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OG 488, SE

Cat. No. 6257 Submit a Review Datasheet / COA / SDS
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Cat.No. 6257 - OG 488, SE | C25H13F2NO9 | CAS No. 198139-51-4
Description: Green fluorescent dye; supplied as NHS ester
Chemical Name: 2',7'-Difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid 2,5-dioxo-1-pyrrolidinyl ester
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Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Green fluorescent dye. pH-insensitive in physiological pH range (pKa = 4.6). Can be used to monitor pH change in acidic organelles or pathways. Excitation/emission λ 501/526 nm.

Technical Data

M. Wt 509.37
Formula C25H13F2NO9
Storage Store at -20°C
CAS Number 198139-51-4
PubChem ID 58720805
InChI Key RPOLNGNGJOEMSR-UHFFFAOYSA-N
Smiles OC1=C(F)C=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C(O)=O)=C(C=C4F)C(O5)=CC4=O)C5=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 0.51 1

Preparing Stock Solutions

The following data is based on the product molecular weight 509.37. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.01 mM 196.32 mL 981.6 mL 1963.21 mL
0.05 mM 39.26 mL 196.32 mL 392.64 mL
0.1 mM 19.63 mL 98.16 mL 196.32 mL
0.5 mM 3.93 mL 19.63 mL 39.26 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Vergene et al (1998) Phagosomal pH determination by dual fluorescence flow cytometry. Anal.Biochem. 255 127 PMID: 9448851

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