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Cat.No. 5935 - H2DCFDA | C24H16Cl2O7 | CAS No. 4091-99-0
Description: Fluorescent ROS indicator; cell permeable
Chemical Name: 2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid
Purity: ≥97% (HPLC)

Biological Activity

Fluorescent ROS indicator; becomes fluorescent when oxidized. Excitation wavelength ~490 nm, emission ~520 nm. Cell permeable.

Technical Data

M. Wt 487.29
Formula C24H16Cl2O7
Storage Store at -20°C
Purity ≥97% (HPLC)
CAS Number 4091-99-0
PubChem ID 77718
Smiles ClC1=CC(C(C2=CC=CC=C2C(O)=O)C(C=C(Cl)C(OC(C)=O)=C3)=C3O4)=C4C=C1OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 48.73 100
ethanol 4.87 10

Preparing Stock Solutions

The following data is based on the product molecular weight 487.29. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.05 mL 10.26 mL 20.52 mL
5 mM 0.41 mL 2.05 mL 4.1 mL
10 mM 0.21 mL 1.03 mL 2.05 mL
50 mM 0.04 mL 0.21 mL 0.41 mL

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Product Datasheets

Safety Datasheet


References are publications that support the products' biological activity.

Nicholson et al (2013) Sigma-2 receptors play a role in cellular metabolism: stimulation of glycolytic hallmarks by CM764 in human SK-N-SH neuroblastoma. J.Pharmacol.Exp.Ther. 356 232 PMID: 26574517

Santabárbara-Ruiz et al (2015) ROS-induced JNK and p38 signaling is required for unpaired cytokine activation during drosophila regeneration. PLoS Genet. 11 e1005595 PMID: 26496642

If you know of a relevant reference for H2DCFDA, please let us know.

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Citations for H2DCFDA

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Literature in this Area

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New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

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Pathways for H2DCFDA


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