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OG 488, SE

Cat. No. 6257 Submit a Review Datasheet / COA / SDS
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Cat.No. 6257 - OG 488, SE | C25H13F2NO9 | CAS No. 198139-51-4
Description: Green fluorescent dye; supplied as NHS ester
Chemical Name: 2',7'-Difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid 2,5-dioxo-1-pyrrolidinyl ester
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Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Green fluorescent dye. pH-insensitive in physiological pH range (pKa = 4.6). Can be used to monitor pH change in acidic organelles or pathways. Excitation/emission λ 501/526 nm.

Optical Data for OG 488, SE

Emission Color Green
Brightness
λabs 496 nm
λem 516 nm
Extinction Coefficient (ε) 76,000 M-1cm-1
Quantum Yield (φ) 0.9
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
pH Sensitivity pH-insensitive in physiological pH range (pKa = 4.6)
Correction Factor 280 0.12
Application Imaging, acidic organelle probe, flow, STED
Alternative For TFAX 488, SE, 5(6)-SFX (Fluorescein), SE, FITC,

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M. Wt 509.37
Formula C25H13F2NO9
Storage Store at -20°C
CAS Number 198139-51-4
PubChem ID 58720805
InChI Key RPOLNGNGJOEMSR-UHFFFAOYSA-N
Smiles OC1=C(F)C=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C(O)=O)=C(C=C4F)C(O5)=CC4=O)C5=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 0.51 1

Preparing Stock Solutions

The following data is based on the product molecular weight 509.37. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.01 mM 196.32 mL 981.6 mL 1963.21 mL
0.05 mM 39.26 mL 196.32 mL 392.64 mL
0.1 mM 19.63 mL 98.16 mL 196.32 mL
0.5 mM 3.93 mL 19.63 mL 39.26 mL

Molarity Calculator

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Vergene et al (1998) Phagosomal pH determination by dual fluorescence flow cytometry. Anal.Biochem. 255 127 PMID: 9448851

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