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Biological Activity for H2DCFDA
H2DCFDA is a fluorescent ROS indicator; becomes fluorescent when oxidized. Excitation wavelength ~490 nm, emission ~520 nm. Cell permeable.
Technical Data for H2DCFDA
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for H2DCFDA
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for H2DCFDA
The following data is based on the product molecular weight 487.29. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.05 mL||10.26 mL||20.52 mL|
|5 mM||0.41 mL||2.05 mL||4.1 mL|
|10 mM||0.21 mL||1.03 mL||2.05 mL|
|50 mM||0.04 mL||0.21 mL||0.41 mL|
References for H2DCFDA
References are publications that support the biological activity of the product.
Nicholson et al (2013) Sigma-2 receptors play a role in cellular metabolism: stimulation of glycolytic hallmarks by CM764 in human SK-N-SH neuroblastoma. J.Pharmacol.Exp.Ther. 356 232 PMID: 26574517
Santabárbara-Ruiz et al (2015) ROS-induced JNK and p38 signaling is required for unpaired cytokine activation during drosophila regeneration. PLoS Genet. 11 e1005595 PMID: 26496642
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Citations for H2DCFDA
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Reviews for H2DCFDA
Average Rating: 5 (Based on 2 Reviews.)
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Glioblatsoma cell line G7 was cultured in 3D sphere model and the levels of intracellular ROS were measured.
Try different dilutions to get the one best suited to you.We treated the cells for 30 minutes and that was enough to get robust results
Literature in this Area
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