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Description: Fluorescent ROS indicator; cell permeable
Chemical Name: 2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid
Purity: ≥95% (HPLC)
Citations (2)
Reviews (2)
Literature (1)

Biological Activity for H2DCFDA

H2DCFDA is a fluorescent ROS indicator; becomes fluorescent when oxidized. Excitation wavelength ~490 nm, emission ~520 nm. Cell permeable.

Technical Data for H2DCFDA

M. Wt 487.29
Formula C24H16Cl2O7
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 4091-99-0
PubChem ID 77718
Smiles ClC1=CC(C(C2=CC=CC=C2C(O)=O)C(C=C(Cl)C(OC(C)=O)=C3)=C3O4)=C4C=C1OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for H2DCFDA

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 48.73 100
ethanol 4.87 10

Preparing Stock Solutions for H2DCFDA

The following data is based on the product molecular weight 487.29. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.05 mL 10.26 mL 20.52 mL
5 mM 0.41 mL 2.05 mL 4.1 mL
10 mM 0.21 mL 1.03 mL 2.05 mL
50 mM 0.04 mL 0.21 mL 0.41 mL

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Product Datasheets for H2DCFDA

Certificate of Analysis / Product Datasheet
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References for H2DCFDA

References are publications that support the biological activity of the product.

Nicholson et al (2013) Sigma-2 receptors play a role in cellular metabolism: stimulation of glycolytic hallmarks by CM764 in human SK-N-SH neuroblastoma. J.Pharmacol.Exp.Ther. 356 232 PMID: 26574517

Santabárbara-Ruiz et al (2015) ROS-induced JNK and p38 signaling is required for unpaired cytokine activation during drosophila regeneration. PLoS Genet. 11 e1005595 PMID: 26496642

If you know of a relevant reference for H2DCFDA, please let us know.

Keywords: H2DCFDA, H2DCFDA supplier, Cell, permeable, fluorescent, probes, ROS, reactive, oxygen, species, indicators, dyes, Fluorescent, Indicators, and, Sensors, Donors, Flow, Cytometry, 5935, Tocris Bioscience

2 Citations for H2DCFDA

Citations are publications that use Tocris products. Selected citations for H2DCFDA include:

Walter F et al (2022) CRISPR-Cas9 screen identifies oxidative phosphorylation as essential for cancer cell survival at low extracellular pH. Cell Rep 38 110493 PMID: 35263578

Andris et al (2022) Oxidative stress from DGAT1 oncoprotein inhibition in melanoma suppresses tumor growth when ROS defenses are also breached. Cell Rep 39 110995 PMID: 35732120

Do you know of a great paper that uses H2DCFDA from Tocris? Please let us know.

Reviews for H2DCFDA

Average Rating: 5 (Based on 2 Reviews.)

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H2DCFDA detects intracellular ROS levels in 3D Glioblastoma cell lines.
By sara Azeem on 01/08/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: G7

Glioblatsoma cell line G7 was cultured in 3D sphere model and the levels of intracellular ROS were measured.

Try different dilutions to get the one best suited to you.We treated the cells for 30 minutes and that was enough to get robust results

review image

H2DCFDA detects intracellular ROS levels in 15d-PGJ2 treated cardiomyocytes.
By Chintan Koyani on 10/13/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: HL-1 cardiomyocytes

HL-1 cardiomyocytes were stimulated with 15d-PGJ2 up to 60 min to follow intracellular ROS levels using H2DCFDA.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

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