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Hoechst Janelia Fluor® 526 New
Biological Activity for Hoechst Janelia Fluor® 526
Hoechst Janelia Fluor® 526 is a fluorogenic, green-emitting DNA probe. Preferentially stains and binds minor groove of AT-rich regions. When bound to A-T DNA, Hoechst Janelia Fluor® 526 exhibits a quantum yield (QY) of 0.126; when bound to G-C DNA Hoechst Janelia Fluor® 526 exhibits a quantum yield = 0.04.
Fluorogenic: fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor® 526 is suitable for multicolor microscopy experiments and for use in live cell imaging.
Excitation maximum = 531 nm; emission maximum = 549 nm.
To measure the absorbance spectrum of Hoechst Janelia Fluor® 526 we recommend the following solvent: TFE plus 0.1% TFA.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Hoechst Janelia Fluor® 526
Technical Data for Hoechst Janelia Fluor® 526
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Hoechst Janelia Fluor® 526
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Hoechst Janelia Fluor® 526
The following data is based on the product molecular weight 1641.27. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||6.09 mL||30.46 mL||60.93 mL|
|0.5 mM||1.22 mL||6.09 mL||12.19 mL|
|1 mM||0.61 mL||3.05 mL||6.09 mL|
|5 mM||0.12 mL||0.61 mL||1.22 mL|
Product Datasheets for Hoechst Janelia Fluor® 526
References for Hoechst Janelia Fluor® 526
References are publications that support the biological activity of the product.
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
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Fluorescent Probes and Dyes Research Product Guide
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