This is intended as a guide only; for full experimental details please read the MitoBrilliant Product Guide provided.
Next-generation fluorescent stains for the localization and tracking of mitochondria
Product | Unit Size | Abs/Em (nm) | Extinction Coefficient (M-1 cm-1) | Δψm Dependent? | Stain pre-fixed cells? |
---|---|---|---|---|---|
MitoBrilliant™ 646 Cat. No. 7700 | 5x50 µg | 655/668 | 125,000 | No* | Yes |
MitoBrilliant™ Live 646 Cat. No. 7417 | 5x50 µg | 648/662 | 127,800 | Yes | No |
MitoBrilliant™ Live 549 Cat. No. 7693 | 5x50 µg | 550/568 | 100,000 | Yes | No |
* In live-cell staining, the mitochondrial membrane potential (Δψm) helps to drive initial recruitment of the dye into mitochondria. After staining, localization of the dye becomes insensitive to Δψm changes. In pre-fixed cell staining, MitoBrilliant™ 646 can localize and be retained in mitochondria without Δψm. For more information on MitoBrilliant™ including application data, please refer to the MitoBrilliant Product Guide.
Storage and Handling
Store at -20°C and protect from light. We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials at -20°C or below and used within 1 month.
Reconstitution and Stock Solution Preparation
Prepare a 1mM stock solution by adding high quality, anhydrous DMSO to one vial of MitoBrilliant™ dye. For more information on preparing stock solutions and reconstitution, please see our Molarity Calculator and Reconstitution Calculator.
Product | Molecular Weight | Quantity per vial | Recommended reconstitution |
---|---|---|---|
MitoBrilliant™ 646 Cat. No. 7700 | 493.6 | 50 µg (101 nmoles) | Add 101 µL DMSO for a 1 mM stock solution |
MitoBrilliant™ Live 646 Cat. No. 7417 | 445.1 | 50 µg (112 nmoles) | Add 112 µL DMSO for a 1 mM stock solution |
MitoBrilliant™ Live 549 Cat. No. 7693 | 417.0 | 50 µg (120 nmoles) | Add 120 µL DMSO for a 1 mM stock solution |
Staining Live Cells
Staining Cells in Suspension for Flow Cytometry
Staining Fixed Cells - Only Applicable to: MitoBrilliant™ 646 (Cat. No. 7700)
MitoBrilliant™ 646 can be applied at either the pre-fixation or post-fixation stages of sample preparation. For more application information, please refer to the MitoBrilliant Product Guide.