Hoechst 33342
Biological Activity
Cell permeable fluorescent DNA stain; binds minor groove of AT-rich regions. Used to quantify DNA in viable cells.
Technical Data
| M. Wt | 561.93 |
| Formula | C27H28N6O.3HCl |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 23491-52-3 |
| PubChem ID | 16760503 |
| InChI Key | JABNPSKWVNCGMX-UHFFFAOYSA-N |
| Smiles | CN(CC2)CCN2C1=CC(N=C(C4=CC(NC(C6=CC=C(OCC)C=C6)=N5)=C5C=C4)N3)=C3C=C1.Cl.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 28.1 | 50 | |
| DMSO | 28.1 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 561.93. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.56 mL | 17.8 mL | 35.59 mL |
| 2.5 mM | 0.71 mL | 3.56 mL | 7.12 mL |
| 5 mM | 0.36 mL | 1.78 mL | 3.56 mL |
| 25 mM | 0.07 mL | 0.36 mL | 0.71 mL |
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Product Datasheets
References
References are publications that support the biological activity of the product.
Portugal and Waring (1988) Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochim.Biophys.Acta. 949 158 PMID: 2449244
Loken (1980) Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry 1 136 PMID: 7028425
If you know of a relevant reference for Hoechst 33342, please let us know.
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Keywords: Hoechst 33342, Hoechst 33342 supplier, DNA, stains, fluorescent, dyes, nucleic, acid, cell, permeable, Fluorescent, Stains, 5117, Tocris Bioscience
Citations for Hoechst 33342
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Immunostaining of HEK 293 cells
Works well at 1:2500 dilution incubated for 20 minutes at room temperature just before mounting the coverslips with the mounting media
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