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OG 488 BAPTA-1 AM
Cell-permeable, fluorescently labeled Ca2+ indicator. Composed of a green fluorescent probe (OG 488, Cat. No. 6257) conjugated to a cell-permeable Ca2+ chelator (BAPTA AM, Cat. No. 2787). Fluorescent intensity increases approximately 14-fold from resting intensity, upon Ca2+ binding. Displays uniform cellular distribution with minimal subcellular compartmentalization. Excitation/emission λ 494/523 nm.
Optical Data for OG 488 BAPTA-1 AM
|Extinction Coefficient (ε)||19000 M-1cm-1|
|Quantum Yield (φ)||0.91|
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 1258.06. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.01 mM||79.49 mL||397.44 mL||794.87 mL|
|0.05 mM||15.9 mL||79.49 mL||158.97 mL|
|0.1 mM||7.95 mL||39.74 mL||79.49 mL|
|0.5 mM||1.59 mL||7.95 mL||15.9 mL|
References are publications that support the biological activity of the product.
Thomas et al (2000) A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals Cell Calcium 28 213 PMID: 11032777
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