Hoechst Janelia Fluor® 526

Pricing Availability   Qty

Save up to 40% on RUO Reagents with BIOSPRING24 (see details)

Description: Fluorogenic, green-emitting DNA probe
Alternative Names: JF526-HST
Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-((2-(2-(2-(4-(4-(6-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate tetratrifluoroacetate
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for Hoechst Janelia Fluor® 526

Hoechst Janelia Fluor® 526 is a fluorogenic, green-emitting DNA probe. Preferentially stains and binds minor groove of AT-rich regions. When bound to A-T DNA, Hoechst Janelia Fluor® 526 exhibits a quantum yield (QY) of 0.126; when bound to G-C DNA Hoechst Janelia Fluor® 526 exhibits a quantum yield = 0.04.

Fluorogenic: fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor® 526 is suitable for multicolor microscopy experiments and for use in live cell imaging.

Excitation maximum = 531 nm; emission maximum = 549 nm.

To measure the absorbance spectrum of Hoechst Janelia Fluor® 526 we recommend the following solvent: TFE plus 0.1% TFA.

Scientific Data

Confocal Microscopy Application of Hoechst Janelia Fluor<sup>®</sup> 526 in U2OS cells. View Larger

Application of Hoechst Janelia Fluor® 526 in U2OS cells. Fixed U2OS cells were stained with Hoechst Janelia Fluor® 526 (2 µM) for 1 hour and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Hoechst Janelia Fluor® 526

Hoechst Janelia Fluor<sup>®</sup> 526 Dye Spectra
Emission Color Yellow
λabs 531 nm
λem 549 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.126
Application Confocal Microscopy, Super Resolution Microscopy (SRM)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for Hoechst Janelia Fluor® 526

M. Wt 1641.27
Formula C62H58F6N10O8.4CF3CO2H
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles CN1CCN(CC1)C2=CC=C3N=C(NC3=C2)C4=CC=C5N=C(NC5=C4)C6=CC=C(OCCCC(NCCOCCOCCNC(C7=CC(C8=C9C=C(F)/C(C=C9OC%10=C8C=C(F)C(N%11CC(C%11)(F)F)=C%10)=[N+]%12CC(C\%12)(F)F)=C(C=C7)C([O-])=O)=O)=O)C=C6.O=C(C(F)(F)F)O.O=C(C(F)(F)F)O.O=C(C(F)(F)F)O.O=C(C(F)(F)F)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Hoechst Janelia Fluor® 526

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 12.99 10

Preparing Stock Solutions for Hoechst Janelia Fluor® 526

The following data is based on the product molecular weight 1641.27. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 6.09 mL 30.46 mL 60.93 mL
0.5 mM 1.22 mL 6.09 mL 12.19 mL
1 mM 0.61 mL 3.05 mL 6.09 mL
5 mM 0.12 mL 0.61 mL 1.22 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Hoechst Janelia Fluor® 526

References for Hoechst Janelia Fluor® 526

References are publications that support the biological activity of the product.

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for Hoechst Janelia Fluor® 526, please let us know.

Keywords: Hoechst Janelia Fluor 526, Hoechst Janelia Fluor 526 supplier, Hoechst, Janelia, Fluor, 526, DNA, stains, stain, dyes, probes, fluorogenic, green-emitting, live, cell, imaging, JF526-HST, Fluorescent, Stains, Flow, Cytometry, Counterstains, 7313, Tocris Bioscience

Citations for Hoechst Janelia Fluor® 526

Citations are publications that use Tocris products.

Currently there are no citations for Hoechst Janelia Fluor® 526. Do you know of a great paper that uses Hoechst Janelia Fluor® 526 from Tocris? Please let us know.

Reviews for Hoechst Janelia Fluor® 526

There are currently no reviews for this product. Be the first to review Hoechst Janelia Fluor® 526 and earn rewards!

Have you used Hoechst Janelia Fluor® 526?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals