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Hoechst Janelia Fluor® 646 New
Biological Activity for Hoechst Janelia Fluor® 646
Hoechst Janelia Fluor® 646 is a fluorogenic red-emitting DNA probe; it preferentially stains and binds minor groove of AT-rich regions. The compound can be combined with fluorogenic green-emitting DNA probe Hoechst Janelia Fluor® 526 for multiplexing experiments. Hoechst Janelia Fluor® 646 can also be combined with Hoechst Janelia Fluor® 526 to perform dual-color STED using the same depletion laser (λdep = 775 nm). Sub-diffraction spatial resolution STED imaging can be achieved using Hoechst Janelia Fluor® 646 in E. coli cells (~100 nM - 1 μM).
Hoechst Janelia Fluor® 646 is a desirable alternative to large oligonucleotide-conjugated antibodies for PAINT experiments, particularly for bacterial studies. Hoechst Janelia Fluor® 646 exhibits a quantum yield (QY) of 0.54; extinction coefficicent 152, 000; excitation maximum = 655 nm; emission maximum = 670 nm.
Fluorogenic: fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor® 646 is suitable for multicolor microscopy experiments and for use in live cell imaging.
To measure the absorbance spectrum of Hoechst Janelia Fluo® 646 we recommend the following solvent: TFE plus 0.1% TFA.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Hoechst Janelia Fluor® 646
Technical Data for Hoechst Janelia Fluor® 646
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Hoechst Janelia Fluor® 646
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Hoechst Janelia Fluor® 646
The following data is based on the product molecular weight 1119.39. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||8.93 mL||44.67 mL||89.33 mL|
|0.5 mM||1.79 mL||8.93 mL||17.87 mL|
|1 mM||0.89 mL||4.47 mL||8.93 mL|
|5 mM||0.18 mL||0.89 mL||1.79 mL|
Product Datasheets for Hoechst Janelia Fluor® 646
References for Hoechst Janelia Fluor® 646
References are publications that support the biological activity of the product.
Legant et al (2016) High density three-dimensional localization microscopy across large volumes. Nat.Methods 13 359 PMID: 26950745
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757
Saphn et al Bleaching-independent, whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores. BioRxiv - Paper not yet peer reviewed
Spahn et al (2019) Whole-Cell, 3D, and multicolor STED imaging with exchangeable fluorophores. Nano Lett. 19 500 PMID: 30525682
Spahn et al (2018) A toolbox for multiplexed super-resolution imaging of the E. coli nucleoid and membrane using novel PAINT labels. Sci. Rep. 8 14768 PMID: 30282984
If you know of a relevant reference for Hoechst Janelia Fluor® 646, please let us know.
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Fluorescent Probes and Dyes Research Product Guide
This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes
- Fluorescent Dyes
- Dyes for Flow Cytometry
- Fluorescent Probes
- Anti-fade Reagents
- Bioluminescent Substrates
- Fluorogenic Dyes for Light-Up Aptamers
- Fluorescent Probes for Imaging Bacteria
- TSA Reagents for Enhancing IHC, ICC & FISH Signals