MitoBrilliant™ 646

Pricing Availability   Qty
Description: Universal red fluorescent mitochondrial stain for both live and fixed cells
Purity: ≥95% (HPLC)
Protocols (1)
Literature (2)

Biological Activity for MitoBrilliant™ 646

MitoBrilliant probes have been developed to overcome some common limitations encountered with standard mitochondrial trackers, offering clearer answers to scientific questions. They are next-generation fluorescent stains for the localization and tracking of mitochondria in both live and fixed cells. The range harnesses Janelia Fluor® dye technology, conferring some of the properties that make these widely used dyes, into mitochondrial stains.

MitoBrilliant™ 646 is a next-generation universal stain for mitochondria in both live and fixed cells. It is retained in mitochondria following fixation with exceptionally clear staining and is retained in the mitochondria of live cells following loss of the mitochondrial membrane potential. Additionally, MitoBrilliant™ 646 can be used to stain pre-fixed cells/tissue, making this dye suitable for IHC/ICC.

Two dyes that accumulate in the mitochondria of live cells in a mitochondrial membrane potential (Δψm) dependent manner are also available: MitoBrilliant™ Live 646 (red emission) and MitoBrilliant™ Live 549 (yellow/orange emission).

Please refer to the protocol for guidelines on product use, and download the MitoBrilliant Product Guide to view data for each of the dyes in different applications.

MitoBrilliant is a trademark of Bio-Techne Corp.

Scientific Data

Live-cell image of mitochondria stained with MitoBrilliant™ 646 HeLa cells were incubated with MitoBrilliant™ 646 (100 nM) for 40 minutes and counterstained with DAPI (Catalog # 5748). Image was taken using an LSM880 Confocal using a 63x objective. Scale bar = 10 µm.

Product Name Core dye structure Abs/Em (nm) Δψm dependent Live/Fixed cell use Stain pre-fixed cells? Image without wash step Demonstrated Applications
MitoBrilliant™ 646
Cat. No. 7700
Janelia Fluor® technology 655/668 No* Suitable for both live and fixed cell work Yes Yes, but replacing media recommended Fixed-cell imaging,
Live-cell imaging,
Flow Cytometry,
Super-resolution microscopy – STED,
High-content screening
MitoBrilliant™ Live 646
Cat. No. 7417
Janelia Fluor® technology 648/662 Yes Live-cell work only No Yes, but replacing media recommended Live-cell imaging,
Flow Cytometry,
High-content screening
MitoBrilliant™ Live 549
Cat. No. 7693
Janelia Fluor® technology 550/568 Yes Live-cell work only No Yes, but replacing media recommended Live-cell imaging,
Flow Cytometry,
High-content screening

Optical Data for MitoBrilliant™ 646

MitoBrilliant™ 646 Dye Spectra
Emission Color Red
λabs 655 nm
λem 668 nm
Extinction Coefficient (ε) 125000 M-1cm-1
Closest Laser line 640 nm
Application Fixed-cell imaging, Live-cell imaging, Flow Cytometry, IHC/ICC, Super-resolution microscopy – STED,

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for MitoBrilliant™ 646

M. Wt 493.55
Storage Store at -20°C
Purity ≥95% (HPLC)

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for MitoBrilliant™ 646

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 4.94 10

Preparing Stock Solutions for MitoBrilliant™ 646

The following data is based on the product molecular weight 493.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 20.26 mL 101.31 mL 202.61 mL
0.5 mM 4.05 mL 20.26 mL 40.52 mL
1 mM 2.03 mL 10.13 mL 20.26 mL
5 mM 0.41 mL 2.03 mL 4.05 mL

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Product Datasheets for MitoBrilliant™ 646

References for MitoBrilliant™ 646

References are publications that support the biological activity of the product.

If you know of a relevant reference for MitoBrilliant™ 646, please let us know.

Keywords: MitoBrilliant 646, MitoBrilliant 646 supplier, MitoBrilliant, red, fluorescent, mitochondrial, marker, stain, mitotracker, mitochondria, Flow, Cytometry, Mitobrilliant, 7700, Tocris Bioscience

Citations for MitoBrilliant™ 646

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Protocols for MitoBrilliant™ 646

The following protocol features additional information for the use of MitoBrilliant™ 646 (Cat. No. 7700).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

MitoBrilliant Research Product Guide

MitoBrilliant Research Product Guide

This product guide provides a background, protocols and data from use in different research applications for our Mitobrilliant™ fluorescent mitochondrial probes:

  • MitoBrilliant™ 646
  • MitoBrilliant™ Live 646
  • MitoBrilliant™ Live 549
Novel Live-cell and Fixable Mitochondrial Probes Poster

Novel Live-cell and Fixable Mitochondrial Probes Poster

Fluorescent probes that accumulate in the mitochondria are useful tools to explore mitochondrial function and cell health. This poster reports the development inhouse and characterization of novel fluorescent probes, known as MitoBrilliant that specifically localize to mitochondria due to mitochondrial membrane potential. The probes are suitable for live or fixed cell staining. The data demonstrate the specificity and enhanced properties of our MitoBrilliant dyes and their use in a range of applications. This poster was presented at CYTO 2022, June 2022, Philadelphia, USA.