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Biological Activity for Kyoto Probe-1
Kyoto Probe-1 distinguishes between undifferentiated iPS/ES cells and differentiated cells, so may be used for monitoring pluripotency during hESC or iPSC maintenance. Selectivity of labeling is due to ATP-binding cassette transporters ABCB1 and ABCG2 - these mediate efflux of Kyoto Probe-1 and are expressed in differentiated cells, but are repressed in hiPSC and hESC. Kyoto Probe-1 distinguishes between SSEA-4-positive hESCs and human early hematopoietic cells expressing CD45, CD235, CD41a, or CD43 in flow cytometry experiments, making it a valuable tool for studying early hematopoiesis. The product also distinguishes between hiPSCs and hiPSC-derived cardiomyocytes. Note that Kyoto Probe-1 does not differentiate between hiPSCs and differentiated human neuronal stem cells.
Kyoto Probe-1 localizes to the mitochondria in undifferentiated iPS/ES cells only. Suitable in flow cytometry and live cell imaging.
Excitation maximum = 515 nm; emission maximum = 529 nm; Quantum yield = 0.45
Sold under license from New York University
Optical Data for Kyoto Probe-1
Technical Data for Kyoto Probe-1
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Kyoto Probe-1
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Kyoto Probe-1
The following data is based on the product molecular weight 418.35. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.39 mL||11.95 mL||23.9 mL|
|5 mM||0.48 mL||2.39 mL||4.78 mL|
|10 mM||0.24 mL||1.2 mL||2.39 mL|
|50 mM||0.05 mL||0.24 mL||0.48 mL|
References for Kyoto Probe-1
References are publications that support the biological activity of the product.
Mao et al (2017) A synthetic hybrid molecule for the selective removal of human pluripotent stem cells from cell mixtures. Angew.Chem.Int.Ed.Engl. 56 1765 PMID: 28067441
Hirata et al (2014) A chemical probe that labels human pluripotent stem cells. Cell Rep. 6 1165 PMID: 24613351
Miyagi-Shiohira et al (2020) Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells. Sci.Rep. 10 18084 PMID: 33093580
If you know of a relevant reference for Kyoto Probe-1, please let us know.
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Citations for Kyoto Probe-1
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Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.