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Description: Red-orange fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)
Chemical Name: 7-[(4R)-5-[[[[4-[3,6-Bis(dimethylamino)xanthylium-9-yl]-3-carboxyphenyl]amino]thioxomethyl]amino]-4-hydroxy-L-leucine]phalloidin
Purity: ≥90% (HPLC)
Protocols (1)
Literature (1)

Biological Activity for Phalloidin-TRITC

Key information: Phalloidin-TRITC is a green-fluorescent cytoskeleton stain. It binds and labels specifically F-actin.

Used for: Phalloidin-TRITC can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.

Application: fluorescent microscopy, immunofluorescence (IHC, ICC).

Properties and Photophysical Data: Phalloidin-TRITC is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Tetramethylrhodamine isothiocyanate (TRITC) dye. Phalloidin binds F-actin with high selectivity allowing Phalloidin-TRITC to stain actin with high-contrast discrimination. Excitation and emission maxima (λ) are 540 nm and 565 nm, respectively.

We suggest making up a stock solution of this product by dissolving the vial contents in 1.5 mL methanol or DMSO. Please refer to the protocol for further information on using this product.

Scientific Data

Fluorescence Imaging Applications in HeLa Cells for Phalloidin-TRITC View Larger

Applications in HeLa Cells for Phalloidin-TRITC. Application of Phalloidin-TRITC: HeLa cells were labeled with 150 nM Phalloidin-TRITC for 20 minutes. Counterstained with DAPI (Catalog # 5748).

Technical Data for Phalloidin-TRITC

M. Wt 1231.4
Formula C60H70N12O13S2
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 915013-10-4
Smiles C[C@H](O)[C@H]1NC(=O)C(C)NC(=O)[C@H](C[C@@](C)(O)CNC(=S)NC2=CC(C([O-])=O)=C(C=C2)C2=C3C=CC(C=C3OC3=C2C=CC(=C3)N(C)C)=[N+](C)C)NC(=O)[C@@H]2CC3=C(NC4=C3C=CC=C4)SC[C@@H](NC1=O)C(=O)N1C[C@H](O)C[C@H]1C(=O)N[C@@H](C)C(=O)N2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Phalloidin-TRITC

Certificate of Analysis / Product Datasheet
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References for Phalloidin-TRITC

References are publications that support the biological activity of the product.

If you know of a relevant reference for Phalloidin-TRITC, please let us know.

Keywords: Phalloidin-TRITC, Phalloidin-TRITC supplier, red, orange, fluorescent, cytoskeleton, actin, filament, stain, probe, Organelle, and, Cell, Structure, Stains, Fluorescent, Actin, Probes, 5783, Tocris Bioscience

Citations for Phalloidin-TRITC

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Protocols for Phalloidin-TRITC

The following protocol features additional information for the use of Phalloidin-TRITC (Cat. No. 5783).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals