endo-IWR 1

Pricing Availability Delivery Time Qty
Cat.No. 3532 - endo-IWR 1 | C25H19N3O3 | CAS No. 1127442-82-3
Description: Wnt/β-catenin signaling inhibitor; axin stabilizer
Chemical Name: [(3aR*,4S*,7R*,7aS)-1,3,3a,4,7,7a-Hexahydro-1,3-dioxo-4,7-methano-2H-isoindol-2-yl]-N-8-quinolinylbenzamide
Purity: ≥98% (HPLC)
Datasheet
Citations (4)
Literature
Pathways

Biological Activity

Inhibitor of Wnt signaling. Induces an increase in Axin2 protein levels; promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes. Negative control exo-IWR (Cat. No. 3947) available.

Compound Libraries

endo-IWR 1 is also offered as part of the Tocriscreen Plus and Tocriscreen Stem Cell Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 409.44
Formula C25H19N3O3
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 1127442-82-3
PubChem ID 90488854
InChI Key MFEADGGBEDIZRE-MGIYQXMMSA-N
Smiles [H][C@]13[C@](C(N(C4=CC=C(C(NC5=CC=CC6=C5N=CC=C6)=O)C=C4)C3=O)=O)([H])[C@H]2C=C[C@@H]1C2.[H][C@@]79[C@@](C(N(C%10=CC=C(C(NC%11=CC=CC%12=C%11N=CC=C%12)=O)C=C%10)C9=O)=O)([H])[C@@H]8C=C[C@H]7C8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 40.94 100

Preparing Stock Solutions

The following data is based on the product molecular weight 409.44. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.44 mL 12.21 mL 24.42 mL
5 mM 0.49 mL 2.44 mL 4.88 mL
10 mM 0.24 mL 1.22 mL 2.44 mL
50 mM 0.05 mL 0.24 mL 0.49 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Lu et al (2009) Structure-activity relationship studies of small-molecule inhibitors of Wnt response. Bioorg.Med.Chem.Lett. 19 3825 PMID: 19410457

Chen et al (2008) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chem.Biol. 5 100 PMID:


If you know of a relevant reference for endo-IWR 1, please let us know.

Keywords: endo-IWR 1, supplier, endo-IWR1, wnt, signaling, signalling, inhibitors, inhibits, axin, axin2, stabilizer, stabiliser, IWR1, beta-catenin, β-catenin, b-catenin, Beta-catenin, Stem, Cell, Signaling, Beta-catenin, Tocris Bioscience

4 Citations for endo-IWR 1

Citations are publications that use Tocris products. Selected citations for endo-IWR 1 include:

Latos et al (2015) Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells. Nat Commun 6 7776 PMID: 26206133

Lindström et al (2015) Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron. Mol Cell Biol 3 e04000 PMID: 25647637

Suzuki et al (2015) WNT/β-Catenin Signaling Regulates Multiple Steps of Myogenesis by Regulating Step-Specific Targets. Development 35 1763 PMID: 25755281

Iwata et al (2014) TGFβ regulates epithelial-mesenchymal interactions through WNT signaling activity to control muscle development in the soft palate. Nat Protoc 141 909 PMID: 24496627


Do you know of a great paper that uses endo-IWR 1 from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Pathways for endo-IWR 1

Wnt

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.

Protocols

TODO: Add Protocols