- Cell Biology
- Product Type
- Research Area
- New Products
- About Tocris
- Contact Us
Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. It catalyzes tetrahydrofolate regeneration by reduction of dihydrofolate, using NADPH as a cofactor. DHFR has a key role in cell growth and proliferation.
|Cat. No.||Product Name / Activity|
|Dihydrofolate reductase inhibitor|
|High affinity dihydrofolate reductase inhibitor; also inhibits thymidylate synthase, GARFT and AICART|
|Photoswitchable inhibitor of human dihydrofolate reductase|
|DHFR inhibitor; also potent inhibitor of multidrug and toxin extrusion (MATE) transporters and STAT3 inhibitor|
|1592||SCH 79797 dihydrochloride|
|Inhibits DHFR; also potent and selective PAR1 antagonist; broad spectrum antibiotic|
Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. It catalyzes tetrahydrofolate regeneration by reduction of dihydrofolate, using NADPH as a cofactor. Tetrahydrofolate is essential for de novo purine and thymidylate synthesis, hence DHFR has a critical role in cell growth and proliferation.
DHFR is the target for many cancer therapies including methotrexate because it is a key enzyme in cell replication. Basal and regulated DHFR gene expression is controlled by Sp1 and E2F family transcription factors respectively and levels of this enzyme peak at the G1/S cell cycle boundary. Autoregulation, through DHFR-RNA interactions, has also been reported. The human gene encoding this enzyme is localized to 5q11.2-13.2.