MitoBrilliant™ Live 646

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Description: Red fluorescent mitochondrial stain for live cells, Δψm dependent
Purity: ≥95% (HPLC)
Protocols (1)
Literature (2)

Biological Activity for MitoBrilliant™ Live 646

MitoBrilliant probes have been developed to overcome some common limitations encountered with standard mitochondrial trackers, offering clearer answers to scientific questions. They are next-generation fluorescent stains for the localization and tracking of mitochondria in both live and fixed cells. The range harnesses Janelia Fluor® dye technology, conferring some of the properties that make these widely used dyes, into mitochondrial stains.

The MitoBrilliant™ Live dyes accumulate in the mitochondria of live cells in a mitochondrial membrane potential (Δψm) dependent manner. Upon loss of the mitochondrial membrane potential, the dyes disperse, and so provide a dynamic assessment of the mitochondrial membrane potential in live cells. Two dyes are available: MitoBrilliant™ Live 646 (red emission) and MitoBrilliant™ Live 549 (yellow/orange emission).

A universal stain for mitochondria in both live and fixed cells is also available: MitoBrilliant™ 646.

Please refer to the protocol for guidelines on product use, and download the MitoBrilliant Product Guide to view data for each of the dyes in different applications.

MitoBrilliant is a trademark of Bio-Techne Corp.

Scientific Data

Live-cell image of mitochondria stained with MitoBrilliant™ Live 646 HeLa cells were incubated with MitoBrilliant™ Live 646 (100 nM) for 40 minutes and counter stained with DAPI (Catalog # 5748). Image was taken using an LSM880 Confocal using a 63x objective. Scale bar = 10 µm.

Product Name Core dye structure Abs/Em (nm) Δψm dependent Live/Fixed cell use Stain pre-fixed cells? Image without wash step Demonstrated Applications
MitoBrilliant™ 646
Cat. No. 7700
Janelia Fluor® technology 655/668 No* Suitable for both live and fixed cell work Yes Yes, but replacing media recommended Fixed-cell imaging,
Live-cell imaging,
Flow Cytometry,
Super-resolution microscopy – STED,
High-content screening
MitoBrilliant™ Live 646
Cat. No. 7417
Janelia Fluor® technology 648/662 Yes Live-cell work only No Yes, but replacing media recommended Live-cell imaging,
Flow Cytometry,
High-content screening
MitoBrilliant™ Live 549
Cat. No. 7693
Janelia Fluor® technology 550/568 Yes Live-cell work only No Yes, but replacing media recommended Live-cell imaging,
Flow Cytometry,
High-content screening

Optical Data for MitoBrilliant™ Live 646

MitoBrilliant™ Live 646 Dye Spectra
Emission Color Red
λabs 648 nm
λem 662 nm
Extinction Coefficient (ε) 127800 M-1cm-1
Closest Laser line 640 nm
Application Live-cell Imaging, Flow Cytometry, High-content Screening

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for MitoBrilliant™ Live 646

M. Wt 445.08
Storage Store at -20°C
Purity ≥95% (HPLC)

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for MitoBrilliant™ Live 646

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 8.9 20

Preparing Stock Solutions for MitoBrilliant™ Live 646

The following data is based on the product molecular weight 445.08. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 11.23 mL 56.17 mL 112.34 mL
1 mM 2.25 mL 11.23 mL 22.47 mL
2 mM 1.12 mL 5.62 mL 11.23 mL
10 mM 0.22 mL 1.12 mL 2.25 mL

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Product Datasheets for MitoBrilliant™ Live 646

References for MitoBrilliant™ Live 646

References are publications that support the biological activity of the product.

If you know of a relevant reference for MitoBrilliant™ Live 646, please let us know.

Keywords: MitoBrilliant Live 646, MitoBrilliant Live 646 supplier, MitoBrilliant, red, fluorescent, mitochondrial, marker, stain, mitotracker, mitochondria, live, cell, imaging, Flow, Cytometry, Mitobrilliant, 7417, Tocris Bioscience

Citations for MitoBrilliant™ Live 646

Citations are publications that use Tocris products.

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Protocols for MitoBrilliant™ Live 646

The following protocol features additional information for the use of MitoBrilliant™ Live 646 (Cat. No. 7417).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

MitoBrilliant Research Product Guide

MitoBrilliant Research Product Guide

This product guide provides a background, protocols and data from use in different research applications for our Mitobrilliant™ fluorescent mitochondrial probes:

  • MitoBrilliant™ 646
  • MitoBrilliant™ Live 646
  • MitoBrilliant™ Live 549
Novel Live-cell and Fixable Mitochondrial Probes Poster

Novel Live-cell and Fixable Mitochondrial Probes Poster

Fluorescent probes that accumulate in the mitochondria are useful tools to explore mitochondrial function and cell health. This poster reports the development inhouse and characterization of novel fluorescent probes, known as MitoBrilliant that specifically localize to mitochondria due to mitochondrial membrane potential. The probes are suitable for live or fixed cell staining. The data demonstrate the specificity and enhanced properties of our MitoBrilliant dyes and their use in a range of applications. This poster was presented at CYTO 2022, June 2022, Philadelphia, USA.