endo-IWR 1

Pricing Availability   Qty
Description: Wnt/β-catenin signaling inhibitor; axin stabilizer
Chemical Name: rel-4-[(3aR,4S,7R,7aS)-1,3,3a,4,7,7a-Hexahydro-1,3-dioxo-4,7-methano-2H-isoindol-2-yl]-N-8-quinolinylbenzamide
Purity: ≥98% (HPLC)
Datasheet
Citations (26)
Reviews
Protocols (1)
Pathways (1)

Biological Activity for endo-IWR 1

endo-IWR 1 is an inhibitor of Wnt signaling. Induces an increase in axin2 protein levels; promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes. Promotes endothelial cell specification of cardiac progenitor cells and may be used in protocols for the generation of retinal pigment epithelial cells from hPSCs.

Negative Control also available.

endo-IWR 1 synthesized to Ancillary Material Grade also available.

For more information about how endo-IWR 1 may be used, see our protocol: Generation and Propagation of EPS cells (LCDM Cocktail).

Compound Libraries for endo-IWR 1

endo-IWR 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for endo-IWR 1

M. Wt 409.44
Formula C25H19N3O3
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 1127442-82-3
PubChem ID 90488854
InChI Key ZGSXEXBYLJIOGF-ALFLXDJESA-N
Smiles [H][C@]13[C@](C(N(C4=CC=C(C(NC5=CC=CC6=C5N=CC=C6)=O)C=C4)C3=O)=O)([H])[C@H]2C=C[C@@H]1C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for endo-IWR 1

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 40.94 100

Preparing Stock Solutions for endo-IWR 1

The following data is based on the product molecular weight 409.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.44 mL 12.21 mL 24.42 mL
5 mM 0.49 mL 2.44 mL 4.88 mL
10 mM 0.24 mL 1.22 mL 2.44 mL
50 mM 0.05 mL 0.24 mL 0.49 mL

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Product Datasheets for endo-IWR 1

Certificate of Analysis / Product Datasheet
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References for endo-IWR 1

References are publications that support the biological activity of the product.

Lu et al (2009) Structure-activity relationship studies of small-molecule inhibitors of Wnt response. Bioorg.Med.Chem.Lett. 19 3825 PMID: 19410457

Chen et al (2008) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chem.Biol. 5 100 PMID: 19125156

Kadoshima et al (2013) Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc.Natl.Acad.Sci.USA 110 20284 PMID: 24277810

Lancaster et al (2015) Generation of cerebral organoids from human pluripotent stem cells. Nat.Protoc. 9 2329 PMID: 25188634

Reichman et al (2018) Wnt inhibition promotes vascular specification of embryonic cardiac progenitors. Development 145 dev159905 PMID: 29217753

Surendran et al (2022) An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells. STAR Protoc. 3 101803 PMID: 36386870


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View all β-catenin Inhibitors

Keywords: endo-IWR 1, endo-IWR 1 supplier, endo-IWR1, wnt, signaling, signalling, inhibitors, inhibits, axin, axin2, stabilizer, stabiliser, IWR1, beta-catenin, β-catenin, b-catenin, cardiac, differentiation, RPE, retinal, pigment, epithelial, Beta-catenin, Stem, Cell, Signaling, Organoids, Retinal, Cells, 3532, Tocris Bioscience

26 Citations for endo-IWR 1

Citations are publications that use Tocris products. Selected citations for endo-IWR 1 include:

Latos et al (2015) Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells. Nat Commun 6 7776 PMID: 26206133

Iwata et al (2014) TGFβ regulates epithelial-mesenchymal interactions through WNT signaling activity to control muscle development in the soft palate. Nat Protoc 141 909 PMID: 24496627

Erika et al (2023) Induction of primordial germ cell-like cells from common marmoset embryonic stem cells by inhibition of WNT and retinoic acid signaling. Sci Rep 13 3186 PMID: 36823310

