Hoechst 33342

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Cat.No. 5117 - Hoechst 33342 | C27H28N6O.3HCl | CAS No. 23491-52-3
Description: Fluorescent dye for labeling DNA
Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Cell permeable fluorescent DNA stain; binds minor groove of AT-rich regions. Used to quantify DNA in viable cells.

Technical Data

M. Wt 561.93
Formula C27H28N6O.3HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 23491-52-3
PubChem ID 16760503
InChI Key JABNPSKWVNCGMX-UHFFFAOYSA-N
Smiles CN(CC2)CCN2C1=CC(N=C(C4=CC(NC(C6=CC=C(OCC)C=C6)=N5)=C5C=C4)N3)=C3C=C1.Cl.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 28.1 50
water 56.19 100

Preparing Stock Solutions

The following data is based on the product molecular weight 561.93. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.78 mL 8.9 mL 17.8 mL
5 mM 0.36 mL 1.78 mL 3.56 mL
10 mM 0.18 mL 0.89 mL 1.78 mL
50 mM 0.04 mL 0.18 mL 0.36 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Portugal and Waring (1988) Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochim.Biophys.Acta. 949 158 PMID: 2449244

Loken (1980) Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry 1 136 PMID: 7028425


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