Phorbol 12-myristate 13-acetate
Extensively used phorbol ester activator of protein kinase C; tumor promoter. Binds to PKC with a Ki value of 2.6 nM as measured by displacement of [3H]phorbol 12,13-dibutyrate binding in rat cortex synaptosomal membranes.
|Storage||Desiccate at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 616.83. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.62 mL||8.11 mL||16.21 mL|
|5 mM||0.32 mL||1.62 mL||3.24 mL|
|10 mM||0.16 mL||0.81 mL||1.62 mL|
|50 mM||0.03 mL||0.16 mL||0.32 mL|
References are publications that support the biological activity of the product.
Castagna et al (1982) Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. J.Biol.Chem. 257 7847 PMID: 7085651
Hockberger et al (1989) A diacylglycerol analogue reduces neuronal calcium currents independently of protein kinase C activation. Nature 338 340 PMID: 2922062
Murphy et al (1999) Structural determinants of phorbol ester binding in synaptosomes: pharmacokinetics and pharmacodynamics. Eur.J.Pharmacol. 381 77 PMID: 10528137
Merck Index 12 7487
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Keywords: Phorbol 12-myristate 13-acetate, Phorbol 12-myristate 13-acetate supplier, Protein, kinases, C, activators, PKC, Phorbol12-myristate13-acetate, PMA, Kinase, 1201, Tocris Bioscience
18 Citations for Phorbol 12-myristate 13-acetate
Citations are publications that use Tocris products. Selected citations for Phorbol 12-myristate 13-acetate include:
Kaszás et al (2012) Small molecule positive allosteric modulation of TRPV1 activation by vanilloids and acidic pH. J Neurosci 340 152 PMID: 22005042
Gao et al (2012) Transient receptor potential vanilloid type 1-dependent regulation of liver-related neurons in the paraventricular nucleus of the hypothalamus diminished in the type 1 diabetic mouse. J Virol 61 1381 PMID: 22492526
Gabriel et al (2012) The acid-sensitive, anesthetic-activated potassium leak channel, KCNK3, is regulated by 14-3-3β-dependent, protein kinase C (PKC)-mediated endocytic trafficking. J Biol Chem 287 32354 PMID: 22846993
Jones et al (2004) Regulation of SHP-1 tyrosine phosphatase in human platelets by serine phosphorylation at its C terminus. Biochem Biophys Res Commun 279 40475 PMID: 15269224
Quizon (2016) Molecular mechanism: the human dopamine transporter histidine 547 regulates basal and HIV-1 Tatprotein-inhibited dopamine transport. Scientific Reports 6 39048 PMID: 27966610
Kapfhammer and Gugger (2012) The analysis of purkinje cell dendritic morphology in organotypic slice cultures. J Biol Chem NULL PMID: 22473312
Brust et al (2015) New functional activity of aripiprazole revealed: Robust antagonism of D2 dopamine receptor-stimulated Gβγ signaling. J Vis Exp 93 85 PMID: 25449598
Nishida et al (2009) Inhibition of ATR protein kinase activity by schisandrin B in DNA damage response. Nucleic Acids Res 37 5678 PMID: 19625493
Davis (2015) The ATP-Binding Cassette Transporter-2 (ABCA2) Overexpression Modulates Sphingosine Levels and Transcription of the Amyloid Precursor Protein (APP) Gene. Biochem Pharmacol 12 847 PMID: 26510981
Specht et al (2011) Regulation of glycine receptor diffusion properties and gephyrin interactions by protein kinase C. EMBO J 30 3842 PMID: 21829170
Navaroli et al (2011) The plasma membrane-associated GTPase Rin interacts with the dopamine transporter and is required for protein kinase C-regulated dopamine transporter trafficking. J Neurosci 31 13758 PMID: 21957239
Brown et al (2014) Mitogen-activated protein kinase-interacting kinase regulates mTOR/AKT signaling and controls the serine/arginine-rich protein kinase-responsive type 1 internal ribosome entry site-mediated translation and viral oncolysis. J Pharmacol Exp Ther 88 13149 PMID: 25187540
Hagen et al (2015) Comparative Multi-Donor Study of IFNγ Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays. Cells 4 84 PMID: 25679284
Bemben et al (2015) Autism-associated mutation inhibits protein kinase C-mediated neuroligin-4X enhancement of excitatory synapses. Proc Natl Acad Sci U S A 112 2551 PMID: 25675530
Walsh et al (2014) The role of Nox1 and Nox2 in GPVI-dependent platelet activation and thrombus formation. Redox Biol 2 178 PMID: 24494191
Yu et al (2014) Transactivation of epidermal growth factor receptor through platelet-activating factor/receptor in ovarian cancer cells. J Exp Clin Cancer Res 33 85 PMID: 25261977
Zsombok et al (2011) Functional plasticity of central TRPV1 receptors in brainstem dorsal vagal complex circuits of streptozotocin-treated hyperglycemic mice. Curr Alzheimer Res 31 14024 PMID: 21957263
Bohnekamp et al (2010) Involvement of the chemokine-like receptor GPR33 in innate immunity. Br J Pharmacol 396 272 PMID: 20399748
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50 ng/ml PMA used to stimulate cells isolated from mesenteric lymphnodes, followed by staining for IL-17A
Literature in this Area
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