Indirubin-3'-oxime

Pricing Availability   Qty
Description: GSK-3β inhibitor; also inhibits cdks and other protein kinases
Alternative Names: Indirubin-3'-monoxime
Chemical Name: 3-[1,3-Dihydro-3-(hydroxyimino)-2H-indol-2-ylidene]-1,3-dihydro-2H-indol-2-one
Purity: ≥99% (HPLC)
Datasheet
Citations
Reviews
Literature (7)
Pathways (1)

Biological Activity for Indirubin-3'-oxime

Indirubin-3'-oxime is a inhibitor of GSK-3β (IC50 = 190 nM) and cyclin dependent kinases (cdks; IC50 values are 0.05, 0.18 and 0.25-0.5 βM for cdk9, cdk1 and cdk2, respectively). Also reported to inhibit AMPK, LCK and SGK. Inhibits cdk5- and GSK-3β-mediated tau phosphorylation. Induces cell cycle arrest and inhibits cell proliferation.

Technical Data for Indirubin-3'-oxime

M. Wt 277.28
Formula C16H11N3O2
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 160807-49-8
PubChem ID 5326739
InChI Key LDEWQRSYYHTQRA-UHFFFAOYSA-N
Smiles O\N=C1C2=CC=CC=C2NC/1=C1\C(=O)NC2=C1C=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Indirubin-3'-oxime

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 27.73 100
ethanol 27.73 100

Preparing Stock Solutions for Indirubin-3'-oxime

The following data is based on the product molecular weight 277.28. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.61 mL 18.03 mL 36.06 mL
5 mM 0.72 mL 3.61 mL 7.21 mL
10 mM 0.36 mL 1.8 mL 3.61 mL
50 mM 0.07 mL 0.36 mL 0.72 mL

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Product Datasheets for Indirubin-3'-oxime

References for Indirubin-3'-oxime

References are publications that support the biological activity of the product.

Bain et al (2003) The specificities of protein kinase inhibitors: an update. Biochem.J. 371 199 PMID: 12534346

Hoessel et al (1999) Indirubin, the active constituent of a Chinese antileukaemia medicine, inhibits cyclin-dependent kinases. Nat.Cell.Biol. 1 60 PMID: 10559866

Leclerc et al (2001) Indirubins inhibit glycogen synthase kinase-3β and CDK5/p25, two protein kinases involved in abnormal tau phosphorylation in Alzheimer's disease. J.Biol.Chem. 276 251 PMID: 11013232

Jorda et al (2018) How selective are pharmacological inhibitors of cell-cycle-regulating cyclin-dependent kinases? J.Med.Chem. 61 9105 PMID: 30234987


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Citations for Indirubin-3'-oxime

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Literature in this Area

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Pathways for Indirubin-3'-oxime

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