Hoechst 33342

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Cat.No. 5117 - Hoechst 33342 | C27H28N6O.3HCl | CAS No. 23491-52-3
Description: Fluorescent dye for labeling DNA
Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations (3)
Reviews (2)
Literature (1)

Biological Activity

Cell permeable fluorescent DNA stain; binds minor groove of AT-rich regions. Used to quantify DNA in viable cells.

Technical Data

M. Wt 561.93
Formula C27H28N6O.3HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 23491-52-3
PubChem ID 16760503
InChI Key JABNPSKWVNCGMX-UHFFFAOYSA-N
Smiles CN(CC2)CCN2C1=CC(N=C(C4=CC(NC(C6=CC=C(OCC)C=C6)=N5)=C5C=C4)N3)=C3C=C1.Cl.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 28.1 50
DMSO 28.1 50

Preparing Stock Solutions

The following data is based on the product molecular weight 561.93. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 3.56 mL 17.8 mL 35.59 mL
2.5 mM 0.71 mL 3.56 mL 7.12 mL
5 mM 0.36 mL 1.78 mL 3.56 mL
25 mM 0.07 mL 0.36 mL 0.71 mL

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Portugal and Waring (1988) Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochim.Biophys.Acta. 949 158 PMID: 2449244

Loken (1980) Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry 1 136 PMID: 7028425


If you know of a relevant reference for Hoechst 33342, please let us know.

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Keywords: Hoechst 33342, Hoechst 33342 supplier, DNA, stains, fluorescent, dyes, nucleic, acid, cell, permeable, Fluorescent, Stains, 5117, Tocris Bioscience

3 Citations for Hoechst 33342

Citations are publications that use Tocris products. Selected citations for Hoechst 33342 include:

Yang et al (2019) SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Autophagy 1 PMID: 30871407

Durymanov et al (2019) Subcutaneous Inoculation of 3D Pancreatic Cancer Spheroids Results in Development of Reproducible Stroma-Rich Tumors. Transl Oncol 12 180 PMID: 30554606

Tabdanov et al (2018) Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing. Cell Rep 25 328 PMID: 30304674


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Reviews for Hoechst 33342

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Staining nuclei.
By Anonymous on 10/27/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: PC3

Add Hoechst dyes at 1 ug/mL.Incubate cells at room temperature for 10 minutes.


Nuclear labeling.
By Anonymous on 11/01/2017
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK 293

Immunostaining of HEK 293 cells

Works well at 1:2500 dilution incubated for 20 minutes at room temperature just before mounting the coverslips with the mounting media

review image

Literature in this Area

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