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Submit ReviewSCOTfluor glucose probe 510
Description: Fluorescent glucose probe
Chemical Name: 2-Deoxy-2-[(7-nitro-2,1,3-benzoselenadiazol-4-yl)amino]-D-glucose
Purity: ≥98% (HPLC)
Biological Activity for SCOTfluor glucose probe 510
SCOTfluor glucose probe 510 is a fluorescent probe for visualizing glucose uptake in vivo. It can be multiplexed with BFP and GFP. Excitation and emission maxima (λ) are 490 and 605 nm, respectively.
Scientific Data
Confocal Microscopy
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Application of SCOTfluor glucose probe 510 in HeLa cells. Fluorescence microscopy image of GLUT4-EGFP HeLa cells treated with SCOTfluor glucose probe 510 (100 μm). Colors: green (GLUT4-EGFP), red (SCOTfluor glucose probe 510), blue: Hoechst 33342 (Catalog # 5117). Image kindly provided by Prof. Marc Vendrell, acquired by Dr Sam Benson, Centre for Inflammation Research, University of Edinburgh.
In vivo Imaging
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Application of SCOTfluor glucose probe 510 in zebrafish embryos. In vivo images of zebrafish embryos (28 h post fertilization) after injection of SCOTfluor glucose probe 510 (50 pmol) to the yolk sac in wildtype zebrafish (left) or zebrafish injected at one cell stage with 4.2 ng anti-sense glu2 morpholino (right). Image kindly provided by Prof. Marc Vendrell, acquired by Dr Yi Feng, Centre for Inflammation Research, University of Edinburgh.
Licensing Information
Sold under license from the University of Edinburgh
Optical Data for SCOTfluor glucose probe 510
| Emission Color | Orange |
|---|---|
| λabs | 490 nm |
| λem | 605 nm |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Fluorescence Microscopy, Confocal Microscopy, in vivo Imaging |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for SCOTfluor glucose probe 510
| M. Wt | 405.23 |
| Formula | C12H14N4O7Se |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 2490298-72-9 |
| PubChem ID | 155694629 |
| InChI Key | LXWDEVJSKWMYFZ-BGCUHRRXSA-N |
| Smiles | OC[C@H]1OC([C@@H]([C@H]([C@@H]1O)O)NC2=CC=C(N(=O)=O)C3=N[Se]N=C23)O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for SCOTfluor glucose probe 510
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.05 | 10 |
Preparing Stock Solutions for SCOTfluor glucose probe 510
The following data is based on the product molecular weight 405.23. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 24.68 mL | 123.39 mL | 246.77 mL |
| 0.5 mM | 4.94 mL | 24.68 mL | 49.35 mL |
| 1 mM | 2.47 mL | 12.34 mL | 24.68 mL |
| 5 mM | 0.49 mL | 2.47 mL | 4.94 mL |
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Product Datasheets for SCOTfluor glucose probe 510
References for SCOTfluor glucose probe 510
References are publications that support the biological activity of the product.
Benson et al (2019) SCOTfluors: small, conjugatable, orthogonal, and tunable fluorophores for in vivo imaging of cell metabolism. Angew.Chem.Int.Ed.Engl. 58 6911 PMID: 30924239
Benson et al (2021) Photoactivatable metabolic warheads enable precise and safe ablation of target cells in vivo. Nat.Commun. 12 2369 PMID: 33888691
If you know of a relevant reference for SCOTfluor glucose probe 510, please let us know.
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Citations for SCOTfluor glucose probe 510
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