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Description: Fluorescent lipid peroxide probe for live cell imaging
Chemical Name: 2-[4-(Diphenylphosphino)phenyl]-9-(3,6,9,12-tetraoxatridec-1-yl)anthra[2,1,9-def :6,5,10-d'e'f']diisoquinoline-1,3,8,10(2H,9H)-tetrone
Purity: ≥90% (HPLC)
Biological Activity for Liperfluo
Key information: Liperfluo is a fluorescent probe for the selective detection and imaging of lipid peroxides in living cells. Upon reaction with lipid peroxides, Liperfluo is oxidized and becomes fluorescent.
Used for: imaging lipid peroxides in living cells. Liperfluo can be used to monitor lipid peroxidation in ferroptosis research.
Application: fluorescent microscopy and flow cytometry.
Properties and Photophysical Data: Liperfluo has a high solubility in organic solvents. It displays minimal photo damage and auto-fluorescence and shows high selectivity to lipid peroxidases over ROS and other radical species. Excitation and emission maxima (λ) measured in ethanol are 524 nm and 535 nm, respectively; quantum yield (oxidized form) = 0.85.
Scientific Data
Confocal Microscopy
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Fluorescence images of lipid peroxidation in HeLa cells. HeLa cells were pre-incubated in serum-free medium containing 1 μM Liperfluo at 37 °C for 30 min. in a 5% CO2 incubator. The medium was replaced by medium containing serum and 500 μM t-BHP and incubated at 37 °C for 60 min. in a 5% CO2 incubator. The cells were observed under a confocal fluorescence microscope.
Optical Data for Liperfluo
| Emission Color | Green |
|---|---|
| λabs | 524 nm |
| λem | 535 nm |
| Quantum Yield (φ) | 0.85 |
| Cell Permeable | Yes |
| Application | fluorescent microscopy, flow cytometry |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Liperfluo
| M. Wt | 840.87 |
| Formula | C51H41N2O8P |
| Storage | Store at +4°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 1448846-35-2 |
| PubChem ID | 166642656 |
| InChI Key | MDGOURRQWXHVBZ-UHFFFAOYSA-N |
| Smiles | O=C1C2=CC=C3C4=CC=C5C(N(CCOCCOCCOCCOC)C(C6=CC=C(C4=C56)C7=C3C2=C(C(N1C8=CC=C(P(C9=CC=CC=C9)C%10=CC=CC=C%10)C=C8)=O)C=C7)=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Liperfluo
References for Liperfluo
References are publications that support the biological activity of the product.
Yamanaka et al (2012) A novel fluorescent probe with high sensitivity and selective detection of lipid hydroperoxides in cells. RSC Adv. 2 7894
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
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