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Submit ReviewOG 488, SE
Description: Green fluorescent dye; supplied as NHS ester
Chemical Name: 2',7'-Difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid 2,5-dioxo-1-pyrrolidinyl ester
Biological Activity
Green fluorescent dye. pH-insensitive in physiological pH range (pKa = 4.6). Can be used to monitor pH change in acidic organelles or pathways. Excitation/emission λ 501/526 nm.
Technical Data
| M. Wt | 509.37 |
| Formula | C25H13F2NO9 |
| Storage | Store at -20°C |
| CAS Number | 198139-51-4 |
| PubChem ID | 58720805 |
| InChI Key | RPOLNGNGJOEMSR-UHFFFAOYSA-N |
| Smiles | OC1=C(F)C=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C(O)=O)=C(C=C4F)C(O5)=CC4=O)C5=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 0.51 | 1 |
Preparing Stock Solutions
The following data is based on the product molecular weight 509.37. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.01 mM | 196.32 mL | 981.6 mL | 1963.21 mL |
| 0.05 mM | 39.26 mL | 196.32 mL | 392.64 mL |
| 0.1 mM | 19.63 mL | 98.16 mL | 196.32 mL |
| 0.5 mM | 3.93 mL | 19.63 mL | 39.26 mL |
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Product Datasheets
References
References are publications that support the biological activity of the product.
Vergene et al (1998) Phagosomal pH determination by dual fluorescence flow cytometry. Anal.Biochem. 255 127 PMID: 9448851
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Keywords: OG 488, SE, OG 488, SE supplier, OG488SE, green, fluorescent, dye, NHS, ester, Other, Fluorescent, Dyes, 6257, Tocris Bioscience
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