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Reagent widely used for signal amplification in IHC and in situ hybridization (ISH). HRP catalyzes localized deposition of multiple biotinyl tyramide molecules (catalyzed reporter deposition, CARD), which can be detected using a labeled streptavidin conjugate. The localization of deposition around HRP leads to good resolution. Suitable for either fluorescence or chromogenic detection.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 363.47. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.75 mL||13.76 mL||27.51 mL|
|5 mM||0.55 mL||2.75 mL||5.5 mL|
|10 mM||0.28 mL||1.38 mL||2.75 mL|
|50 mM||0.06 mL||0.28 mL||0.55 mL|
References are publications that support the biological activity of the product.
Bobrow et al (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 125 279 PMID: 2558138
McKay et al (1997) Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide. Mol.Pathol. 50 322 PMID: 9536283
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Keywords: Biotinyl Tyramide, Biotinyl Tyramide supplier, in, situ, hybridization, ISH, immunohistochemistry, IHC, fluorescence, tyramide, signal, amplification, Biochemicals, and, Molecular, Biology, 6241, Tocris Bioscience
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BP-labelling experiment in SH-SY5Y cells stably expressing MTS-APEX2. Tested range 500-1000µM biotinyl tyramide.
We use biotinyl tyramide to biotinylate cells - we have done this successfully without any issues to date. Biotinylated proteins can be detected by western blot using a streptavidin conjugated antibody (see image)
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