IWP 4

Pricing Availability Delivery Time Qty
Cat.No. 5214 - IWP 4 | C23H20N4O3S3 | CAS No. 686772-17-8
Description: Potent inhibitor of Wnt/β-catenin signaling
Chemical Name: N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-3-(2-methoxyphenyl)-4-oxothieno[3,2-d]pyrimidin-2-yl)thio]-acetamide
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature
Pathways

Biological Activity

Potent inhibitor of Wnt/β-catenin signaling (IC50 = 25 nM). Has minimal effect on Notch and Hedgehog signaling pathways. Induces differentiation of cardiomyocytes from human ESCs and iPSCs.

Technical Data

M. Wt 496.62
Formula C23H20N4O3S3
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 686772-17-8
PubChem ID 2155264
InChI Key RHUJMHOIQBDFQR-UHFFFAOYSA-N
Smiles O=C1C3=C(CCS3)N=C(SCC(NC4=NC(C=CC(C)=C5)=C5S4)=O)N1C2=C(OC)C=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 4.97 10mM with sonication

Preparing Stock Solutions

The following data is based on the product molecular weight 496.62. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.01 mL 10.07 mL 20.14 mL
5 mM 0.4 mL 2.01 mL 4.03 mL
10 mM 0.2 mL 1.01 mL 2.01 mL
50 mM 0.04 mL 0.2 mL 0.4 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Lian et al (2012) Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc.Natl.Acad.Sci.U.S.A. 109 E1848-57 PMID: 22645348

Chen et al (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat.Chem.Biol. 5 100 PMID: 19125156

Narytnyk et al (2014) Differentiation of human epidermal neural crest stem cells (hEPI-NCSC) into virtually homogenous populations of dopaminergic neurons. Stem Cell Rev. 10 316 PMID: 24399192


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Citations for IWP 4

Citations are publications that use Tocris products.

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Reviews

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Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Pathways for IWP 4

Wnt

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.

Protocols

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