Hoechst 33342

Pricing Availability   Qty
Description: Fluorescent dye for staining DNA
Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
Purity: ≥98% (HPLC)
Citations (12)
Reviews (1)
Literature (1)

Biological Activity for Hoechst 33342

Hoechst 33342 stains and binds minor groove of AT-rich regions. Used to quantify DNA in viable cells. Cell permeable fluorescent DNA dye.

Scientific Data

Fluorescence Imaging Fluorescent dye for staining DNA View Larger

Hoechst 33342 | CAS No. 875756-97-1 Fluorescent dye for staining DNA

Optical Data for Hoechst 33342

Hoechst 33342 Dye Spectra
Emission Color Blue
λabs 350 nm
λem 461 nm
Cell Permeable Yes
Application Flow Cytometry, Confocal Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for Hoechst 33342

M. Wt 561.93
Formula C27H28N6O.3HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 875756-97-1
PubChem ID 16760503
Smiles CN(CC2)CCN2C1=CC(N=C(C4=CC(NC(C6=CC=C(OCC)C=C6)=N5)=C5C=C4)N3)=C3C=C1.Cl.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Hoechst 33342

Solvent Max Conc. mg/mL Max Conc. mM
water 28.1 50
DMSO 28.1 50

Preparing Stock Solutions for Hoechst 33342

The following data is based on the product molecular weight 561.93. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 3.56 mL 17.8 mL 35.59 mL
2.5 mM 0.71 mL 3.56 mL 7.12 mL
5 mM 0.36 mL 1.78 mL 3.56 mL
25 mM 0.07 mL 0.36 mL 0.71 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Hoechst 33342

Certificate of Analysis / Product Datasheet
Select another batch:

References for Hoechst 33342

References are publications that support the biological activity of the product.

Portugal and Waring (1988) Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochim.Biophys.Acta. 949 158 PMID: 2449244

Loken (1980) Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry 1 136 PMID: 7028425

If you know of a relevant reference for Hoechst 33342, please let us know.

Keywords: Hoechst 33342, Hoechst 33342 supplier, hoechst, staining, dye, DNA, stains, fluorescent, dyes, nucleic, acid, cell, permeable, counterstain, Fluorescent, Stains, Cell, Viability, and, Dyes, Counterstains, Flow, Cytometry, 5117, Tocris Bioscience

12 Citations for Hoechst 33342

Citations are publications that use Tocris products. Selected citations for Hoechst 33342 include:

Mária et al (2021) Involvement of P2Y12 receptors in a nitroglycerin-induced model of migraine in male mice. Br J Pharmacol 178 4626-4645 PMID: 34363208

Steve R et al (2021) Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells. Cancers (Basel) 13 PMID: 34831010

Jian et al (2021) Mechanical Counterbalance of Kinesin and Dynein Motors in a Microtubular Network Regulates Cell Mechanics, 3D Architecture, and Mechanosensing. ACS Nano 15 17528-17548 PMID: 34677937

Tabdanov et al (2018) Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing. Cell Rep 25 328 PMID: 30304674

Do you know of a great paper that uses Hoechst 33342 from Tocris? Please let us know.

Reviews for Hoechst 33342

Average Rating: 5 (Based on 1 Review.)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Hoechst 33342?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Nuclear labeling.
By Anonymous on 11/01/2017
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK 293

Immunostaining of HEK 293 cells

Works well at 1:2500 dilution incubated for 20 minutes at room temperature just before mounting the coverslips with the mounting media

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals