IWP 4

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Cat.No. 5214 - IWP 4 | C23H20N4O3S3 | CAS No. 686772-17-8
Description: Potent inhibitor of Wnt/β-catenin signaling
Chemical Name: N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-3-(2-methoxyphenyl)-4-oxothieno[3,2-d]pyrimidin-2-yl)thio]-acetamide
Purity: ≥98% (HPLC)
Datasheet
Citations (2)
Reviews (1)
Literature (1)
Pathways (1)

Biological Activity

Potent inhibitor of Wnt/β-catenin signaling (IC50 = 25 nM). Has minimal effect on Notch and Hedgehog signaling pathways. Induces differentiation of cardiomyocytes from human ESCs and iPSCs.

Technical Data

M. Wt 496.62
Formula C23H20N4O3S3
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 686772-17-8
PubChem ID 2155264
InChI Key RHUJMHOIQBDFQR-UHFFFAOYSA-N
Smiles O=C1C3=C(CCS3)N=C(SCC(NC4=NC(C=CC(C)=C5)=C5S4)=O)N1C2=C(OC)C=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 0.5 1

Preparing Stock Solutions

The following data is based on the product molecular weight 496.62. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.01 mM 201.36 mL 1006.81 mL 2013.61 mL
0.05 mM 40.27 mL 201.36 mL 402.72 mL
0.1 mM 20.14 mL 100.68 mL 201.36 mL
0.5 mM 4.03 mL 20.14 mL 40.27 mL

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References

References are publications that support the biological activity of the product.

Lian et al (2012) Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc.Natl.Acad.Sci.U.S.A. 109 E1848-57 PMID: 22645348

Chen et al (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat.Chem.Biol. 5 100 PMID: 19125156

Narytnyk et al (2014) Differentiation of human epidermal neural crest stem cells (hEPI-NCSC) into virtually homogenous populations of DArgic neurons. Stem Cell Rev. 10 316 PMID: 24399192

Hoang et al (2018) Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells. Nat Protoc. 13 723 PMID: 29543795

Noor et al (2019) 3D Printing of Personalized Thick and Perfusable Cardiac Patches and Hearts. Adv Sci (Weinh) 6 1900344 PMID: 31179230


If you know of a relevant reference for IWP 4, please let us know.

Keywords: IWP 4, IWP 4 supplier, IWP4, potent, inhibitors, inhibits, Wnt, beta-catenin, β-catenin, b-catenin, cardiomyocytes, ESCs, iPSCs, stem, cells, embryonic, pluripotent, differentiation, Beta-catenin, Cardiomyocyte, Stem, Cells, and, iPSC, Organoids, 5214, Tocris Bioscience

2 Citations for IWP 4

Citations are publications that use Tocris products. Selected citations for IWP 4 include:

Boroujeni et al (2019) Neural crest stem cells from human epidermis of aged donors maintain their multipotency in vitro and in vivo. Sci Rep 9 9750 PMID: 31278326

Sewanan et al (2019) Extracellular Matrix From Hypertrophic Myocardium Provokes Impaired Twitch Dynamics in Healthy Cardiomyocytes. JACC Basic Transl Sci 4 495 PMID: 31468004


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Reviews for IWP 4

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would use again.
By Anonymous on 05/10/2018
Species: Human

colleague used for an experiment


Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Pathways for IWP 4

Wnt

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.