Irreversible inhibitor of lysine-specific demethylase 1 (LSD1/BHC110) and monoamine oxidase (MAO). Inhibits histone demethylation. In combination with CHIR 99021 (Cat. No. 4423), enables reprogramming of mouse embryonic fibroblasts transduced by only two factors, Oct4 and Klf4, into induced pluripotent stem (iPS) cells.
Tranylcypromine hydrochloride is also offered as part of the Tocriscreen Plus, Tocriscreen Epigenetics Toolbox, Tocriscreen Library of FDA-Approved Compounds and Tocriscreen Stem Cell Toolbox. Find out more about compound libraries available from Tocris.
|Storage||Desiccate at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 169.65. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||5.89 mL||29.47 mL||58.94 mL|
|5 mM||1.18 mL||5.89 mL||11.79 mL|
|10 mM||0.59 mL||2.95 mL||5.89 mL|
|50 mM||0.12 mL||0.59 mL||1.18 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Lee et al (2006) Histone H3 lysine 4 demethylation is a target of nonselective antidepressive medications. Chem.Biol. 13 563 PMID: 16793513
Schmidt and McCafferty (2007) trans-2-Phenylcyclopropylamine is a mechanism-based inactivator of the histone demethylase LSD1. Biochemistry 46 4408 PMID: 17367163
Li et al (2009) Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. Stem Cells 27 2992 PMID: 19839055
If you know of a relevant reference for Tranylcypromine hydrochloride, please let us know.
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Keywords: Tranylcypromine hydrochloride, supplier, irreversible, inhibitors, inhibits, monoamine, oxidase, A, B, MAOA, MAOB, lysine-specific, demethylase, 1, LSD1, BCH110, histone, demethylases, demethylation, oxygenases, oxidases, epigenetics, stem, cells, pluripotency, pluripotent, oct4, KDM1A, 2-PCPA, Histone, Demethylases, Tocris Bioscience
3 Citations for Tranylcypromine hydrochloride
Citations are publications that use Tocris products. Selected citations for Tranylcypromine hydrochloride include:
Li et al (2011) Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules. Cell 21 196 PMID: 20956998
Zhang et al (2016) Pharmacological reprogramming of fibroblasts into neural stem cells by signaling-directed transcriptional activation. Cell Stem Cell 18 653 PMID: 27133794
Kearns et al (2015) Functional annotation of native enhancers with a Cas9-histone demethylase fusion. Nat Methods 12 401 PMID: 25775043
Do you know of a great paper that uses Tranylcypromine hydrochloride from Tocris? If so please let us know.
Literature in this Area
Epigenetics Scientific Review
Written by Susanne Müller-Knapp and Peter J. Brown, this review gives an overview of the development of chemical probes for epigenetic targets, as well as the impact of these tool compounds being made available to the scientific community. In addition, their biological effects are also discussed. Epigenetic compounds available from Tocris are listed.
Epigenetics Research Bulletin
Produced by Tocris and updated in 2014, the epigenetics research bulletin gives an introduction into mechanisms of epigenetic regulation, and highlights key Tocris products for epigenetics targets including:
- DNA Methyltransferases
- Histone Deacetylases
- Histone Demethylases
- Histone Methyltransferases