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Submit ReviewNucleoLIVE™ Non-Toxic Dye New
Description: Ready-to-use fluorescent nuclear stain for live cell imaging, cell toxicity, viability and morphology of 2D and 3D cell cultures
Purity: ≥90% (HPLC)
Biological Activity for NucleoLIVE™ Non-Toxic Dye
NucleoLIVE™ Non-Toxic Dye is a ready-to-use fluorescent nuclear stain for live cell imaging. NucleoLIVE™ Non-Toxic Dye is highly suited for long term 2D and 3D cell organoids imaging. Able to multiplex with Live-dead stain for multiplexed phenotypic imaging. NucleoLIVE™ Non-Toxic Dye is suitable for monitoring precise tracking of cell proliferation, differentiation, and responses to stimuli over time. Allow wash-free imaging. NucleoLIVE™ Non-Toxic Dye is provided as 50 µL of 1000x concentrate in DMSO. Excitation and emission maxima (λ) are 568 nM and 590 nM, respectively.
Please refer to this protocol for further information on how to use this product.
NucleoLIVE™ is a trademark of Saguaro Biosciences.
Scientific Data
Live-cell Imaging
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NucleoLIVE™ Non-Toxic Dye Application. NucleoLIVE™ staining of 3D brain organoids under hypoxic conditions. Live imaging shows deep penetration and uniform nuclear staining (1:500, red), demonstrating suitability for complex 3D models.
Live-cell Imaging
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NucleoLIVE™ Non-Toxic Dye Application. MCF-7 cells co-stained with NucleoLIVE™ (1:500) and Hoechst 33342 following 4-hour incubation. Image captured at 40× magnification, showing nuclear co-localization and live-cell compatibility.
NucleoLIVE™ Non-Toxic Dye Application. Comparison of proliferation rates in MCF-7 cells: stable nuclear GFP reporter line versus NucleoLIVE™ staining at various concentrations (1×: 1:1,000; 0.5×: 1:2,000; 0.25×: 1:4,000). Controls include untreated (“Medium”) and 0.1% DMSO. Assays performed on an Incucyte Live-Cell Analysis System by Prof. John Hawse, Mayo Clinic.
Licensing Information
Sold under license from Saguaro Biosciences
Optical Data for NucleoLIVE™ Non-Toxic Dye
| λabs | 568 nm |
|---|---|
| λem | 590 nm |
| Cell Permeable | Yes |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for NucleoLIVE™ Non-Toxic Dye
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for NucleoLIVE™ Non-Toxic Dye
| Solubility | Soluble in DMSO (supplied pre-dissolved in DMSO, 1000x) |
Product Datasheets for NucleoLIVE™ Non-Toxic Dye
References for NucleoLIVE™ Non-Toxic Dye
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