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Hoechst 33342 stains and binds minor groove of AT-rich regions. Used to quantify DNA in viable cells. Cell permeable fluorescent DNA dye.
Optical Data for Hoechst 33342
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 561.93. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.5 mM||3.56 mL||17.8 mL||35.59 mL|
|2.5 mM||0.71 mL||3.56 mL||7.12 mL|
|5 mM||0.36 mL||1.78 mL||3.56 mL|
|25 mM||0.07 mL||0.36 mL||0.71 mL|
References are publications that support the biological activity of the product.
Portugal and Waring (1988) Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochim.Biophys.Acta. 949 158 PMID: 2449244
Loken (1980) Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry 1 136 PMID: 7028425
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3 Citations for Hoechst 33342
Citations are publications that use Tocris products. Selected citations for Hoechst 33342 include:
Yang et al (2019) SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Autophagy 1 PMID: 30871407
Durymanov et al (2019) Subcutaneous Inoculation of 3D Pancreatic Cancer Spheroids Results in Development of Reproducible Stroma-Rich Tumors. Transl Oncol 12 180 PMID: 30554606
Tabdanov et al (2018) Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing. Cell Rep 25 328 PMID: 30304674
Do you know of a great paper that uses Hoechst 33342 from Tocris? Please let us know.
Reviews for Hoechst 33342
Average Rating: 5 (Based on 3 Reviews.)
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I used this dye to stain the nucleus of live cells. After making the stock solution, diluted the dye 1:1000 ratio in the culture media/PBS and added to cells and incubated for 5 mins.
Add Hoechst dyes at 1 ug/mL.Incubate cells at room temperature for 10 minutes.
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