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This is intended as a guide only; for full experimental details please read the MitoBrilliant Product Guide provided.
Next-generation fluorescent stains for the localization and tracking of mitochondria
|Product||Unit Size||Abs/Em (nm)||Extinction Coefficient (M-1 cm-1)||Δψm Dependent?||Stain pre-fixed cells?|
|MitoBrilliant™ 646 Cat. No. 7700||5x50 µg||655/668||125,000||No*||Yes|
|MitoBrilliant™ Live 646 Cat. No. 7417||5x50 µg||648/662||127,800||Yes||No|
|MitoBrilliant™ Live 549 Cat. No. 7693||5x50 µg||550/568||100,000||Yes||No|
* In live-cell staining, the mitochondrial membrane potential (Δψm) helps to drive initial recruitment of the dye into mitochondria. After staining, localization of the dye becomes insensitive to Δψm changes. In pre-fixed cell staining, MitoBrilliant™ 646 can localize and be retained in mitochondria without Δψm. For more information on MitoBrilliant™ including application data, please refer to the MitoBrilliant Product Guide.
Storage and Handling
Store at -20°C and protect from light. We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials at -20°C or below and used within 1 month.
Reconstitution and Stock Solution Preparation
Prepare a 1mM stock solution by adding high quality, anhydrous DMSO to one vial of MitoBrilliant™ dye. For more information on preparing stock solutions and reconstitution, please see our Molarity Calculator and Reconstitution Calculator.
|Product||Molecular Weight||Quantity per vial||Recommended reconstitution|
|MitoBrilliant™ 646 Cat. No. 7700||493.6||50 µg (101 nmoles)||Add 101 µL DMSO for a 1 mM stock solution|
|MitoBrilliant™ Live 646 Cat. No. 7417||445.1||50 µg (112 nmoles)||Add 112 µL DMSO for a 1 mM stock solution|
|MitoBrilliant™ Live 549 Cat. No. 7693||417.0||50 µg (120 nmoles)||Add 120 µL DMSO for a 1 mM stock solution|
Staining Live Cells
Staining Cells in Suspension for Flow Cytometry
Staining Fixed Cells - Only Applicable to: MitoBrilliant™ 646 (Cat. No. 7700)
MitoBrilliant™ 646 can be applied at either the pre-fixation or post-fixation stages of sample preparation. For more application information, please refer to the MitoBrilliant Product Guide.