Kyoto Probe-1

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Cat.No. 7419 - Kyoto Probe-1 | C19H13FN2O.CF3CO2H | CAS No. 2088021-81-0
Description: Fluorescent probe that selectively identifies undifferentiated iPS/ES cells
Alternative Names: KP1
Chemical Name: 9-(4-Fluorophenyl)-3-imino-3H-xanthen-6-amine trifluoroacetate
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (5)

Biological Activity for Kyoto Probe-1

Kyoto Probe-1 or KP-1 distinguishes between undifferentiated iPS/ES cells and differentiated cells, so may be used for monitoring pluripotency during hESC or iPSC maintenance. Kyoto Probe 1 distinguishes between SSEA-4-positive hESCs and human early hematopoietic cells expressing CD45, CD235, CD41a, or CD43 in flow cytometry experiments, making it a valuable tool for studying early hematopoiesis. The product also distinguishes between hiPSCs and hiPSC-derived cardiomyocytes. Note that Kyoto Probe 1 does not differentiate between hiPSCs and differentiated human neuronal stem cells.

Kyoto Probe 1 localizes to the mitochondria in undifferentiated iPS/ES cells only. Suitable in flow cytometry and live cell imaging.

Excitation maximum = 515 nm; emission maximum = 529 nm; Quantum yield = 0.45

Licensing Information

Sold under license from New York University

Optical Data for Kyoto Probe-1

Kyoto Probe-1 Dye Spectra
Emission Color Yellow
λabs 515 nm
λem 529 nm
Quantum Yield (φ) 0.45
Cell Permeable Yes

Technical Data for Kyoto Probe-1

M. Wt 418.35
Formula C19H13FN2O.CF3CO2H
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 2088021-81-0
InChI Key ADFLZJALCLDWDM-UHFFFAOYSA-N
Smiles [O-]C(C(F)(F)F)=O.[NH2+]=C1C=C2OC3=C(C(C4=CC=C(C=C4)F)=C2C=C1)C=CC(N)=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Kyoto Probe-1

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 41.83 100

Preparing Stock Solutions for Kyoto Probe-1

The following data is based on the product molecular weight 418.35. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.39 mL 11.95 mL 23.9 mL
5 mM 0.48 mL 2.39 mL 4.78 mL
10 mM 0.24 mL 1.2 mL 2.39 mL
50 mM 0.05 mL 0.24 mL 0.48 mL

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Product Datasheets for Kyoto Probe-1

References for Kyoto Probe-1

References are publications that support the biological activity of the product.

Ahn et al (2007) Combinatorial rosamine library and application to in vivo glutathione probe. J.Am.Chem.Soc. 129 4510 PMID: 17378562

Mao et al (2017) A synthetic hybrid molecule for the selective removal of human pluripotent stem cells from cell mixtures. Angew.Chem.Int.Ed.Engl. 56 1765 PMID: 28067441

Hirata et al (2014) A chemical probe that labels human pluripotent stem cells. Cell Rep. 6 1165 PMID: 24613351


If you know of a relevant reference for Kyoto Probe-1, please let us know.

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Citations for Kyoto Probe-1

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