endo-IWR 1

Pricing Availability   Qty
Cat.No. 3532 - endo-IWR 1 | C25H19N3O3 | CAS No. 1127442-82-3
Description: Wnt/β-catenin signaling inhibitor; axin stabilizer
Chemical Name: [(3aR*,4S*,7R*,7aS)-1,3,3a,4,7,7a-Hexahydro-1,3-dioxo-4,7-methano-2H-isoindol-2-yl]-N-8-quinolinylbenzamide
Purity: ≥98% (HPLC)
Datasheet
Citations (10)
Reviews
Protocols (1)
Literature
Pathways

Biological Activity

Inhibitor of Wnt signaling. Induces an increase in Axin2 protein levels; promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.

Negative Control also available.

Compound Libraries

endo-IWR 1 is also offered as part of the Tocriscreen Plus and Tocriscreen Stem Cell Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 409.44
Formula C25H19N3O3
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 1127442-82-3
PubChem ID 90488854
InChI Key MFEADGGBEDIZRE-MGIYQXMMSA-N
Smiles [H][C@]13[C@](C(N(C4=CC=C(C(NC5=CC=CC6=C5N=CC=C6)=O)C=C4)C3=O)=O)([H])[C@H]2C=C[C@@H]1C2.[H][C@@]79[C@@](C(N(C%10=CC=C(C(NC%11=CC=CC%12=C%11N=CC=C%12)=O)C=C%10)C9=O)=O)([H])[C@@H]8C=C[C@H]7C8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 40.94 100

Preparing Stock Solutions

The following data is based on the product molecular weight 409.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.44 mL 12.21 mL 24.42 mL
5 mM 0.49 mL 2.44 mL 4.88 mL
10 mM 0.24 mL 1.22 mL 2.44 mL
50 mM 0.05 mL 0.24 mL 0.49 mL

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References

References are publications that support the biological activity of the product.

Lu et al (2009) Structure-activity relationship studies of small-molecule inhibitors of Wnt response. Bioorg.Med.Chem.Lett. 19 3825 PMID: 19410457

Chen et al (2008) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chem.Biol. 5 100 PMID: 9125156

Kadoshima et al (2013) Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc.Natl.Acad.Sci.USA 110 20284 PMID: 24277810

Lancaster et al (2015) Generation of Cerebral Organoids from Human Pluripotent Stem Cells Nat. protoc. 9 2329 PMID: 25188634


If you know of a relevant reference for endo-IWR 1, please let us know.

Keywords: endo-IWR 1, endo-IWR 1 supplier, endo-IWR1, wnt, signaling, signalling, inhibitors, inhibits, axin, axin2, stabilizer, stabiliser, IWR1, beta-catenin, β-catenin, b-catenin, Beta-catenin, Stem, Cell, Signaling, Organoids, 3532, Tocris Bioscience

10 Citations for endo-IWR 1

Citations are publications that use Tocris products. Selected citations for endo-IWR 1 include:

Yamoah et al (2018) Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles. Int J Nanomedicine 13 6073 PMID: 30323594

Suzuki et al (2018) The role of acetyltransferases for the temporal-specific accessibility of β-catenin to the myogenic gene locus. Sci Rep 8 15057 PMID: 30305648

Gao et al (2019) Establishment of porcine and human expanded potential stem cells. Nat Cell Bio 21 687 PMID: 31160711

Lindström et al (2015) Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron. Mol Cell Biol 3 e04000 PMID: 25647637

Suzuki et al (2015) WNT/β-Catenin Signaling Regulates Multiple Steps of Myogenesis by Regulating Step-Specific Targets. Development 35 1763 PMID: 25755281

Cajal et al (2014) A conserved role for non-neural ectoderm cells in early neural development. Development 141 4127 PMID: 25273086

Kennedy et al (2016) Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription. Proc Natl Acad Sci U S A 113 3545 PMID: 26969725

Kishimoto (2017) Wnt/β-catenin signaling suppresses expressions of Scx, Mkx, and Tnmd in tendon-derived cells. PLoS One 12 e0182051 PMID: 28750046

Latos et al (2015) Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells. Nat Commun 6 7776 PMID: 26206133

Iwata et al (2014) TGFβ regulates epithelial-mesenchymal interactions through WNT signaling activity to control muscle development in the soft palate. Nat Protoc 141 909 PMID: 24496627


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Protocols for endo-IWR 1

The following protocol features additional information for the use of endo-IWR 1 (Cat. No. 3532).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Pathways for endo-IWR 1

Wnt

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.