Doxorubicin hydrochloride

Pricing Availability   Qty
Cat.No. 2252 - Doxorubicin hydrochloride | C27H29NO11.HCl | CAS No. 25316-40-9
Description: Antitumor antibiotic agent. Inhibits DNA topoisomerase II
Alternative Names: Adriamycin, NSC 123127
Chemical Name: 10-[(3-Amino-2,3,6-trideoxy-α-L-lyxohexopyranosyl)oxy]-7,8,9,10-tetrahydro-6,8,11-trihydroxy-8-(hydroxyacetyl)-5,12-naphthacenedione hydrochloride
Purity: ≥98% (HPLC)
Citations (28)
Reviews (1)
Literature (2)

Biological Activity for Doxorubicin hydrochloride

Doxorubicin hydrochloride is an antitumor antibiotic agent that inhibits DNA topoisomerase II. DNA intercalator that inhibits nucleic acid synthesis and induces apoptosis. Reduces intracellular tau levels.

Compound Libraries for Doxorubicin hydrochloride

Doxorubicin hydrochloride is also offered as part of the Tocriscreen Antiviral Library and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Doxorubicin hydrochloride

M. Wt 579.99
Formula C27H29NO11.HCl
Storage Desiccate at RT
Purity ≥98% (HPLC)
CAS Number 25316-40-9
PubChem ID 443939
Smiles OC1=C(C[C@]([C@](CO)=O)(O)C[C@@H]4O[C@@]5([H])C[C@H](N)[C@H](O)[C@H](C)O5)C4=C(O)C3=C1C(C2=CC=CC(OC)=C2C3=O)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Doxorubicin hydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
water 29 50
DMSO 29 50

Preparing Stock Solutions for Doxorubicin hydrochloride

The following data is based on the product molecular weight 579.99. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 3.45 mL 17.24 mL 34.48 mL
2.5 mM 0.69 mL 3.45 mL 6.9 mL
5 mM 0.34 mL 1.72 mL 3.45 mL
25 mM 0.07 mL 0.34 mL 0.69 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Doxorubicin hydrochloride

Certificate of Analysis / Product Datasheet
Select another batch:

References for Doxorubicin hydrochloride

References are publications that support the biological activity of the product.

Skladanowski and Konopa (1993) Adriamycin and daunomycin induce programmed cell death (apoptosis) in tumour cells. Biochem.Pharmacol. 46 357 PMID: 8347161

Patel et al (1997) Identification of yeast DNA topoisomerase II mutants resistant to the antitumor drug doxorubicin: implications for the mechanisms of dox. action and cyotoxicity. Mol.Pharmacol. 52 658 PMID: 9380029

Gewirtz (1999) A critical evaluation of the mechanisms of action proposed for the antitumor effects of the anthracycline antibiotics adriamycin and daunoru. Biochem.Pharmacol. 57 727 PMID: 10075079

Dickey et al (2006) Pharmacologic reductions of total tau levels; implications for the role of microtubule dynamics in regulating tau expression. Mol.Neurodegen. 1 6 PMID: 16930453

If you know of a relevant reference for Doxorubicin hydrochloride, please let us know.

View Related Products by Product Action

View all DNA Topoisomerase Inhibitors

Keywords: Doxorubicin hydrochloride, Doxorubicin hydrochloride supplier, Antitumor, antibiotics, agent, inhibitors, inhibits, DNA, topoisomerase, II, Isomerases, NSC123127, chemotherapeutics, Adriamycin, NSC, 123127, Topoisomerase, Apoptosis, Inducers, Antibiotics, 2252, Tocris Bioscience

⚠ WARNING: This product can expose you to chemicals including Doxorubicin hydrochloride (Adriamycin), which is known to the State of California to cause cancer. For more information, go to

28 Citations for Doxorubicin hydrochloride

Citations are publications that use Tocris products. Selected citations for Doxorubicin hydrochloride include:

Aho et al (2016) Extracellular ATP protects endothelial cells against DNA damage. Purinergic Signal 12 575 PMID: 27030122

Thomas et al (2016) A Myocardial Slice Culture Model Reveals Alpha-1A-Adrenergic Receptor Signaling in the Human Heart. JACC Basic Transl Sci 1 155 PMID: 27453955

Gligorich et al (2013) Development of a screen to identify selective small molecules active against patient-derived metastatic and chemoresistant breast cancer cells. Breast Cancer Res 15 R58 PMID: 23879992

Natrajan et al (2012) Functional characterization of the 19q12 amplicon in grade III breast cancers. Breast Cancer Res 14 R53 PMID: 22433433

