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Submit ReviewHoechst 33342
Description: Blue-fluorescent dye for DNA staining. Cell permeable. Used for: nuclear counterstain, apoptosis analysis, fixed and live-cell staining. Application: flow cytometry, confocal microscopy
Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
Purity: ≥98% (HPLC)
Biological Activity for Hoechst 33342
Key information: Hoechst 33342 is a blue-fluorescent dye for DNA staining. Cell permeable. Suitable for fixed and live-cell staining. Commonly used as a counterstain in fluorescence microscopy.
Used for: nuclear counterstain, DNA visualization, cell cycle studies, apoptosis analysis.
Application: flow cytometry, confocal microscopy.
Properties and Photophysical Data: Hoechst 33342 binds to the AT-rich regions of the minor grove in DNA which renders it specific for nuclear chromatin. Excitation and emission maxima (λ) are 350 nm and 461 nm, respectively.
Scientific Data
Fluorescence Imaging
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Hoechst 33342 | CAS No. 875756-97-1 Fluorescent dye for staining DNA
Optical Data for Hoechst 33342
| Emission Color | Blue |
|---|---|
| λabs | 350 nm |
| λem | 461 nm |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Confocal Microscopy |
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Hoechst 33342
| M. Wt | 561.93 |
| Formula | C27H28N6O.3HCl |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 875756-97-1 |
| PubChem ID | 16760503 |
| InChI Key | JABNPSKWVNCGMX-UHFFFAOYSA-N |
| Smiles | CN(CC2)CCN2C1=CC(N=C(C4=CC(NC(C6=CC=C(OCC)C=C6)=N5)=C5C=C4)N3)=C3C=C1.Cl.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Hoechst 33342
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 28.1 | 50 | |
| DMSO | 28.1 | 50 |
Preparing Stock Solutions for Hoechst 33342
The following data is based on the product molecular weight 561.93. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.56 mL | 17.8 mL | 35.59 mL |
| 2.5 mM | 0.71 mL | 3.56 mL | 7.12 mL |
| 5 mM | 0.36 mL | 1.78 mL | 3.56 mL |
| 25 mM | 0.07 mL | 0.36 mL | 0.71 mL |
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Product Datasheets for Hoechst 33342
References for Hoechst 33342
References are publications that support the biological activity of the product.
Portugal and Waring (1988) Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochim.Biophys.Acta. 949 158 PMID: 2449244
Loken (1980) Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry 1 136 PMID: 7028425
If you know of a relevant reference for Hoechst 33342, please let us know.
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12 Citations for Hoechst 33342
Citations are publications that use Tocris products. Selected citations for Hoechst 33342 include:
Tabdanov et al (2018) Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing. Cell Rep 25 328 PMID: 30304674
Yang et al (2019) SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Autophagy 1 PMID: 30871407
Durymanov et al (2019) Subcutaneous Inoculation of 3D Pancreatic Cancer Spheroids Results in Development of Reproducible Stroma-Rich Tumors. Transl Oncol 12 180 PMID: 30554606
Mária et al (2021) Involvement of P2Y12 receptors in a nitroglycerin-induced model of migraine in male mice. Br J Pharmacol 178 4626-4645 PMID: 34363208
Steve R et al (2021) Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells. Cancers (Basel) 13 PMID: 34831010
Jian et al (2021) Mechanical Counterbalance of Kinesin and Dynein Motors in a Microtubular Network Regulates Cell Mechanics, 3D Architecture, and Mechanosensing. ACS Nano 15 17528-17548 PMID: 34677937
Mike et al (2022) Receptor Interactome Discovery with RDIMIS, a Membrane Protein Interaction Screen Using Recombinant Extracellular Vesicles. Curr Protoc 2 e483 PMID: 35822836
Chayanon et al (2022) Methods for mitochondrial health assessment by High Content Imaging System. MethodsX 9 101685 PMID: 35464807
Diana et al (2022) A Multiscale Study of Phosphorylcholine Driven Cellular Phenotypic Targeting. ACS Cent Sci 8 891-904 PMID: 35912343
Jie et al (2019) GORASP2/GRASP55 collaborates with the PtdIns3K UVRAG complex to facilitate autophagosome-lysosome fusion. Autophagy 15 1787-1800 PMID: 30894053
Paolo P et al (2020) Engineering Elastic Nano- and Micro-Patterns and Textures for Directed Cell Motility. STAR Protoc 1 PMID: 32734278
Ke-Fang et al (2023) A Simple and Affordable Method to Create Nonsense Mutation Clones of p53 for Studying the Premature Termination Codon Readthrough Activity of PTC124. Biomedicines 11 PMID: 37238980
Do you know of a great paper that uses Hoechst 33342 from Tocris? Please let us know.
Reviews for Hoechst 33342
Average Rating: 5 (Based on 3 Reviews.)
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HCT116 spheroids.
By
Gabriele Strusi on 12/12/2024
Assay Type: In Vitro
Species: Human
HCT116 spheroids stained with 7 um Hoechst 33342
MCF7 spheroids.
By
Caterina Suelzu on 12/12/2024
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: MCF7
MCF7 spheroids stained with 7 uM Hoechst 33342
Nuclear labeling.
By
Anonymous on 11/01/2017
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK 293
Immunostaining of HEK 293 cells
Works well at 1:2500 dilution incubated for 20 minutes at room temperature just before mounting the coverslips with the mounting media
Literature in this Area
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Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
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