This is intended as a guide only - optimization may be needed.
Phalloidin is a phallotoxin isolated from Amanita phalloides, the death cap mushroom. Fluorescent phallotoxins stain F-actin at nanomolar concentrations. They are water soluble and produce low non-specific binding.
Dissolve the vial contents in 1.5 mL methanol or DMSO. This will yield a solution with a final concentration of approximately 7.3 μM.
This stock solution can be further diluted as required for the intended application, we recommend a dilution of approximately 40X. For more information on preparing stock solutions, dilutions and reconstitution please see our Molarity Calculator, Dilution Calculator and Reconstitution Calculator.
Stock solutions can be frozen for one year when stored at ≤–20°C, desiccated, and protected from light.
Prepare a 1 mL solution containing 50 to 100 μg/mL lysopalmitoylphosphatidylcholine and 3.7% formaldehyde. Add approximately 50 μL of Phalloidin-FITC methanolic stock solution Apply staining solution to cells and leave for 20 minutes at 4°C. 3X Wash cells with PBS. View coverslip.
Phallotoxins are usually not cell-permeable, but it is possible to label some types of cells. Pinocytosis, microinjections and unknown entry methods by hepatocytes have all been documented. Please conduct a literature search to find an appropriate protocol for your experiments.