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Fluorescent ligand that binds to the α4β1 integrin (VLA-4) with high affinity (Kd values are 0.3 nM and 12 nM for binding to U937 cells in the presence and absence of Mn2+ respectively). Used to detect VLA-4 affinity and conformational changes.
|Sequence||LDVPAAK (Modifications: Leu-1 N-2-[4-[[[(2-methylphenyl)amino]carbonyl]amino]phenyl]acetyl-, Lys-6 N-FITC conjugate)|
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solubility||Soluble to 1 mg/ml in PBS (pH 7.4)|
References are publications that support the biological activity of the product.
Chigaev et al (2001) Real time analysis of the affinity regulation of alpha 4-integrin. The physiologically activated receptor is intermediate in affinity between resting and Mn2+ or antibody activation. J.Biol.Chem. 276 48670 PMID: 11641394
Njus et al (2009) Conformational mAb as a tool for integrin ligand discovery. Assay Drug Dev.Technol. 7 507 PMID: 19754304
Chigaev et al (2003) FRET detection of cellular α4-integrin conformational activation. Biophys.J. 85 3951 PMID: 14645084
If you know of a relevant reference for LDV FITC, please let us know.
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