OG 488, SE

Discontinued Product

OG 488, SE (Cat. No. 6257) has been withdrawn from sale for commercial reasons.
Description: Green fluorescent dye; supplied as NHS ester
Chemical Name: 2',7'-Difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid 2,5-dioxo-1-pyrrolidinyl ester
Protocols (1)
Literature (1)

Biological Activity for OG 488, SE

OG 488, SE is a green fluorescent dye. pH-insensitive in physiological pH range (pKa = 4.6). Can be used to monitor pH change in acidic organelles or pathways. Excitation/emission λ 501/526 nm.

Optical Data for OG 488, SE

OG 488, SE Dye Spectra
Emission Color Green
λabs 496 nm
λem 516 nm
Extinction Coefficient (ε) 76,000 M-1cm-1
Quantum Yield (φ) 0.9
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
pH Sensitivity pH-insensitive in physiological pH range (pKa = 4.6)
Correction Factor 280 0.12
Application Imaging, acidic organelle probe, flow, STED
Alternative For TFAX 488, SE, 5(6)-SFX (Fluorescein), SE, FITC,

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for OG 488, SE

M. Wt 509.37
Formula C25H13F2NO9
Storage Store at -20°C
CAS Number 198139-51-4
PubChem ID 58720805
Smiles OC1=C(F)C=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C(O)=O)=C(C=C4F)C(O5)=CC4=O)C5=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

References for OG 488, SE

References are publications that support the biological activity of the product.

Vergene et al (1998) Phagosomal pH determination by dual fluorescence flow cytometry. Anal.Biochem. 255 127 PMID: 9448851

Keywords: OG 488, SE, OG 488, SE supplier, OG488SE, green, fluorescent, dye, NHS, ester, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, Flow, Cytometry, 6257, Tocris Bioscience

Citations for OG 488, SE

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Currently there are no citations for OG 488, SE.

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Protocols for OG 488, SE

The following protocol features additional information for the use of OG 488, SE (Cat. No. 6257).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
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  • Tissue Clearing Kits and Reagents
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