Cyanine 3 Tyramide

Pricing Availability   Qty
Description: Orange fluorescent reagent widely used for signal amplification in IHC and FISH
Chemical Name: 2-[3-[(1-Ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino-6-oxohexyl]-3,3-dimethyl-5-sulfo-3H-indolium inner salt
Purity: ≥95% (HPLC)

Biological Activity for Cyanine 3 Tyramide

Cyanine 3 Tyramide is an orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

Optical Data for Cyanine 3 Tyramide

Cyanine 3 Tyramide Dye Spectra
Emission Color Orange
λabs 550 nm
λem 563 nm
Extinction Coefficient (ε) 150000 M-1cm-1
Quantum Yield (φ) 0.31
Closest Laser line 532 nm
Application Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH

Plan Your Experiments

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Technical Data for Cyanine 3 Tyramide

M. Wt 749.94
Formula C39H47N3O8S2
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 174961-75-2
Smiles CCN1C2=C(C=C(C=C2)S(O)(=O)=O)C(/C1=C\C=C\C3=[N+](CCCCCC(NCCC4=CC=C(O)C=C4)=O)C5=C(C=C(C=C5)S([O-])(=O)=O)C3(C)C)(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Cyanine 3 Tyramide

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 12 16

Preparing Stock Solutions for Cyanine 3 Tyramide

The following data is based on the product molecular weight 749.94. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.16 mM 8.33 mL 41.67 mL 83.34 mL
0.8 mM 1.67 mL 8.33 mL 16.67 mL
1.6 mM 0.83 mL 4.17 mL 8.33 mL
8 mM 0.17 mL 0.83 mL 1.67 mL

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Product Datasheets for Cyanine 3 Tyramide

Keywords: Cyanine 3 Tyramide, Cyanine 3 Tyramide supplier, C3, tyramide, signal, amplification, TSA, horseradish, peroxidase, HRP, orange, Tyramide, Signaling, Amplification, (TSA), 6457, Tocris Bioscience

Citations for Cyanine 3 Tyramide

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