Biotinyl Tyramide

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Cat.No. 6241 - Biotinyl Tyramide | C18H25N3O3S | CAS No. 41994-02-9
Description: Reagent widely used for signal amplification in IHC and ISH
Chemical Name: (3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature

Biological Activity

Reagent widely used for signal amplification in IHC and in situ hybridization (ISH). HRP catalyzes localized deposition of multiple biotinyl tyramide molecules (catalyzed reporter deposition, CARD), which can be detected using a labeled streptavidin conjugate. The localization of deposition around HRP leads to good resolution. Suitable for either fluorescence or chromogenic detection.

Technical Data

M. Wt 363.47
Formula C18H25N3O3S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 41994-02-9
PubChem ID 21826423
InChI Key VZWXNOBHWODXCW-ZOBUZTSGSA-N
Smiles [H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCC3=CC=C(C=C3)O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 36.35 100

Preparing Stock Solutions

The following data is based on the product molecular weight 363.47. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.75 mL 13.76 mL 27.51 mL
5 mM 0.55 mL 2.75 mL 5.5 mL
10 mM 0.28 mL 1.38 mL 2.75 mL
50 mM 0.06 mL 0.28 mL 0.55 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Bobrow et al (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 125 279 PMID: 2558138

McKay et al (1997) Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide. Mol.Pathol. 50 322 PMID: 9536283


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Keywords: Biotinyl Tyramide, supplier, in, situ, hybridization, ISH, immunohistochemistry, IHC, fluorescence, tyramide, signal, amplification, Biochemicals, and, Molecular, Biology, Biochemicals, and, Molecular, Biology, Tocris Bioscience

Citations for Biotinyl Tyramide

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Literature in this Area

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