Iriondo (2018) TAK1 mediates microenvironment-triggered autocrine signals and promotes triple-negativebreast cancer lung metastasis. Nat Commun 9 1994 PMID: 29777109

Yuan et al (2017) Tenovin-6 impairs autophagy by inhibiting autophagic flux. Cell Death Dis 8 e2608 PMID: 28182004

Ayoub (2017) Crizotinib, a MET inhibitor, inhibits growth, migration, and invasion of breast cancer cells in vitro and synergizes with chemotherapeutic agents. Onco Targets Ther 10 4869 PMID: 29042798

Gharanei et al (2013) Attenuation of doxorubicin-induced cardiotoxicity by mdivi-1: a mitochondrial division/mitophagy inhibitor. PLoS One 8 e77713 PMID: 24147064

Lee et al (2019) Extracorporeal shock waves protect cardiomyocytes from DOX-induced cardiomyopathy by upregulating survivin via the integrin-ILK-Akt-Sp1/p53 axis. Sci Rep 9 12149 PMID: 31434946

Montgomery (2017) An Alpha-1A Adrenergic Receptor Agonist Prevents Acute dox. Cardiomyopathy in Male Mice. Plos One 12 e0168409 PMID: 28081170

El Zawily (2017) The EphB6 receptor is overexpressed in pediatric T cell acute lymphoblastic leukemia and increases its sensitivity to dox. treatment. Sci Rep 7 14767 PMID: 29116180

Toldo et al (2013) Comparative cardiac toxicity of anthracyclines in vitro and in vivo in the mouse. PLoS One 8 e58421 PMID: 23516478

Robinson et al (2013) RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs. PLoS One 8 e78641 PMID: 24265703

Guillon et al (2019) Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment. Cell Death Dis 10 199 PMID: 30814491

Yoon et al (2019) p53 induces senescence through Lamin A/C stabilization-mediated nuclear deformation. Cell Death Dis 10 107 PMID: 30728349

Zhitomirsky and Assaraf (2015) Lysosomal sequestration of hydrophobic weak base chemotherapeutics triggers lysosomal biogenesis and lysosome-dependent cancer multidrug resistance. Clin Transl Med 6 1143 PMID: 25544758

Kanchanapally et al (2019) Drug-loaded exosomal preparations from different cell types exhibit distinctive loading capability, yield, and antitumor efficacies: a comparative analysis. Int J Nanomedicine 14 531 PMID: 30666112

Ali et al (2017) Isolation and characterization of a new naturally immortalized human breast carcinoma cell line, KAIMRC1. BMC Cancer 17 803 PMID: 29187162

Beak et al (2017) An Oral Selective Alpha-1A Adrenergic Receptor Agonist Prevents Drug-induced Cardiotoxicity. JACC Basic Transl Sci 2 39 PMID: 28286875

Gallagher et al (2017) Lysosomotropism depends on glucose: a chloroquine resistance mechanism. Cell Death Dis 8 e3014 PMID: 28837152

Tavangar et al (2017) AMB potentiates the anticancer activity of Drug-induced on the MCF-7 breast cancer cells. J Chem Biol 10 143 PMID: 28685000

Smyth et al (2015) Biodistribution and delivery efficiency of unmodified tumor-derived exosomes. J Control Release 199 145 PMID: 25523519

Lim et al (2008) B cell translocation gene 2 enhances susceptibility of HeLa cells to doxorubicin-induced oxidative damage. J Biol Chem 283 33110 PMID: 18840609

Duff et al (2018) Regulation of senescence escape by the cdk4-EZH2-AP2M1 pathway in response to chemotherapy. Cell Death Dis 9 199 PMID: 29415991

Bussey et al (2016) Targeting polo-like kinase 1, a regulator of p53, in the treatment of adrenocortical carcinoma. PLoS One 5 1 PMID: 26754547

Thomas et al (2016) The α-1A Adrenergic Receptor in the Rabbit Heart. Mol Syst Biol 11 e0155238 PMID: 27258143

Sundaresan et al (2014) Dual-responsive polymer-coated iron oxide nanoparticles for drug delivery and imaging applications. Int J Pharm 466 1 PMID: 24607216

Hayano et al (2016) Loss of cysteinyl-tRNA synthetase (CARS) induces the transsulfuration pathway and inhibits ferroptosis induced by cystine deprivation. Cell Death Differ 23 270 PMID: 26184909

Do you know of a great paper that uses Doxorubicin hydrochloride from Tocris? Please let us know.

Reviews for Doxorubicin hydrochloride

Average Rating: (Based on 1 Review.)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Doxorubicin hydrochloride?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.