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Description: Potent inhibitor of Wnt/β-catenin signaling
Chemical Name: N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-3-(2-methoxyphenyl)-4-oxothieno[3,2-d]pyrimidin-2-yl)thio]-acetamide
Purity: ≥98% (HPLC)
Citations (2)
Literature (1)
Pathways (1)

Biological Activity for IWP 4

IWP 4 is a potent inhibitor of Wnt/β-catenin signaling (IC50 = 25 nM). Has minimal effect on Notch and Hedgehog signaling pathways. Induces differentiation of cardiomyocytes from human ESCs and iPSCs.

Technical Data for IWP 4

M. Wt 496.62
Formula C23H20N4O3S3
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 686772-17-8
PubChem ID 2155264
Smiles O=C1C3=C(CCS3)N=C(SCC(NC4=NC(C=CC(C)=C5)=C5S4)=O)N1C2=C(OC)C=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for IWP 4

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 0.5 1

Preparing Stock Solutions for IWP 4

The following data is based on the product molecular weight 496.62. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.01 mM 201.36 mL 1006.81 mL 2013.61 mL
0.05 mM 40.27 mL 201.36 mL 402.72 mL
0.1 mM 20.14 mL 100.68 mL 201.36 mL
0.5 mM 4.03 mL 20.14 mL 40.27 mL

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Product Datasheets for IWP 4

Certificate of Analysis / Product Datasheet
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References for IWP 4

References are publications that support the biological activity of the product.

Lian et al (2012) Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc.Natl.Acad.Sci.U.S.A. 109 E1848-57 PMID: 22645348

Chen et al (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat.Chem.Biol. 5 100 PMID: 19125156

Narytnyk et al (2014) Differentiation of human epidermal neural crest stem cells (hEPI-NCSC) into virtually homogenous populations of DArgic neurons. Stem Cell Rev. 10 316 PMID: 24399192

Hoang et al (2018) Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells. Nat Protoc. 13 723 PMID: 29543795

Noor et al (2019) 3D Printing of Personalized Thick and Perfusable Cardiac Patches and Hearts. Adv Sci (Weinh) 6 1900344 PMID: 31179230

If you know of a relevant reference for IWP 4, please let us know.

Keywords: IWP 4, IWP 4 supplier, IWP4, potent, inhibitors, inhibits, Wnt, beta-catenin, β-catenin, b-catenin, cardiomyocytes, ESCs, iPSCs, stem, cells, embryonic, pluripotent, differentiation, Beta-catenin, Cardiomyocyte, Stem, Cells, and, iPSC, Organoids, 5214, Tocris Bioscience

2 Citations for IWP 4

Citations are publications that use Tocris products. Selected citations for IWP 4 include:

Sewanan et al (2019) Extracellular Matrix From Hypertrophic Myocardium Provokes Impaired Twitch Dynamics in Healthy Cardiomyocytes. JACC Basic Transl Sci 4 495 PMID: 31468004

Boroujeni et al (2019) Neural crest stem cells from human epidermis of aged donors maintain their multipotency in vitro and in vivo. Sci Rep 9 9750 PMID: 31278326

Do you know of a great paper that uses IWP 4 from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cancer Research Product Guide

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Pathways for IWP 4

Wnt Signaling Pathway

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.