Pricing Availability   Qty
Description: Thyroid hormone; also promotes differentiation of oligodendroglial precursor cells
Chemical Name: O-(4-Hydroxy-3-iodophenyl-3,5-diiodo-L-tyrosine
Purity: ≥98% (HPLC)
Protocols (1)

Biological Activity for T3

T3 is a thyroid hormone. Promotes differentiation of oligodendroglial precursor cells in neonatal rats and promotes maturation of hiPSC-derived cardiomyocytes. Also specifies cone cell subtype in retinal cell differentiation protocols. T3 is used in protocols to generate cortical spheroids from hPSCs.

T3 synthesized to Ancillary Material Grade also available.

For more information about how T3 may be used, see our protocol: Generation of β cells from hPSCs

Technical Data for T3

M. Wt 650.97
Formula C15H12I3NO4
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 6893-02-3
PubChem ID 5920
Smiles N[C@H](C(O)=O)CC1=CC(I)=C(C(I)=C1)OC2=CC(I)=C(C=C2)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for T3

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 32.55 50

Preparing Stock Solutions for T3

The following data is based on the product molecular weight 650.97. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 3.07 mL 15.36 mL 30.72 mL
2.5 mM 0.61 mL 3.07 mL 6.14 mL
5 mM 0.31 mL 1.54 mL 3.07 mL
25 mM 0.06 mL 0.31 mL 0.61 mL

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Product Datasheets for T3

Certificate of Analysis / Product Datasheet
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References for T3

References are publications that support the biological activity of the product.

Marta et al (1998) Sustained neonatal hyperthyroidism in the rat affects myelination in the central nervous system. J.Neurosci.Res. 53 251 PMID: 9671982

Madhavan et al (2018) Induction of myelinating oligodendrocytes in human cortical spheroids. Nat Methods. 15 700 PMID: 30046099

Yang et al (2014) Tri-iodo-L-thyronine promotes the maturation of human cardiomyocytes-derived from induced pluripotent stem cells. J.Mol.CellCardiol. 72 296 PMID: 24735830

Eldred et al (2018) Thyroid hormone signaling specifies cone subtypes in human retinal organoids. Science 362 eaau6348 PMID: 30309916

Surendran et al (2022) An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells. STAR Protoc. 3 101803 PMID: 36386870

If you know of a relevant reference for T3, please let us know.

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Citations for T3

Citations are publications that use Tocris products.

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Protocols for T3

The following protocol features additional information for the use of T3 (Cat. No. 6666).