Cyanine 5 Tyramide

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Cat.No. 6458 - Cyanine 5 Tyramide | C41H49N3O8S2 | CAS No. 1431148-26-3
Description: Red fluorescent reagent widely used for signal amplification in IHC and ISH
Alternative Names: Cy5 Tyramide
Chemical Name: 2-[5-[1,3-Dihydro-1-[6-[[(2-(4-hydroxyphenyl)ethyl]amino-6-oxohexyl]3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium inner salt
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity

Red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, ISH, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

Optical Data for Cyanine 5 Tyramide

Emission Color Red
λabs 651 nm
λem 665 nm
Extinction Coefficient (ε) 250000 M-1cm-1
Closest Laser line 647 nm
Application Tyramide signal amplification (TSA) in IHC, ICC, ISH, FISH

Technical Data

M. Wt 775.97
Formula C41H49N3O8S2
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 1431148-26-3
PubChem ID 135290897
InChI Key NHSJCIBSNKLAMA-UHFFFAOYSA-N
Smiles CC[N+]1=C(C(C)(C2=CC(S([O-])(=O)=O)=CC=C12)C)/C=C/C=C/C=C3N(C4=CC=C(S(O)(=O)=O)C=C4C/3(C)C)CCCCCC(NCCC5=CC=C(C=C5)O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.64 15

Preparing Stock Solutions

The following data is based on the product molecular weight 775.97. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.15 mM 8.59 mL 42.96 mL 85.91 mL
0.75 mM 1.72 mL 8.59 mL 17.18 mL
1.5 mM 0.86 mL 4.3 mL 8.59 mL
7.5 mM 0.17 mL 0.86 mL 1.72 mL

Molarity Calculator

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References

References are publications that support the biological activity of the product.

Chanda et al (2017) Identification of the SUMO E3 ligase PIAS1 as a potential survival biomarker in breast cancer. PLoS One 12 e0177639 PMID: 28493978

Matsumoto et al (2017) Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer. Br.J.Cancer 117 1537 PMID: 28881356

Wong et al (2001) Identification of differentially expressed genes from ovarian cancer cells by MICROMAX cDNA microarray system. Biotechniques 30 670 PMID: 11252802


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Keywords: Cyanine 5 Tyramide, Cyanine 5 Tyramide supplier, Cy5, tyramide, signal, amplification, TSA, horseradish, peroxidase, HRP, red, Tyramide, Biochemicals, and, Molecular, Biology, Other, Fluorescent, Probes, 6458, Tocris Bioscience

Citations for Cyanine 5 Tyramide

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