Fluorescein Tyramide

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Cat.No. 6456 - Fluorescein Tyramide | C29H21NO7 | CAS No. 210236-90-1
Description: Green fluorescent reagent widely used for signal amplification in IHC and ISH
Chemical Name: 3',6'-Dihydroxy-N-(4-hydroxyphenylethyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5(6)-carboxamide
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity

Green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, ISH, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

Optical Data for Fluorescein Tyramide

Emission Color Green
λabs 494 nm
λem 517 nm
Extinction Coefficient (ε) 70000 M-1cm-1
Closest Laser line 488 nm
Application Tyramide signal amplification (TSA) in IHC, ICC, ISH, FISH

Technical Data

M. Wt 495.48
Formula C29H21NO7
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 210236-90-1
InChI Key JZSWAHXXTWIPFB-UHFFFAOYSA-N
Smiles OC1=CC2=C(C3(C4=CC=C(C=C4O2)O)C5=CC=C(C=C5C(O3)=O)C(NCCC6=CC=C(C=C6)O)=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.89 24

Preparing Stock Solutions

The following data is based on the product molecular weight 495.48. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.24 mM 8.41 mL 42.05 mL 84.09 mL
1.2 mM 1.68 mL 8.41 mL 16.82 mL
2.4 mM 0.84 mL 4.2 mL 8.41 mL
12 mM 0.17 mL 0.84 mL 1.68 mL

Molarity Calculator

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Reconstitution Calculator

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References

References are publications that support the biological activity of the product.

Karkmann et al (1999) Enzymatic signal amplification for sensitive detection of intracellular antigens by flow cytometry. J.Immunol.Methods 230 113 PMID: 10594358

Schriml et al (1999) Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 27 608 PMID: 10489619

Hopman et al (1998) Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification. J.Histochem.Cytochem. 46 771 PMID: 9603790

van Gijlswijk et al (1997) Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J.Histochem.Cytochem. 45 375 PMID: 9071319

Bobrow et al (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 125 279 PMID: 2558138


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Citations for Fluorescein Tyramide

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