TSA Vivid Fluorophore Kit 520

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Cat.No. 7523 - TSA Vivid Fluorophore Kit 520
Description: Signal amplification kit for use in ISH, ICC and IHC
Datasheet
Citations
Reviews
Protocols (1)

Biological Activity for TSA Vivid Fluorophore Kit 520

TSA Vivid reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. TSA Vivid reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAScope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.

The basis for signal amplification comes from the specific and high-density deposition of TSA Vivid dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).

Excitation maximum = 494 nm; emission maximum = 517 nm

Please see the product datasheet or protocol for information on product use.

Optical Data for TSA Vivid Fluorophore Kit 520

Spectral data for TSA Vivid Fluorophore kit 520 Emission Color Green
λabs 494 nm
λem 517 nm

Application in RNAscope™ Multiplex Fluorescent v2 Assay

RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with the leading competitor dye (left) and TSA Vivid dye 520 (right). Dye used at 1:1500 dilution. Marker shown is Polr2a in green. Nuclei are counterstained with DAPI (blue, Cat. No. 5748, Tocris).

3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells

Technical Data for TSA Vivid Fluorophore Kit 520

Storage Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for TSA Vivid Fluorophore Kit 520

References for TSA Vivid Fluorophore Kit 520

References are publications that support the biological activity of the product.

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Citations for TSA Vivid Fluorophore Kit 520

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Protocols for TSA Vivid Fluorophore Kit 520

The following protocol features additional information for the use of TSA Vivid Fluorophore Kit 520 (Cat. No. 7523).