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Submit ReviewHoechst 33342
Description: Fluorescent dye for staining DNA
Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
Purity: ≥98% (HPLC)
Biological Activity for Hoechst 33342
Hoechst 33342 stains and binds minor groove of AT-rich regions. Used to quantify DNA in viable cells. Cell permeable fluorescent DNA dye.
Optical Data for Hoechst 33342
| λabs | 350 nm |
|---|---|
| λem | 461 nm |
| Application | Flow Cytometry |
Technical Data for Hoechst 33342
| M. Wt | 561.93 |
| Formula | C27H28N6O.3HCl |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 875756-97-1 |
| PubChem ID | 16760503 |
| InChI Key | JABNPSKWVNCGMX-UHFFFAOYSA-N |
| Smiles | CN(CC2)CCN2C1=CC(N=C(C4=CC(NC(C6=CC=C(OCC)C=C6)=N5)=C5C=C4)N3)=C3C=C1.Cl.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Hoechst 33342
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 28.1 | 50 | |
| DMSO | 28.1 | 50 |
Preparing Stock Solutions for Hoechst 33342
The following data is based on the product molecular weight 561.93. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.56 mL | 17.8 mL | 35.59 mL |
| 2.5 mM | 0.71 mL | 3.56 mL | 7.12 mL |
| 5 mM | 0.36 mL | 1.78 mL | 3.56 mL |
| 25 mM | 0.07 mL | 0.36 mL | 0.71 mL |
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Product Datasheets for Hoechst 33342
References for Hoechst 33342
References are publications that support the biological activity of the product.
Portugal and Waring (1988) Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochim.Biophys.Acta. 949 158 PMID: 2449244
Loken (1980) Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry 1 136 PMID: 7028425
If you know of a relevant reference for Hoechst 33342, please let us know.
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Keywords: Hoechst 33342, Hoechst 33342 supplier, hoechst, staining, dye, DNA, stains, fluorescent, dyes, nucleic, acid, cell, permeable, Fluorescent, Stains, Cell, Viability, and, Dyes, Counterstains, Flow, Cytometry, 5117, Tocris Bioscience
3 Citations for Hoechst 33342
Citations are publications that use Tocris products. Selected citations for Hoechst 33342 include:
Yang et al (2019) SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Autophagy 1 PMID: 30871407
Durymanov et al (2019) Subcutaneous Inoculation of 3D Pancreatic Cancer Spheroids Results in Development of Reproducible Stroma-Rich Tumors. Transl Oncol 12 180 PMID: 30554606
Tabdanov et al (2018) Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing. Cell Rep 25 328 PMID: 30304674
Do you know of a great paper that uses Hoechst 33342 from Tocris? Please let us know.
Reviews for Hoechst 33342
Average Rating: 5 (Based on 3 Reviews.)
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staining nucleus of live cells.
By
Anonymous on 09/10/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Chondrocytes
I used this dye to stain the nucleus of live cells. After making the stock solution, diluted the dye 1:1000 ratio in the culture media/PBS and added to cells and incubated for 5 mins.
Staining nuclei.
By
Anonymous on 10/27/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: PC3
Add Hoechst dyes at 1 ug/mL.Incubate cells at room temperature for 10 minutes.
Nuclear labeling.
By
Anonymous on 11/01/2017
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK 293
Immunostaining of HEK 293 cells
Works well at 1:2500 dilution incubated for 20 minutes at room temperature just before mounting the coverslips with the mounting media
Literature in this Area
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Fluorescent Probes and Dyes Research Product Guide
This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes
- Fluorescent Dyes
- Dyes for Flow Cytometry
- Fluorescent Probes
- Anti-fade Reagents
- Bioluminescent Substrates
- Fluorogenic Dyes for Light-Up Aptamers
- Fluorescent Probes for Imaging Bacteria
- TSA Reagents for Enhancing IHC, ICC & FISH Signals