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Submit ReviewPhalloidin-FITC
Description: Green fluorescent cytoskeleton stain
Chemical Name: 7-[(4R)-N5-[[(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)amino]thioxomethyl]-4-hydroxy-4-methyl-L-ornithine]phalloidin
Purity: ≥90% (HPLC)
Biological Activity for Phalloidin-FITC
Phalloidin-FITC is a green fluorescent cytoskeleton stain. Binds and labels F-actin. For most fluorescent imaging applications, cells should be fixed and permeablilized. Excitation/emission maxima λ ~496/516 nm.
We suggest making up a stock solution of this product by dissolving the vial contents in 1.5 mL methanol or DMSO. Please refer to the protocol for further information on using this product.
Scientific Data
Fluorescence Imaging
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Applications in HeLa Cells for Phalloidin-FITC. Application of Phalloidin-FITC: HeLa cells were labeled with 150 nM Phalloidin-FITC for 20 minutes. Counterstained with DAPI (Catalog # 5748).
Technical Data for Phalloidin-FITC
| M. Wt | 1177.26 |
| Formula | C56H60N10O15S2 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 915026-99-2 |
| PubChem ID | 131676694 |
| InChI Key | CJEWZWZNRFVJSM-PNHWZQMUSA-N |
| Smiles | C[C@H](O)[C@H]1NC(=O)C(C)NC(=O)[C@H](C[C@@](C)(O)CNC(=S)NC2=CC(C(O)=O)=C(C=C2)C2=C3C=CC(=O)C=C3OC3=C2C=CC(O)=C3)NC(=O)[C@@H]2CC3=C(NC4=C3C=CC=C4)SC[C@@H](NC1=O)C(=O)N1C[C@H](O)C[C@H]1C(=O)N[C@@H](C)C(=O)N2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Phalloidin-FITC
References for Phalloidin-FITC
References are publications that support the biological activity of the product.
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Keywords: Phalloidin-FITC, Phalloidin-FITC supplier, green, fluorescent, actin, stain, phalloidin, fluorescein, filament, probe, Organelle, and, Cell, Structure, Stains, Fluorescent, Actin, Probes, 5782, Tocris Bioscience
Citations for Phalloidin-FITC
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Protocols for Phalloidin-FITC
The following protocol features additional information for the use of Phalloidin-FITC (Cat. No. 5782).
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
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