FURA-2AM

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Cat.No. 2220 - FURA-2AM | C44H47N3O24 | CAS No. 108964-32-5
Description: Fluorescent Ca2+ indicator
Chemical Name: 2-[6-[bis[2-[(Acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid (acetyloxy)methyl ester
Purity: ≥95% (HPLC)
Datasheet
Citations (3)
Reviews (3)
Literature (1)

Biological Activity

Fluorescent Ca2+ indicator. Selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. Used to determine intracellular Ca2+ concentration.

F 127 (Cat. No. 6253) for the solubilization of FURA-2AM is also available.

Technical Data

M. Wt 1001.85
Formula C44H47N3O24
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 108964-32-5
PubChem ID 90488749
InChI Key JTDRWQMCMPIBNJ-UHFFFAOYSA-N
Smiles CC1=CC(OCCOC2=C(N(CC(OCOC(C)=O)=O)CC(OCOC=O)=O)C=C(OC(C4=NC=C(C(OCOC(C)=O)=O)O4)=C3)C3=C2)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 10.02 10

Preparing Stock Solutions

The following data is based on the product molecular weight 1001.85. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 9.98 mL 49.91 mL 99.82 mL
0.5 mM 2 mL 9.98 mL 19.96 mL
1 mM 1 mL 4.99 mL 9.98 mL
5 mM 0.2 mL 1 mL 2 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Grynkiewicz et al (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J.Biol.Chem. 260 3440 PMID: 3838314

Wang et al (2008) Sildenafil inhibits human pulmonary artery smooth muscle cell proliferation by decreasing capacitative Ca2+ entry. J.Pharmacol.Sci. 108 71 PMID: 18818482


If you know of a relevant reference for FURA-2AM, please let us know.

Keywords: FURA-2AM, FURA-2AM supplier, Fluorescent, Ca2+, indicator, Probes, Calcium, Signaling, Signalling, Agents, General, Ion, Indicators, and, Indicator, Dyes, 2220, Tocris Bioscience

3 Citations for FURA-2AM

Citations are publications that use Tocris products. Selected citations for FURA-2AM include:

Cabrita et al (2019) Niclosamide repurposed for the treatment of inflammatory airway disease. JCI Insight 4 PMID: 31391337

Bayliss and Evans (2013) Characterisation of AmphiAmR11, an amphioxus (Branchiostoma floridae) D2-DA-like G protein-coupled receptor. PLoS One 8 e80833 PMID: 24265838

Luo et al (2015) KA attenuates the Na+-dependent Ca2+ overload in rabbit ventricular myocytes in vitro by inhibiting late Na+ and L-type Ca2+ currents. Mol Neurodegener 36 1327 PMID: 26456586


Do you know of a great paper that uses FURA-2AM from Tocris? Please let us know.

Reviews for FURA-2AM

Average Rating: 4.7 (Based on 3 Reviews.)

5 Star
67%
4 Star
33%
3 Star
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2 Star
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1 Star
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Detects store-operated Ca2+ entry after ER store depletion in RBL-2H3 cells..
By Niroj Shrestha on 11/02/2018
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: RBL-2H3

RBL-2H3 cells at a confluency of 50–60% were cultured on glass coverslip and loaded with 2 μM Fura-2AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. The Fura-2 loaded cells were excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm every 1.2 s. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.

review image

FURA-2AM as a tool to analyse calcium transient in cardiomyocytes.
By Christopher Trummer on 10/15/2018
Assay Type: In Vitro
Species: Other
Cell Line/Tissue: primary ventricular cardiomyocytes

Primary guinea pig ventricular cardiomyocytes were loaded with FURA-2AM (1 μM) and Pluronic F 127 (1%) for 30 min followed by washing with normal-Tyrode solution. Cells were stimulated at 1 Hz frequency and fluorescence intensities were measured.

review image

Detects cytosolic calcium influx changes well.
By Anonymous on 11/01/2017
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: spinal cord neurons

Used in live cell calcium imaging assay to study glutamate receptor signaling in neurons. Used at a concentration of 5 micromolar (along with pluronic). Images shows changes in fura-2 fluorescence pre (left) and post(right) glutamate stimulation.

review image

Literature in this Area

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