endo-IWR 1

Cat. No. 3532 12 Citations Submit a Review Datasheet / COA / SDS

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Description: Wnt/β-catenin signaling inhibitor; axin stabilizer

Chemical Name: rel-4-[(3aR,4S,7R,7aS)-1,3,3a,4,7,7a-Hexahydro-1,3-dioxo-4,7-methano-2H-isoindol-2-yl]-N-8-quinolinylbenzamide

Purity: ≥98% (HPLC)

Datasheet

Citations (12)

Reviews

Protocols (1)

Literature (1)

Pathways (1)

Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for endo-IWR 1

endo-IWR 1 is an inhibitor of Wnt signaling. Induces an increase in axin2 protein levels; promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes. Promotes endothelial cell specification of cardiac progenitor cells and may be used in protocols for the generation of retinal pigment epithelial cells from hPSCs.

Negative Control also available.

For more information about how endo-IWR 1 may be used, see our protocol: Generation and Propagation of EPS cells (LCDM Cocktail).

Compound Libraries for endo-IWR 1

endo-IWR 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for endo-IWR 1

M. Wt	409.44
Formula	C₂₅H₁₉N₃O₃
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	1127442-82-3
PubChem ID	90488854
InChI Key	ZGSXEXBYLJIOGF-ALFLXDJESA-N
Smiles	[H][C@]13[C@](C(N(C4=CC=C(C(NC5=CC=CC6=C5N=CC=C6)=O)C=C4)C3=O)=O)([H])[C@H]2C=C[C@@H]1C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for endo-IWR 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	40.94	100

Preparing Stock Solutions for endo-IWR 1

The following data is based on the product molecular weight 409.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.44 mL	12.21 mL	24.42 mL
5 mM	0.49 mL	2.44 mL	4.88 mL
10 mM	0.24 mL	1.22 mL	2.44 mL
50 mM	0.05 mL	0.24 mL	0.49 mL

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Product Datasheets for endo-IWR 1

Certificate of Analysis / Product Datasheet

Safety Datasheet

References for endo-IWR 1

References are publications that support the biological activity of the product.

Lu et al (2009) Structure-activity relationship studies of small-molecule inhibitors of Wnt response. Bioorg.Med.Chem.Lett. 19 3825 PMID: 19410457

Chen et al (2008) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chem.Biol. 5 100 PMID: 19125156

Kadoshima et al (2013) Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc.Natl.Acad.Sci.USA 110 20284 PMID: 24277810

Lancaster et al (2015) Generation of cerebral organoids from human pluripotent stem cells. Nat.Protoc. 9 2329 PMID: 25188634

Reichman et al (2018) Wnt inhibition promotes vascular specification of embryonic cardiac progenitors. Development 145 dev159905 PMID: 29217753

Surendran et al (2022) An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells. STAR Protoc. 3 101803 PMID: 36386870

If you know of a relevant reference for endo-IWR 1, please let us know.

View Related Products by Target

Keywords: endo-IWR 1, endo-IWR 1 supplier, endo-IWR1, wnt, signaling, signalling, inhibitors, inhibits, axin, axin2, stabilizer, stabiliser, IWR1, beta-catenin, β-catenin, b-catenin, cardiac, differentiation, RPE, retinal, pigment, epithelial, Beta-catenin, Stem, Cell, Signaling, Organoids, Retinal, Cells, 3532, Tocris Bioscience

12 Citations for endo-IWR 1

Citations are publications that use Tocris products. Selected citations for endo-IWR 1 include:

Capeling et al (2022) Suspension culture promotes serosal mesothelial development in human intestinal organoids. Cell Rep. 38 110379 PMID: 35172130

Gao et al (2019) Establishment of porcine and human expanded potential stem cells. Nat Cell Bio 21 687 PMID: 31160711

Kishimoto (2017) Wnt/β-catenin signaling suppresses expressions of Scx, Mkx, and Tnmd in tendon-derived cells. PLoS One 12 e0182051 PMID: 28750046

Yamoah et al (2018) Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles. Int J Nanomedicine 13 6073 PMID: 30323594

Suzuki et al (2018) The role of acetyltransferases for the temporal-specific accessibility of β-catenin to the myogenic gene locus. Sci Rep 8 15057 PMID: 30305648

Lindström et al (2015) Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron. Mol Cell Biol 3 e04000 PMID: 25647637

Suzuki et al (2015) WNT/β-Catenin Signaling Regulates Multiple Steps of Myogenesis by Regulating Step-Specific Targets. Development 35 1763 PMID: 25755281

Cajal et al (2014) A conserved role for non-neural ectoderm cells in early neural development. Development 141 4127 PMID: 25273086

Garcia et al (2019) Huntington's Disease Patient-Derived Astrocytes Display Electrophysiological Impairments and Reduced Neuronal Support. Front Neurosci 13 669 PMID: 31316341

Kennedy et al (2016) Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription. Proc Natl Acad Sci U S A 113 3545 PMID: 26969725

Latos et al (2015) Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells. Nat Commun 6 7776 PMID: 26206133

Iwata et al (2014) TGFβ regulates epithelial-mesenchymal interactions through WNT signaling activity to control muscle development in the soft palate. Nat Protoc 141 909 PMID: 24496627

Do you know of a great paper that uses endo-IWR 1 from Tocris? Please let us know.

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Protocols for endo-IWR 1

The following protocol features additional information for the use of endo-IWR 1 (Cat. No. 3532).

Generation and Propagation of EPS cells (LCDM Cocktail)

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

Cancer Metabolism
Epigenetics in Cancer
Receptor Signaling
Cell Cycle and DNA Damage Repair
Angiogenesis
Invasion and Metastasis

Pathways for endo-IWR 1

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.