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Description: Potent inhibitor of Wnt/β-catenin signaling
Chemical Name: N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-3-(2-methoxyphenyl)-4-oxothieno[3,2-d]pyrimidin-2-yl)thio]-acetamide
Purity: ≥98% (HPLC)
Citations (15)
Pathways (1)

Biological Activity for IWP 4

IWP 4 is a potent inhibitor of Wnt/β-catenin signaling (IC50 = 25 nM). Has minimal effect on Notch and Hedgehog signaling pathways. Induces differentiation of cardiomyocytes from human ESCs and iPSCs.

Technical Data for IWP 4

M. Wt 496.62
Formula C23H20N4O3S3
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 686772-17-8
PubChem ID 2155264
Smiles O=C1C3=C(CCS3)N=C(SCC(NC4=NC(C=CC(C)=C5)=C5S4)=O)N1C2=C(OC)C=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for IWP 4

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 0.5 1

Preparing Stock Solutions for IWP 4

The following data is based on the product molecular weight 496.62. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.01 mM 201.36 mL 1006.81 mL 2013.61 mL
0.05 mM 40.27 mL 201.36 mL 402.72 mL
0.1 mM 20.14 mL 100.68 mL 201.36 mL
0.5 mM 4.03 mL 20.14 mL 40.27 mL

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Product Datasheets for IWP 4

Certificate of Analysis / Product Datasheet
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References for IWP 4

References are publications that support the biological activity of the product.

Lian et al (2012) Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc.Natl.Acad.Sci.U.S.A. 109 E1848-57 PMID: 22645348

Chen et al (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat.Chem.Biol. 5 100 PMID: 19125156

Narytnyk et al (2014) Differentiation of human epidermal neural crest stem cells (hEPI-NCSC) into virtually homogenous populations of DArgic neurons. Stem Cell Rev. 10 316 PMID: 24399192

Hoang et al (2018) Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells. Nat Protoc. 13 723 PMID: 29543795

Noor et al (2019) 3D Printing of Personalized Thick and Perfusable Cardiac Patches and Hearts. Adv Sci (Weinh) 6 1900344 PMID: 31179230

If you know of a relevant reference for IWP 4, please let us know.

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15 Citations for IWP 4

Citations are publications that use Tocris products. Selected citations for IWP 4 include:

Sewanan et al (2019) Extracellular Matrix From Hypertrophic Myocardium Provokes Impaired Twitch Dynamics in Healthy Cardiomyocytes. JACC Basic Transl Sci 4 495 PMID: 31468004

Boroujeni et al (2019) Neural crest stem cells from human epidermis of aged donors maintain their multipotency in vitro and in vivo. Sci Rep 9 9750 PMID: 31278326

Joshy et al (2020) Poison Exon Splicing Regulates a Coordinated Network of SR Protein Expression during Differentiation and Tumorigenesis. Mol Cell 80 648-665.e9 PMID: 33176162

Jonathan G et al (2018) Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions. Dev Cell 44 87-96.e5 PMID: 29316444

Wei et al (2018) iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications. Cell 173 851-863.e16 PMID: 29576452

Shiyong et al (2023) Lipid droplet-associated lncRNA LIPTER preserves cardiac lipid metabolism. Nat Cell Biol PMID: 37264180

Parisa et al (2021) Using hiPSC-CMs to Examine Mechanisms of Catecholaminergic Polymorphic Ventricular Tachycardia. Curr Protoc 1 e320 PMID: 34958715

Esra et al (2021) Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Methods Mol Biol 2520 161-170 PMID: 34845657

J Travis et al (2021) Actinin BioID reveals sarcomere crosstalk with oxidative metabolism through interactions with IGF2BP2. Cell Rep 36 109512 PMID: 34380038

Andrew et al (2021) SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway. J Virol 95 e0086221 PMID: 34260266

Paul et al (2021) Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment. Cell Rep 35 109088 PMID: 33951429

Wai Hoe et al (2022) Co-differentiation and Co-maturation of Human Cardio-pulmonary Progenitors and Micro-Tissues from Human Induced Pluripotent Stem Cells. Bio Protoc 12 e4488 PMID: 36199699

Christine E et al (2022) Transcription factor protein interactomes reveal genetic determinants in heart disease. Cell 185 794-814.e30 PMID: 35182466

Kamil et al (2022) Engineering a living cardiac pump on a chip using high-precision fabrication. Sci Adv 8 eabm3791 PMID: 35452278

Ming et al (2022) Recapitulating human cardio-pulmonary co-development using simultaneous multilineage differentiation of pluripotent stem cells. Elife 11 PMID: 35018887

Do you know of a great paper that uses IWP 4 from Tocris? Please let us know.

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Pathways for IWP 4

Wnt Signaling Pathway

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.