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MitoBrilliant probes have been developed to overcome some common limitations encountered with standard mitochondrial trackers, offering clearer answers to scientific questions. They are next-generation fluorescent stains for the localization and tracking of mitochondria in both live and fixed cells. The range harnesses Janelia Fluor® dye technology, conferring some of the properties that make these widely used dyes, into mitochondrial stains.
The MitoBrilliant™ Live dyes accumulate in the mitochondria of live cells in a mitochondrial membrane potential (Δψm) dependent manner. Upon loss of the mitochondrial membrane potential, the dyes disperse, and so provide a dynamic assessment of the mitochondrial membrane potential in live cells. Two dyes are available: MitoBrilliant™ Live 646 (red emission) and MitoBrilliant™ Live 549 (yellow/orange emission).
A universal stain for mitochondria in both live and fixed cells is also available: MitoBrilliant™ 646.
Please refer to the protocol for guidelines on product use, and download the MitoBrilliant Product Guide to view data for each of the dyes in different applications.
MitoBrilliant is a trademark of Bio-Techne Corp.
Live-cell image of mitochondria stained with MitoBrilliant™ Live 646 HeLa cells were incubated with MitoBrilliant™ Live 646 (100 nM) for 40 minutes and counter stained with DAPI (Catalog # 5748). Image was taken using an LSM880 Confocal using a 63x objective. Scale bar = 10 µm.
|Product Name||Core dye structure||Abs/Em (nm)||Δψm dependent||Live/Fixed cell use||Stain pre-fixed cells?||Image without wash step||Demonstrated Applications|| MitoBrilliant™ 646 |
Cat. No. 7700
|Janelia Fluor® technology||655/668||No*||Suitable for both live and fixed cell work||Yes||Yes, but replacing media recommended|| Fixed-cell imaging, |
Super-resolution microscopy – STED,
| MitoBrilliant™ Live 646 |
Cat. No. 7417
|Janelia Fluor® technology||648/662||Yes||Live-cell work only||No||Yes, but replacing media recommended|| Live-cell imaging, |
| MitoBrilliant™ Live 549 |
Cat. No. 7693
|Janelia Fluor® technology||550/568||Yes||Live-cell work only||No||Yes, but replacing media recommended|| Live-cell imaging, |
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 445.08. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.2 mM||11.23 mL||56.17 mL||112.34 mL|
|1 mM||2.25 mL||11.23 mL||22.47 mL|
|2 mM||1.12 mL||5.62 mL||11.23 mL|
|10 mM||0.22 mL||1.12 mL||2.25 mL|
References are publications that support the biological activity of the product.
If you know of a relevant reference for MitoBrilliant™ Live 646, please let us know.
Keywords: MitoBrilliant Live 646, MitoBrilliant Live 646 supplier, MitoBrilliant, red, fluorescent, mitochondrial, marker, stain, mitotracker, mitochondria, live, cell, imaging, Flow, Cytometry, Mitobrilliant, 7417, Tocris Bioscience
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The following protocol features additional information for the use of MitoBrilliant™ Live 646 (Cat. No. 7417).
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.