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Description: Cell-permeable, fluorescent Ca2+ indicator
Chemical Name: N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[4-[[[3',6'-bis(acetyloxy)-2',7'-difluoro-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]amino]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester
Purity: ≥90% (HPLC)
Literature (1)

Biological Activity for OG 488 BAPTA-1 AM

OG 488 BAPTA-1 AM is a cell-permeable, fluorescently labeled Ca2+ indicator (Kd =170 nM). Composed of a green fluorescent probe (OG 488, Cat. No. 6257) conjugated to a cell-permeable Ca2+ chelator (BAPTA AM, Cat. No. 2787). Fluorescent intensity increases approximately 14-fold from resting intensity, upon Ca2+ binding. Displays uniform cellular distribution with minimal subcellular compartmentalization. Excitation/emission λ 494/523 nm.

Optical Data for OG 488 BAPTA-1 AM

Emission Color Green
λabs 494 nm
λem 523 nm
Extinction Coefficient (ε) 19000 M-1cm-1
Quantum Yield (φ) 0.91
Closest Laser line 488 nm
Cell Permeable Yes
Application Flow Cytometry, Fluorescence Microscopy

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Technical Data for OG 488 BAPTA-1 AM

M. Wt 1258.06
Formula C59H53F2N3O26
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 244167-57-5
PubChem ID 102028682
Smiles FC1=CC2=C(C3=CC=C(C(NC4=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC5=CC=CC=C5N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4)=O)C=C3C([O-])=O)C6=CC(F)=C(OC(C)=O)C=C6[O+]=C2C=C1OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for OG 488 BAPTA-1 AM

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 1.26 1

Preparing Stock Solutions for OG 488 BAPTA-1 AM

The following data is based on the product molecular weight 1258.06. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.01 mM 79.49 mL 397.44 mL 794.87 mL
0.05 mM 15.9 mL 79.49 mL 158.97 mL
0.1 mM 7.95 mL 39.74 mL 79.49 mL
0.5 mM 1.59 mL 7.95 mL 15.9 mL

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Product Datasheets for OG 488 BAPTA-1 AM

Certificate of Analysis / Product Datasheet
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References for OG 488 BAPTA-1 AM

References are publications that support the biological activity of the product.

Thomas et al (2000) A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals Cell Calcium 28 213 PMID: 11032777

If you know of a relevant reference for OG 488 BAPTA-1 AM, please let us know.

Keywords: OG 488 BAPTA-1 AM, OG 488 BAPTA-1 AM supplier, OG488, BAPTA-1AM, fluorescent, calcium, indicator, chelator, cell, permeable, Ion, Indicators, General, Calcium, Signaling, Agents, Fluorescent, 6256, Tocris Bioscience

Citations for OG 488 BAPTA-1 AM

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