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Submit ReviewOG 488, SE
Description: Green fluorescent dye; supplied as NHS ester
Chemical Name: 2',7'-Difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid 2,5-dioxo-1-pyrrolidinyl ester
Biological Activity
Green fluorescent dye. pH-insensitive in physiological pH range (pKa = 4.6). Can be used to monitor pH change in acidic organelles or pathways. Excitation/emission λ 501/526 nm.
Optical Data for OG 488, SE
| Emission Color | Green |
|---|---|
| Brightness | |
| λabs | 496 nm |
| λem | 516 nm |
| Extinction Coefficient (ε) | 76,000 M-1cm-1 |
| Quantum Yield (φ) | 0.9 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| pH Sensitivity | pH-insensitive in physiological pH range (pKa = 4.6) |
| Correction Factor 280 | 0.12 |
| Application | Imaging, acidic organelle probe, flow, STED |
| Alternative For | TFAX 488, SE, 5(6)-SFX (Fluorescein), SE, FITC, |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
| M. Wt | 509.37 |
| Formula | C25H13F2NO9 |
| Storage | Store at -20°C |
| CAS Number | 198139-51-4 |
| PubChem ID | 58720805 |
| InChI Key | RPOLNGNGJOEMSR-UHFFFAOYSA-N |
| Smiles | OC1=C(F)C=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C(O)=O)=C(C=C4F)C(O5)=CC4=O)C5=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 0.51 | 1 |
Preparing Stock Solutions
The following data is based on the product molecular weight 509.37. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.01 mM | 196.32 mL | 981.6 mL | 1963.21 mL |
| 0.05 mM | 39.26 mL | 196.32 mL | 392.64 mL |
| 0.1 mM | 19.63 mL | 98.16 mL | 196.32 mL |
| 0.5 mM | 3.93 mL | 19.63 mL | 39.26 mL |
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Product Datasheets
References
References are publications that support the biological activity of the product.
Vergene et al (1998) Phagosomal pH determination by dual fluorescence flow cytometry. Anal.Biochem. 255 127 PMID: 9448851
If you know of a relevant reference for OG 488, SE, please let us know.
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Keywords: OG 488, SE, OG 488, SE supplier, OG488SE, green, fluorescent, dye, NHS, ester, Other, Fluorescent, Dyes, 6257, Tocris Bioscience
Citations for OG 488, SE
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Protocols for OG 488, SE
The following protocol features additional information for the use of OG 488, SE (Cat. No. 6257).
Literature in this Area
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