endo-IWR 1

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Description: Wnt/β-catenin signaling inhibitor; axin stabilizer
Chemical Name: rel-4-[(3aR,4S,7R,7aS)-1,3,3a,4,7,7a-Hexahydro-1,3-dioxo-4,7-methano-2H-isoindol-2-yl]-N-8-quinolinylbenzamide
Purity: ≥98% (HPLC)
Citations (12)
Protocols (1)
Literature (1)
Pathways (1)

Biological Activity for endo-IWR 1

endo-IWR 1 is an inhibitor of Wnt signaling. Induces an increase in axin2 protein levels; promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes. Promotes endothelial cell specification of cardiac progenitor cells

Negative Control also available.

For more information about how endo-IWR 1 may be used, see our protocol: Generation and Propagation of EPS cells (LCDM Cocktail).

Compound Libraries for endo-IWR 1

endo-IWR 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for endo-IWR 1

M. Wt 409.44
Formula C25H19N3O3
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 1127442-82-3
PubChem ID 90488854
Smiles [H][C@]13[C@](C(N(C4=CC=C(C(NC5=CC=CC6=C5N=CC=C6)=O)C=C4)C3=O)=O)([H])[C@H]2C=C[C@@H]1C2.[H][C@@]79[C@@](C(N(C%10=CC=C(C(NC%11=CC=CC%12=C%11N=CC=C%12)=O)C=C%10)C9=O)=O)([H])[C@@H]8C=C[C@H]7C8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for endo-IWR 1

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 40.94 100

Preparing Stock Solutions for endo-IWR 1

The following data is based on the product molecular weight 409.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.44 mL 12.21 mL 24.42 mL
5 mM 0.49 mL 2.44 mL 4.88 mL
10 mM 0.24 mL 1.22 mL 2.44 mL
50 mM 0.05 mL 0.24 mL 0.49 mL

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Product Datasheets for endo-IWR 1

Certificate of Analysis / Product Datasheet
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References for endo-IWR 1

References are publications that support the biological activity of the product.

Lu et al (2009) Structure-activity relationship studies of small-molecule inhibitors of Wnt response. Bioorg.Med.Chem.Lett. 19 3825 PMID: 19410457

Chen et al (2008) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chem.Biol. 5 100 PMID: 19125156

Kadoshima et al (2013) Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc.Natl.Acad.Sci.USA 110 20284 PMID: 24277810

Lancaster et al (2015) Generation of cerebral organoids from human pluripotent stem cells. Nat.Protoc. 9 2329 PMID: 25188634

Reichman et al (2018) Wnt inhibition promotes vascular specification of embryonic cardiac progenitors. Development 145 dev159905 PMID: 29217753

If you know of a relevant reference for endo-IWR 1, please let us know.

Keywords: endo-IWR 1, endo-IWR 1 supplier, endo-IWR1, wnt, signaling, signalling, inhibitors, inhibits, axin, axin2, stabilizer, stabiliser, IWR1, beta-catenin, β-catenin, b-catenin, cardiac, differentiation, Beta-catenin, Stem, Cell, Signaling, Organoids, 3532, Tocris Bioscience

12 Citations for endo-IWR 1

Citations are publications that use Tocris products. Selected citations for endo-IWR 1 include:

Capeling et al (2022) Suspension culture promotes serosal mesothelial development in human intestinal organoids. Cell Rep. 38 110379 PMID: 35172130

Gao et al (2019) Establishment of porcine and human expanded potential stem cells. Nat Cell Bio 21 687 PMID: 31160711

Kennedy et al (2016) Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription. Proc Natl Acad Sci U S A 113 3545 PMID: 26969725

Garcia et al (2019) Huntington's Disease Patient-Derived Astrocytes Display Electrophysiological Impairments and Reduced Neuronal Support. Front Neurosci 13 669 PMID: 31316341

Do you know of a great paper that uses endo-IWR 1 from Tocris? Please let us know.

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Protocols for endo-IWR 1

The following protocol features additional information for the use of endo-IWR 1 (Cat. No. 3532).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cancer Research Product Guide

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Pathways for endo-IWR 1

Wnt Signaling Pathway

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.