DMH-1

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Cat.No. 4126 - DMH-1 | C24H20N4O | CAS No. 1206711-16-1
Description: Selective ALK2 inhibitor
Chemical Name: 4-[6-[4-(1-Methylethoxy)phenyl]pyrazolo[1,5-a]pyrimidin-3-yl]-quinoline
Purity: ≥98% (HPLC)
Datasheet
Citations (8)
Literature
Pathways

Biological Activity

Selective inhibitor of the bone morphogenic protein (BMP) ALK2 receptor (IC50 = 108 nM). Exhibits no detectable inhibition of AMPK, ALK5, KDR (VEGFR-2) or PDGFRβ receptors. Blocks BMP4-induced phosphorylation of Smads 1, 5 and 8 in HEK293 cells. Promotes neurogenesis in human induced pluripotent stem cells (iPSCs) when used in combination with SB 431542 (Cat. No. 1614).

Compound Libraries

DMH-1 is also offered as part of the Tocriscreen Plus and Tocriscreen Stem Cell Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 380.44
Formula C24H20N4O
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 1206711-16-1
PubChem ID 50997747
InChI Key JMIFGARJSWXZSH-UHFFFAOYSA-N
Smiles CC(C)OC(C=C3)=CC=C3C(C=N2)=CN1C2=C(C4=CC=NC5=C4C=CC=C5)C=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.61 20
ethanol 1.9 5mM with gentle warming

Preparing Stock Solutions

The following data is based on the product molecular weight 380.44. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.63 mL 13.14 mL 26.29 mL
5 mM 0.53 mL 2.63 mL 5.26 mL
10 mM 0.26 mL 1.31 mL 2.63 mL
50 mM 0.05 mL 0.26 mL 0.53 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Bowman and Zon (2010) Swimming into the future of drug discovery: in vivo chemical screens in zebrafish. ACS Chem.Biol. 5 159 PMID: 20166761

Hao et al (2010) In vivo structure-activity relationship study of dorsomorphin analogues identifies selective VEGF and BMP inhibitors. ACS Chem.Biol. 5 245 PMID: 20020776

Neely et al (2012) DMH1, a highly selective small molecule BMP inhibitor, promotes neurogenesis of hiPSCs: comparison of PAX6 and SOX1 expression during neural induction. ACS Chem.Neurosci. 3 482 PMID: 22860217


If you know of a relevant reference for DMH-1, please let us know.

View Related Products by Product Action

View all BMP and Other Activin Receptor Inhibitors

Keywords: DMH-1, supplier, selective, activin, receptors, alk2, acvr1, bmp, type, I, bone, morphogenic, morphogenetic, protein, BMP, and, Other, Activin, Receptors, ESCs, and, iPSC, BMP, and, Other, Activin, Receptors, Tocris Bioscience

8 Citations for DMH-1

Citations are publications that use Tocris products. Selected citations for DMH-1 include:

Lu et al (2016) Generation of serotonin neurons from human pluripotent stem cells. Nat Biotechnol 34 89 PMID: 26655496

Mou et al (2016) Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells Cell Stem Cell 19 217 PMID: 27320041

Martínez et al (2015) The BMP Pathway Participates in Human Naive CD4+ T Cell Activation and Homeostasis. Cell 10 e0131453 PMID: 26110906

McDonald et al (2015) Myocardin-related transcription factor A regulates conversion of progenitors to beige adipocytes. Arthritis Res Ther 160 105 PMID: 25579684

Varas et al (2015) Blockade of bone morphogenetic protein signaling potentiates the pro-inflammatory phenotype induced by interleukin-17 and tumor necrosis factor-α combination in rheumatoid synoviocytes. Lab Chip 17 192 PMID: 26215036

Du et al (2015) Generation and expansion of highly pure motor neuron progenitors from human pluripotent stem cells. Nat.Commun. 6 6626 PMID: 25806427

Liu et al (2013) Directed differentiation of forebrain GABA interneurons from human pluripotent stem cells. Nat Protoc 8 1670 PMID: 23928500

Li et al (2013) Flow-based pipeline for systematic modulation and analysis of 3D tumor microenvironments. J Biol Chem 13 1969 PMID: 23563587


Do you know of a great paper that uses DMH-1 from Tocris? If so please let us know.

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Reviews

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Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Kinases

Kinases Product Listing

A collection of over 400 products for kinase research, the listing includes inhibitors of:

  • Receptor Tyrosine Kinases
  • Protein Kinases A, C, D and G
  • PI-3 Kinase, Akt and mTOR
  • MAPK Signaling
  • Receptor Serine/Threonine Kinases

Pathways for DMH-1

TGF-β

TGF-β Signaling Pathway

The TGF-β signaling pathway is involved in the regulation of growth and proliferation of cells along with migration, differentiation and apoptosis.

Protocols

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