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Description: Neurogenic agent; induces neuronal differentiation of SVZ progenitors and also induces cardiomyogenic differentiation
Chemical Name: N-Cyclopropyl-5-(2-thienyl)-3-isoxazolecarboxamide
Purity: ≥98% (HPLC)
Citations (3)
Protocols (1)
Literature (7)

Biological Activity for ISX 9

ISX 9 is a neurogenic agent. Mediates neuroD reporter gene induction via activation of Ca2+ influx; increases expression of neurogenic differentiation 1 (NeuroD1) transcription factor. Induces neuronal differentiation in human cortical neuronal cells (HCN), adult mouse whole brain (MWB) and subventricular zone (SVZ) progenitors. ISX 9 is also shown to induce cardiomyogenic differentiation of Notch-activated epicardium-derived cells in vitro. When used in combination with other small molecules, ISX 9 directs differentiation of pancreatic progenitors towards β cells.

ISX 9 synthesized to Ancillary Material Grade also available.

For more information about how ISX 9 may be used, see our protocol: Transdifferentiating Fibroblasts into Neurons.

Compound Libraries for ISX 9

ISX 9 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for ISX 9

M. Wt 234.27
Formula C11H10N2O2S
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 832115-62-5
PubChem ID 19582717
Smiles O=C(NC2CC2)C1=NOC(C3=CC=CS3)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for ISX 9

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 23.43 100
ethanol 11.71 50

Preparing Stock Solutions for ISX 9

The following data is based on the product molecular weight 234.27. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.27 mL 21.34 mL 42.69 mL
5 mM 0.85 mL 4.27 mL 8.54 mL
10 mM 0.43 mL 2.13 mL 4.27 mL
50 mM 0.09 mL 0.43 mL 0.85 mL

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Product Datasheets for ISX 9

Certificate of Analysis / Product Datasheet
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References for ISX 9

References are publications that support the biological activity of the product.

Schneider et al (2008) Small-molecule activation of neuronal cell fate. Nat.Chem.Biol 4 408 PMID: 18552832

Dioum et al (2011) A small molecule differentiation inducer increases Ins production by pancreatic β cells. Proc.Natl.Acad.Sci.U.S.A. 108 20713 PMID: 22143803

Zhang et al (2011) Small-molecule blocks malignant astrocyte proliferation and induces neuronal gene expression. Differentiation 81 233 PMID: 21419563

Russell et al (2012) Targeting native adult heart progenitors with cardiogenic small molecules. ACS Chem.Biol. 7 1067 PMID: 22413910

Liu et al (2021) Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells. Nat.Commun. 12 3330 PMID: 34099664

If you know of a relevant reference for ISX 9, please let us know.

Keywords: ISX 9, ISX 9 supplier, ISX9, isoxazoles, neurogenic, neuronal, differentiation, neuroD1, CaMKII, MEF2, neurogenesis, Isx1, NeuroD1, Neuronal, differentiator, inducer, III, beta, β, cells, cardiomyocyte, Neural, Stem, Cells, Cardiomyocyte, Organoids, 4439, Tocris Bioscience

3 Citations for ISX 9

Citations are publications that use Tocris products. Selected citations for ISX 9 include:

Koh et al (2015) Differential Effects of Isoxazole-9 on Neural Stem/Progenitor Cells, Oligodendrocyte Precursor Cells, and Endothelial Progenitor Cells. PLoS One 10 e0138724 PMID: 26407349

Xie et al (2018) β-actin regulates a heterochromatin landscape essential for optimal induction of neuronal programs during direct reprograming. PLoS Genet 14 e1007846 PMID: 30557298

Pujol et al (2018) Isx9 Regulates Calbindin D28K Expression in Pancreatic β Cells and Promotes β Cell Survival and Function. Int J Mol Sci 19 PMID: 30150605

Do you know of a great paper that uses ISX 9 from Tocris? Please let us know.

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Protocols for ISX 9

The following protocol features additional information for the use of ISX 9 (Cat. No. 4439).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

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