H2DCFDA

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Cat.No. 5935 - H2DCFDA | C24H16Cl2O7 | CAS No. 4091-99-0
Description: Fluorescent ROS indicator; cell permeable
Chemical Name: 2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid
Purity: ≥97% (HPLC)
Datasheet
Citations
Reviews (1)
Literature (1)

Biological Activity

Fluorescent ROS indicator; becomes fluorescent when oxidized. Excitation wavelength ~490 nm, emission ~520 nm. Cell permeable.

Technical Data

M. Wt 487.29
Formula C24H16Cl2O7
Storage Store at -20°C
Purity ≥97% (HPLC)
CAS Number 4091-99-0
PubChem ID 77718
InChI Key PXEZTIWVRVSYOK-UHFFFAOYSA-N
Smiles ClC1=CC(C(C2=CC=CC=C2C(O)=O)C(C=C(Cl)C(OC(C)=O)=C3)=C3O4)=C4C=C1OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 48.73 100
ethanol 4.87 10

Preparing Stock Solutions

The following data is based on the product molecular weight 487.29. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.05 mL 10.26 mL 20.52 mL
5 mM 0.41 mL 2.05 mL 4.1 mL
10 mM 0.21 mL 1.03 mL 2.05 mL
50 mM 0.04 mL 0.21 mL 0.41 mL

Molarity Calculator

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References

References are publications that support the biological activity of the product.

Nicholson et al (2013) Sigma-2 receptors play a role in cellular metabolism: stimulation of glycolytic hallmarks by CM764 in human SK-N-SH neuroblastoma. J.Pharmacol.Exp.Ther. 356 232 PMID: 26574517

Santabárbara-Ruiz et al (2015) ROS-induced JNK and p38 signaling is required for unpaired cytokine activation during drosophila regeneration. PLoS Genet. 11 e1005595 PMID: 26496642


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Keywords: H2DCFDA, H2DCFDA supplier, Cell, permeable, fluorescent, probes, ROS, reactive, oxygen, species, indicators, dyes, Fluorescent, Indicators, and, Sensors, Donors, 5935, Tocris Bioscience

Citations for H2DCFDA

Citations are publications that use Tocris products.

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Reviews for H2DCFDA

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H2DCFDA detects intracellular ROS levels in 15d-PGJ2 treated cardiomyocytes.
By Chintan Koyani on 10/13/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: HL-1 cardiomyocytes

HL-1 cardiomyocytes were stimulated with 15d-PGJ2 up to 60 min to follow intracellular ROS levels using H2DCFDA.

review image

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