Yamoah et al (2018) Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles. Int J Nanomedicine 13 6073 PMID: 30323594

Suzuki et al (2018) The role of acetyltransferases for the temporal-specific accessibility of β-catenin to the myogenic gene locus. Sci Rep 8 15057 PMID: 30305648

Kennedy et al (2016) Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription. Proc Natl Acad Sci U S A 113 3545 PMID: 26969725

Garcia et al (2019) Huntington's Disease Patient-Derived Astrocytes Display Electrophysiological Impairments and Reduced Neuronal Support. Front Neurosci 13 669 PMID: 31316341

Esra et al (2021) Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Methods Mol Biol 2520 161-170 PMID: 34845657

Cajal et al (2014) A conserved role for non-neural ectoderm cells in early neural development. Development 141 4127 PMID: 25273086

Timothy C et al (2022) Contribution of Trp63CreERT2-labeled cells to alveolar regeneration is independent of tuft cells. Elife 11 PMID: 36129169

Martin et al (2022) A specialized bone marrow microenvironment for fetal haematopoiesis. Nat Commun 13 1327 PMID: 35288551

Alexandre P et al (2022) An epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates. Elife 11 PMID: 35536602

Min et al (2022) Comparison of chromatin accessibility landscapes during early development of prefrontal cortex between rhesus macaque and human. Nat Commun 13 3883 PMID: 35794099

W Hayes et al (2022) DDR1 contributes to kidney inflammation and fibrosis by promoting the phosphorylation of BCR and STAT3. JCI Insight 7 PMID: 34941574

Jia et al (2022) Selective activation and down-regulation of Trk receptors by neurotrophins in human neurons co-expressing TrkB and TrkC. J Neurochem 161 463-477 PMID: 35536742

Gao et al (2019) Establishment of porcine and human expanded potential stem cells. Nat Cell Bio 21 687 PMID: 31160711

Lindström et al (2015) Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron. Mol Cell Biol 3 e04000 PMID: 25647637

Suzuki et al (2015) WNT/β-Catenin Signaling Regulates Multiple Steps of Myogenesis by Regulating Step-Specific Targets. Development 35 1763 PMID: 25755281

Amos et al (2020) SEM/FIB Imaging for Studying Neural Interfaces. Dev Neurobiol 80 305-315 PMID: 31228876

Eunjeong et al (2020) TAZ/Wnt-β-catenin/c-MYC axis regulates cystogenesis in polycystic kidney disease. Proc Natl Acad Sci U S A 117 29001-29012 PMID: 33122431

Alon et al (2020) Electrophysiologic Characterization of Developing Human Embryonic Stem Cell-Derived Photoreceptor Precursors. Invest Ophthalmol Vis Sci 61 44 PMID: 32991686

Geoffrey et al (2020) Tissue-Specific Regulation of the Wnt/β-Catenin Pathway by PAGE4 Inhibition of Tankyrase. Cell Rep 32 107922 PMID: 32698014

Le et al (2020) Vascularized human cortical organoids (vOrganoids) model cortical development in vivo. PLoS Biol 18 e3000705 PMID: 32401820

Capeling et al (2022) Suspension culture promotes serosal mesothelial development in human intestinal organoids. Cell Rep. 38 110379 PMID: 35172130

Kishimoto (2017) Wnt/β-catenin signaling suppresses expressions of Scx, Mkx, and Tnmd in tendon-derived cells. PLoS One 12 e0182051 PMID: 28750046

Ali H et al (2018) hPSC Modeling Reveals that Fate Selection of Cortical Deep Projection Neurons Occurs in the Subplate. Cell Stem Cell 23 60-73.e6 PMID: 29937203


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Protocols for endo-IWR 1

The following protocol features additional information for the use of endo-IWR 1 (Cat. No. 3532).

Pathways for endo-IWR 1

Wnt Signaling Pathway

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.