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Submit ReviewPhalloidin-FITC
Description: Green-fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)
Chemical Name: 7-[(4R)-N5-[[(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)amino]thioxomethyl]-4-hydroxy-4-methyl-L-ornithine]phalloidin
Purity: ≥90% (HPLC)
Biological Activity for Phalloidin-FITC
Key information: Phalloidin-FITC is a green-fluorescent cytoskeleton stain. It binds and labels specifically F-actin.
Used for: Phalloidin-FITC can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.
Application: fluorescent microscopy, immunofluorescence (IHC, ICC).
Properties and Photophysical Data: Phalloidin-FITC is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Fluorescein isothiocyanate (FITC; Cat. No. 5440) dye. Phalloidin binds F-actin with high selectivity allowing Phalloidin-FITC to stain actin with high-contrast discrimination. Excitation and emission maxima (λ) are 496 nm and 516 nm, respectively.
We suggest making up a stock solution of this product by dissolving the vial contents in 1.5 mL methanol or DMSO. Please refer to the protocol for further information on using this product.
Scientific Data
Fluorescence Imaging
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Applications in HeLa Cells for Phalloidin-FITC. Application of Phalloidin-FITC: HeLa cells were labeled with 150 nM Phalloidin-FITC for 20 minutes. Counterstained with DAPI (Catalog # 5748).
Optical Data for Phalloidin-FITC
| λabs | 496 nm |
|---|---|
| λem | 516 nm |
| Application | Fluorescent Microscopy, Immunofluorescence (IHC, ICC). |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Phalloidin-FITC
| M. Wt | 1177.26 |
| Formula | C56H60N10O15S2 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 915026-99-2 |
| PubChem ID | 131676694 |
| InChI Key | CJEWZWZNRFVJSM-PNHWZQMUSA-N |
| Smiles | C[C@H](O)[C@H]1NC(=O)C(C)NC(=O)[C@H](C[C@@](C)(O)CNC(=S)NC2=CC(C(O)=O)=C(C=C2)C2=C3C=CC(=O)C=C3OC3=C2C=CC(O)=C3)NC(=O)[C@@H]2CC3=C(NC4=C3C=CC=C4)SC[C@@H](NC1=O)C(=O)N1C[C@H](O)C[C@H]1C(=O)N[C@@H](C)C(=O)N2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Phalloidin-FITC
References for Phalloidin-FITC
References are publications that support the biological activity of the product.
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Keywords: Phalloidin-FITC, Phalloidin-FITC supplier, green, fluorescent, actin, stain, phalloidin, fluorescein, filament, probe, Organelle, and, Cell, Structure, Stains, Fluorescent, Actin, Probes, 5782, Tocris Bioscience
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Protocols for Phalloidin-FITC
The following protocol features additional information for the use of Phalloidin-FITC (Cat. No. 5782).
Literature in this Area
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Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
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