Biotinyl Tyramide

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Cat.No. 6241 - Biotinyl Tyramide | C18H25N3O3S | CAS No. 41994-02-9
Description: Reagent widely used for signal amplification in IHC and FISH
Chemical Name: (3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews (2)
Literature (1)

Biological Activity

Reagent widely used for signal amplification in IHC and fluorescent in situ hybridization (ISH). HRP catalyzes localized deposition of multiple biotinyl tyramide molecules (catalyzed reporter deposition, CARD), which can be detected using a labeled streptavidin conjugate. The localization of deposition around HRP leads to good resolution. Suitable for either fluorescence or chromogenic detection.

Technical Data

M. Wt 363.47
Formula C18H25N3O3S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 41994-02-9
PubChem ID 21826423
InChI Key VZWXNOBHWODXCW-ZOBUZTSGSA-N
Smiles [H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCC3=CC=C(C=C3)O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 36.35 100

Preparing Stock Solutions

The following data is based on the product molecular weight 363.47. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.75 mL 13.76 mL 27.51 mL
5 mM 0.55 mL 2.75 mL 5.5 mL
10 mM 0.28 mL 1.38 mL 2.75 mL
50 mM 0.06 mL 0.28 mL 0.55 mL

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Bobrow et al (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 125 279 PMID: 2558138

McKay et al (1997) Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide. Mol.Pathol. 50 322 PMID: 9536283


If you know of a relevant reference for Biotinyl Tyramide, please let us know.

Keywords: Biotinyl Tyramide, Biotinyl Tyramide supplier, in, situ, hybridization, ISH, immunohistochemistry, IHC, fluorescence, fluorescent, tyramide, signal, amplification, Tyramide, Signaling, Amplification, (TSA), 6241, Tocris Bioscience

Citations for Biotinyl Tyramide

Citations are publications that use Tocris products.

Currently there are no citations for Biotinyl Tyramide. Do you know of a great paper that uses Biotinyl Tyramide from Tocris? Please let us know.

Reviews for Biotinyl Tyramide

Average Rating: 5 (Based on 2 Reviews.)

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Biotinyl tyramide used successfully for BP-labelling of mitochondrial proteins.
By Anonymous on 02/12/2020
Assay Type: In Vitro
Species: Human

BP-labelling experiment in SH-SY5Y cells stably expressing MTS-APEX2. Tested range 500-1000µM biotinyl tyramide.

review image

Great product for Biotinylation.
By Anonymous on 02/07/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: SH-SY5Y

We use biotinyl tyramide to biotinylate cells - we have done this successfully without any issues to date. Biotinylated proteins can be detected by western blot using a streptavidin conjugated antibody (see image)

review image

Literature in this Area

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