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Protein kinase inhibitor. IC50 values for inhibition of PKC, PKG, PKA and myosin light chain kinase are 6.0, 5.8, 3.0 and 97.0 μM respectively.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 364.29. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.75 mL||13.73 mL||27.45 mL|
|5 mM||0.55 mL||2.75 mL||5.49 mL|
|10 mM||0.27 mL||1.37 mL||2.75 mL|
|50 mM||0.05 mL||0.27 mL||0.55 mL|
References are publications that support the biological activity of the product.
Hidaka et al (1984) Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry 23 5036 PMID: 6238627
Quick et al (1992) The structure and biological activities of the widely used protein kinase inhibitor, H7, differ depending on the commercial source. Biochem.Biophys.Res.Commun. 187 657 PMID: 1530632
Ratz et al (1990) Effect of the kinase inhibitor, H-7, on stress, crossbridge phosphorylation, muscle shortening and inositol phosphate production in rabbit arteries. J.Pharmacol.Exp.Ther. 252 253 PMID: 2299593
If you know of a relevant reference for H-7 dihydrochloride, please let us know.
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Keywords: H-7 dihydrochloride, H-7 dihydrochloride supplier, Protein, kinase, Broad, Spectrum, inhibitors, inhibits, Kinase, Inhibitors, 0542, Tocris Bioscience
4 Citations for H-7 dihydrochloride
Citations are publications that use Tocris products. Selected citations for H-7 dihydrochloride include:
Zhao et al (2018) TRPV4 Blockade Preserves the Blood-Brain Barrier by Inhibiting Stress Fiber Formation in a Rat Model of Intracerebral Hemorrhage. Front Mol Neurosci 11 97 PMID: 29636662
Belmeguenai and Hansel (2005) A role for protein phosphatases 1, 2A, and 2B in cerebellar long-term potentiation. Mol Pharmacol 25 10768 PMID: 16291950
Ludgate et al (2012) Cyclin-dependent kinase 2 phosphorylates s/t-p sites in the hepadnavirus core protein C-terminal domain and is incorporated into viral capsids. J Virol 86 12237 PMID: 22951823
Barcomb et al (2013) Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B. Front Mol Neurosci 84 834 PMID: 24056996
Do you know of a great paper that uses H-7 dihydrochloride from Tocris? Please let us know.
Reviews for H-7 dihydrochloride
Average Rating: 5 (Based on 1 Review.)
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H7 was studied for its inhibitory role in cAMP dependent protein kinase with ICso values of 18 and 16 gM respectively.
Cultures were incubated for 16 hours at 37 ~ in an atmosphere of 5% CO2 and 95% humidified air. 1~tCi of [3H]-thymidine was added to each well and cultures incubated for a further 5 hours. Cells were then harvested onto glass fibre filters, washed and incorporated radioactivity determined by liq- uid scintillation spectrometry.
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