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endo-IWR 1 is an inhibitor of Wnt signaling. Induces an increase in axin2 protein levels; promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes. Promotes endothelial cell specification of cardiac progenitor cells and may be used in protocols for the generation of retinal pigment epithelial cells from hPSCs.
Negative Control also available.
For more information about how endo-IWR 1 may be used, see our protocol: Generation and Propagation of EPS cells (LCDM Cocktail).
endo-IWR 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 409.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.44 mL||12.21 mL||24.42 mL|
|5 mM||0.49 mL||2.44 mL||4.88 mL|
|10 mM||0.24 mL||1.22 mL||2.44 mL|
|50 mM||0.05 mL||0.24 mL||0.49 mL|
References are publications that support the biological activity of the product.
Lu et al (2009) Structure-activity relationship studies of small-molecule inhibitors of Wnt response. Bioorg.Med.Chem.Lett. 19 3825 PMID: 19410457
Chen et al (2008) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chem.Biol. 5 100 PMID: 19125156
Kadoshima et al (2013) Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc.Natl.Acad.Sci.USA 110 20284 PMID: 24277810
Lancaster et al (2015) Generation of cerebral organoids from human pluripotent stem cells. Nat.Protoc. 9 2329 PMID: 25188634
Reichman et al (2018) Wnt inhibition promotes vascular specification of embryonic cardiac progenitors. Development 145 dev159905 PMID: 29217753
Surendran et al (2022) An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells. STAR Protoc. 3 101803 PMID: 36386870
If you know of a relevant reference for endo-IWR 1, please let us know.
Keywords: endo-IWR 1, endo-IWR 1 supplier, endo-IWR1, wnt, signaling, signalling, inhibitors, inhibits, axin, axin2, stabilizer, stabiliser, IWR1, beta-catenin, β-catenin, b-catenin, cardiac, differentiation, RPE, retinal, pigment, epithelial, Beta-catenin, Stem, Cell, Signaling, Organoids, Retinal, Cells, 3532, Tocris Bioscience
Citations are publications that use Tocris products. Selected citations for endo-IWR 1 include:
Capeling et al (2022) Suspension culture promotes serosal mesothelial development in human intestinal organoids. Cell Rep. 38 110379 PMID: 35172130
Gao et al (2019) Establishment of porcine and human expanded potential stem cells. Nat Cell Bio 21 687 PMID: 31160711
Kishimoto (2017) Wnt/β-catenin signaling suppresses expressions of Scx, Mkx, and Tnmd in tendon-derived cells. PLoS One 12 e0182051 PMID: 28750046
Yamoah et al (2018) Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles. Int J Nanomedicine 13 6073 PMID: 30323594
Suzuki et al (2018) The role of acetyltransferases for the temporal-specific accessibility of β-catenin to the myogenic gene locus. Sci Rep 8 15057 PMID: 30305648
Lindström et al (2015) Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron. Mol Cell Biol 3 e04000 PMID: 25647637
Suzuki et al (2015) WNT/β-Catenin Signaling Regulates Multiple Steps of Myogenesis by Regulating Step-Specific Targets. Development 35 1763 PMID: 25755281
Cajal et al (2014) A conserved role for non-neural ectoderm cells in early neural development. Development 141 4127 PMID: 25273086
Garcia et al (2019) Huntington's Disease Patient-Derived Astrocytes Display Electrophysiological Impairments and Reduced Neuronal Support. Front Neurosci 13 669 PMID: 31316341
Kennedy et al (2016) Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription. Proc Natl Acad Sci U S A 113 3545 PMID: 26969725
Latos et al (2015) Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells. Nat Commun 6 7776 PMID: 26206133
Iwata et al (2014) TGFβ regulates epithelial-mesenchymal interactions through WNT signaling activity to control muscle development in the soft palate. Nat Protoc 141 909 PMID: 24496627
Do you know of a great paper that uses endo-IWR 1 from Tocris? Please let us know.
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The following protocol features additional information for the use of endo-IWR 1 (Cat. No. 3532).
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.