Pricing Availability   Qty
Description: AMPK activator
Chemical Name: N1-(β-D-Ribofuranosyl)-5-aminoimidazole-4-carboxamide
Purity: ≥99% (HPLC)
Citations (20)
Reviews (1)
Pathways (1)

Biological Activity for AICAR

AICAR is a cell-permeable, allosteric activator of AMP-activated protein kinase (AMPK). Augments proliferation, differentiation and mineralization of osteoblastic MC3T3-EI cells and attenuates psychosine-induced expression of proinflammatory cytokines and iNOS in astrocytes. Promotes osteogenic differentiation of hAMSCs and BM-MSCs in vitro.

Compound Libraries for AICAR

AICAR is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for AICAR

M. Wt 258.23
Formula C9H14N4O5
Storage Store at -20°C
Purity ≥99% (HPLC)
CAS Number 2627-69-2
PubChem ID 17513
Smiles O[C@@H]1[C@@H](CO)O[C@@H](N2C=NC(C(N)=O)=C2N)[C@@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for AICAR

Solvent Max Conc. mg/mL Max Conc. mM
water 19.37 75
DMSO 19.37 75

Preparing Stock Solutions for AICAR

The following data is based on the product molecular weight 258.23. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.75 mM 5.16 mL 25.82 mL 51.63 mL
3.75 mM 1.03 mL 5.16 mL 10.33 mL
7.5 mM 0.52 mL 2.58 mL 5.16 mL
37.5 mM 0.1 mL 0.52 mL 1.03 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for AICAR

Certificate of Analysis / Product Datasheet
Select another batch:

References for AICAR

References are publications that support the biological activity of the product.

Giri et al (2004) 5-Aminoimidazole-4-carboxamide-1-β-4-ribofuranoside inhibits proinflammatory response in glial cells: a possible role of AMP-activated protein kinase. J.Neurosci. 24 479 PMID: 14724246

Kanazawa et al (2007) Adiponectin and AMP kinase activator stimulate proliferation, differentiation and mineralization of osteoblastic MC3T3-EI cells. BMC Cell Biol. 8 51 PMID: 18047638

Giri et al (2008) The role of AMPK in psychosine mediated effects on oligodendrocytes and astrocytes: Implication for Krabbe disease. J.Neurochem. 105 1820 PMID: 18248608

Wu et al (2011) AICAR, a small chemical molecule, primes osteogenic differentiation of adult mesenchymal stem cells. Int.J.Artif.Organs. 34 1128 PMID: 22198598

If you know of a relevant reference for AICAR, please let us know.

View Related Products by Product Action

View all AMPK Activators

Keywords: AICAR, AICAR supplier, AMPK, activators, AMP-Activated, Protein, Kinases, Enzyme, Substrates, /, Activators, Osteogenic, Stem, Cells, Mesenchymal, 2840, Tocris Bioscience

20 Citations for AICAR

Citations are publications that use Tocris products. Selected citations for AICAR include:

Mo et al (2015) Cellular energy stress induces AMPK-mediated regulation of YAP and the Hippo pathway. Nat Cell Biol 17 500 PMID: 25751140

Mohseni et al (2014) A genetic screen identifies an LKB1-MARK signalling axis controlling the Hippo-YAP pathway. PLoS One 16 108 PMID: 24362629

Bumpus and Johnson (2011) 5-Aminoimidazole-4-carboxyamide-ribonucleoside (AICAR)-stimulated hepatic expression of Cyp4a10, Cyp4a14, Cyp4a31, and other peroxisome proliferator-activated receptor α-responsive mouse genes is AICAR 5'-monophosphate-dependent and AMP-activated Proc Natl Acad Sci U S A 339 886 PMID: 21896918

Creighton et al (2011) Adenosine monophosphate-activated kinase alpha1 promotes endothelial barrier repair. FASEB J 25 3356 PMID: 21680893

Marinangeli et al (2018) AMP-Activated Protein Kinase Is Essential for the Maintenance of Energy Levels during Synaptic Activation. iScience 9 1 PMID: 30368077

Sen et al (2018) EWS-FLI1 reprograms the metabolism of Ewing sarcoma cells via positive regulation of glutamine import and serine-glycine biosynthesis. Mol Carcinog 57 1342 PMID: 29873416

Hu et al (2018) Berberine Attenuated Proliferation, Invasion and Migration by Targeting the AMPK/HNF4α/WNT5A Pathway in Gastric Carcinoma. Front Pharmacol 9 1150 PMID: 30405404

Abdulhag et al (2015) Mitochondrial complex IV deficiency, caused by mutated COX6B1, is associated with encephalomyopathy, hydrocephalus and cardiomyopathy. Eur J Hum Genet 23 159 PMID: 24781756

Mounier et al (2013) AMPKα1 regulates macrophage skewing at the time of resolution of inflammation during skeletal muscle regeneration. Cell Metab 18 251 PMID: 23931756

Fong et al (2013) Moderate excess of pyruvate augments osteoclastogenesis. Biol Open 2 387 PMID: 23616923

Hassan et al (2014) Reduced oxidative stress contributes to the lipid lowering effects of isoquercitrin in free fatty acids induced hepatocytes. Oxid Med Cell Longev 2014 313602 PMID: 25404990

Kim et al (2016) Role of Inflammatory Signaling in the Differential Effects of Saturated and Poly-unsaturated Fatty Acids on Peripheral Circadian Clocks. EBioMedicine 7 100 PMID: 27322464

Abdou et al (2014) A cell-autonomous molecular cascade initiated by AMP-activated protein kinase represses steroidogenesis. Mol Cell Biol 34 4257 PMID: 25225331

Terry et al (2012) Human kinome profiling identifies a requirement for AMP-activated protein kinase during human cytomegalovirus infection. Nat Cell Biol 109 3071 PMID: 22315427

Jurmeister et al (2014) Transcriptomic analysis reveals inhibition of androgen receptor activity by AMPK in prostate cancer cells. Oncotarget 5 3785 PMID: 25003216

Seto et al (2013) Acute SimV. inhibits K ATP channels of porcine coronary artery myocytes. FASEB J 8 e66404 PMID: 23799098

Taub et al (2013) Distinct energy requirements for human memory CD4 T-cell homeostatic functions. BMC Cancer 27 342 PMID: 22972918

Yung et al (2013) Activation of AMPK inhibits cervical cancer cell growth through AKT/FOXO3a/FOXM1 signaling cascade. Stem Cells Transl Med 13 327 PMID: 23819460

Hutchins et al (2013) Calcium release-dependent actin flow in the leading process mediates axophilic migration. J Pharmacol Exp Ther 33 11361 PMID: 23843509

Hashiguchi (2017) Direct versus indirect actions of ghrelin on hypothalamic NPY neurons. PLoS One 12 e0184261 PMID: 28877214

Do you know of a great paper that uses AICAR from Tocris? Please let us know.

Reviews for AICAR

Average Rating: 4 (Based on 1 Review.)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used AICAR?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

good product, satisfied.
By Kishor Pant on 05/22/2019
Assay Type: In Vitro
Species: Human

Cancer and normal cell lines treated with 2mM for different times and we noticed modulation in the AMPK and p53 expression.

review image

Pathways for AICAR

AMPK Signaling Pathway

AMPK Signaling Pathway

AMPK signaling pathway plays an important role in the cellular reponse to low levels of available ATP, often caused by stresses such as heat shock, ischemia or hypoxia.