Hoechst Janelia Fluor® 526

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Cat.No. 7313 - Hoechst Janelia Fluor 526 | C62H58F6N10O8.4CF3CO2H
Description: Fluorogenic, green-emitting DNA probe
Alternative Names: JF526-HST
Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-((2-(2-(2-(4-(4-(6-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate tetratrifluoroacetate
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity for Hoechst Janelia Fluor® 526

Hoechst Janelia Fluor® 526 is a fluorogenic, green-emitting DNA probe. Preferentially stains and binds minor groove of AT-rich regions. When bound to A-T DNA, Hoechst Janelia Fluor® 526 exhibits a quantum yield (QY) of 0.126; when bound to G-C DNA Hoechst Janelia Fluor® 526 exhibits a quantum yield = 0.04.

Fluorogenic: fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor® 526 is suitable for multicolor microscopy experiments and for use in live cell imaging.

Excitation maximum = 531 nm; emission maximum = 549 nm.

To measure the absorbance spectrum of Hoechst Janelia Fluor® 526 we recommend the following solvent: TFE plus 0.1% TFA.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Hoechst Janelia Fluor® 526

Hoechst Janelia Fluor<sup>®</sup> 526 Dye Spectra
Emission Color Green
λabs 531 nm
λem 549 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.126
Application Confocal, Super-resolution microscopy

Technical Data for Hoechst Janelia Fluor® 526

M. Wt 1641.27
Formula C62H58F6N10O8.4CF3CO2H
Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key AFTKOWWBAAQRNW-UHFFFAOYSA-N
Smiles CN1CCN(CC1)C2=CC=C3N=C(NC3=C2)C4=CC=C5N=C(NC5=C4)C6=CC=C(OCCCC(NCCOCCOCCNC(C7=CC(C8=C9C=C(F)/C(C=C9OC%10=C8C=C(F)C(N%11CC(C%11)(F)F)=C%10)=[N+]%12CC(C\%12)(F)F)=C(C=C7)C([O-])=O)=O)=O)C=C6.O=C(C(F)(F)F)O.O=C(C(F)(F)F)O.O=C(C(F)(F)F)O.O=C(C(F)(F)F)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Hoechst Janelia Fluor® 526

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 12.99 10

Preparing Stock Solutions for Hoechst Janelia Fluor® 526

The following data is based on the product molecular weight 1641.27. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 6.09 mL 30.46 mL 60.93 mL
0.5 mM 1.22 mL 6.09 mL 12.19 mL
1 mM 0.61 mL 3.05 mL 6.09 mL
5 mM 0.12 mL 0.61 mL 1.22 mL

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Product Datasheets for Hoechst Janelia Fluor® 526

References for Hoechst Janelia Fluor® 526

References are publications that support the biological activity of the product.

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787


If you know of a relevant reference for Hoechst Janelia Fluor® 526, please let us know.

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Citations for Hoechst Janelia Fluor® 526

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