PD 407824

Pricing Availability Delivery Time Qty
Cat.No. 2694 - PD 407824 | C20H12N2O3 | CAS No. 622864-54-4
Description: Selective inhibitor of Chk1 and Wee1
Chemical Name: 9-Hydroxy-4-phenyl-pyrrolo[3,4-c]carbazole-1,3(2H,6H)-dione
Purity: ≥98% (HPLC)
Datasheet
Citations (2)
Literature

Biological Activity

Selective inhibitor of checkpoint kinases Chk1 and Wee1 (IC50 values are 47 and 97 nM respectively). Displays selectivity over a range of other protein kinases; IC50 values are 3.4, 3.75, > 5, > 50, > 50 and > 50 μM for PKC, CDK4, other CDKs, c-Src, PDGFR and FGFR respectively.

Licensing Information

Sold for research purposes under agreement from Pfizer Inc.

Compound Libraries

PD 407824 is also offered as part of the Tocriscreen Plus and Tocriscreen Kinase Inhibitor Toolbox I. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 328.32
Formula C20H12N2O3
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 622864-54-4
PubChem ID 4369491
InChI Key IAUZTOZLTFSMIE-UHFFFAOYSA-N
Smiles OC1=CC2=C(NC3=C2C2=C(C(=O)NC2=O)C(=C3)C2=CC=CC=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 32.83 100
ethanol 8.2 25

Preparing Stock Solutions

The following data is based on the product molecular weight 328.32. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.05 mL 15.23 mL 30.46 mL
5 mM 0.61 mL 3.05 mL 6.09 mL
10 mM 0.3 mL 1.52 mL 3.05 mL
50 mM 0.06 mL 0.3 mL 0.61 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Squire et al (2005) Structure and inhibition of the human cell cycle checkpoint kinase, Wee1A kinase: an atypical tyrosine kinase with a key role in CDK1 regulation. Structure 13 541 PMID: 15837193

Palmer et al (2006) 4-Phenylpyrrolo[3,4-c]carbazole-1,3(2H,6H)-dione inhibitors of the checkpoint kinase Wee1. Structure-activity relationships for chromophore modification and phenylring substitutions. J.Med.Chem. 49 4896 PMID: 16884302


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View Related Products by Product Action

View all Checkpoint Kinase Inhibitors

Keywords: PD 407824, supplier, Selective, inhibitors, inhibits, Chk1, Wee1, Checkpoint, Control, Kinases, PD407824, Pfizer, Checkpoint, Kinases, Checkpoint, Control, Kinases, Other, Kinases, Checkpoint, Kinases, Tocris Bioscience

2 Citations for PD 407824

Citations are publications that use Tocris products. Selected citations for PD 407824 include:

Pouliot et al (2012) Cisplatin sensitivity mediated by WEE1 and CHK1 is mediated by miR-155 and the miR-15 family. Cancer Res 72 5945 PMID: 22942255

Kishikawa et al (2015) Decreased miR122 in hepatocellular carcinoma leads to chemoresistance with increased arginine. Oncotarget 6 8339 PMID: 25826076


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Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.