Kyoto Probe-1

Pricing Availability   Qty
Description: Fluorescent probe that selectively identifies undifferentiated iPS/ES cells
Alternative Names: KP1
Chemical Name: 9-(4-Fluorophenyl)-3-imino-3H-xanthen-6-amine trifluoroacetate
Purity: ≥95% (HPLC)
Literature (4)

Biological Activity for Kyoto Probe-1

Kyoto Probe-1 distinguishes between undifferentiated iPS/ES cells and differentiated cells, so may be used for monitoring pluripotency during hESC or iPSC maintenance. Selectivity of labeling is due to ATP-binding cassette transporters ABCB1 and ABCG2 - these mediate efflux of Kyoto Probe-1 and are expressed in differentiated cells, but are repressed in hiPSC and hESC. Kyoto Probe-1 distinguishes between SSEA-4-positive hESCs and human early hematopoietic cells expressing CD45, CD235, CD41a, or CD43 in flow cytometry experiments, making it a valuable tool for studying early hematopoiesis. The product also distinguishes between hiPSCs and hiPSC-derived cardiomyocytes. Note that Kyoto Probe-1 does not differentiate between hiPSCs and differentiated human neuronal stem cells.

Kyoto Probe-1 localizes to the mitochondria in undifferentiated iPS/ES cells only. Suitable in flow cytometry and live cell imaging.

Excitation maximum = 515 nm; emission maximum = 529 nm; Quantum yield = 0.45

Licensing Information

Sold under license from New York University

Optical Data for Kyoto Probe-1

Kyoto Probe-1 Dye Spectra
Emission Color Green
λabs 515 nm
λem 529 nm
Quantum Yield (φ) 0.45
Cell Permeable Yes
Application Flow Cytometry, Live-cell Imaging

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Kyoto Probe-1

M. Wt 418.35
Formula C19H13FN2O.CF3CO2H
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 2088021-81-0
Smiles [O-]C(C(F)(F)F)=O.[NH2+]=C1C=C2OC3=C(C(C4=CC=C(C=C4)F)=C2C=C1)C=CC(N)=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Kyoto Probe-1

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 41.83 100

Preparing Stock Solutions for Kyoto Probe-1

The following data is based on the product molecular weight 418.35. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.39 mL 11.95 mL 23.9 mL
5 mM 0.48 mL 2.39 mL 4.78 mL
10 mM 0.24 mL 1.2 mL 2.39 mL
50 mM 0.05 mL 0.24 mL 0.48 mL

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Product Datasheets for Kyoto Probe-1

References for Kyoto Probe-1

References are publications that support the biological activity of the product.

Mao et al (2017) A synthetic hybrid molecule for the selective removal of human pluripotent stem cells from cell mixtures. Angew.Chem.Int.Ed.Engl. 56 1765 PMID: 28067441

Hirata et al (2014) A chemical probe that labels human pluripotent stem cells. Cell Rep. 6 1165 PMID: 24613351

Miyagi-Shiohira et al (2020) Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells. Sci.Rep. 10 18084 PMID: 33093580

If you know of a relevant reference for Kyoto Probe-1, please let us know.

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