling in neurons. Used at a concentration of 5 micromolar (along with pluronic). Images shows changes in fura-2 fluorescence pre (left) and post(right) glutamate stimulation.
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